scholarly journals Delimiting the Function of the C-Terminal Extension of the Escherichia coli [NiFe]-Hydrogenase 2 Large Subunit Precursor

2019 ◽  
Vol 10 ◽  
Author(s):  
Constanze Pinske ◽  
Claudia Thomas ◽  
Kerstin Nutschan ◽  
R. Gary Sawers
Keyword(s):  
Escherichia Coli ◽  
Large Subunit ◽  
Hydrogenase 2

scholarly journals Interplay between the Specific Chaperone-Like Proteins HybG and HypC in Maturation of Hydrogenases 1, 2, and 3 from Escherichia coli

2001 ◽  
Vol 183 (9) ◽  
pp. 2817-2822 ◽  
Author(s):  
Melanie Blokesch ◽  
Axel Magalon ◽  
August Böck
Keyword(s):  
Escherichia Coli ◽  
Complex Formation ◽  
Gene Product ◽  
Genetic Background ◽  
Large Subunit ◽  
Cysteine Residue ◽  
Maturation Process ◽  
Hydrogenase 3 ◽  
Hydrogenase 2

ABSTRACT The hybG gene product from Escherichia colihas been identified as a chaperone-like protein acting in the maturation of hydrogenases 1 and 2. It was shown that HybG forms a complex with the precursor of the large subunit of hydrogenase 2. As with HypC, which is the chaperone-like protein involved in hydrogenase 3 maturation, the N-terminal cysteine residue is crucial for complex formation. Introduction of a deletion into hybG abolished the generation of active hydrogenase 2 but only quantitatively reduced hydrogenase 1 activity since HypC could replace HybG in this function. In contrast, HybG could not take over the role of HypC in a ΔhypC genetic background. Overproduction of HybG, especially of the variants with the replaced N-terminal cysteine residue, strongly interfered with hydrogenase 3 maturation, apparently by titrating some other component(s) of the maturation machinery. The results indicate that the three hydrogenase isoenzymes not only are interacting at the functional level but are also interconnected during the maturation process.

Effect of alteration of the C-terminal extension on the maturation and folding of the large subunit of the Escherichia coli hydrogenase-2

Biochimie ◽  
2003 ◽  
Vol 85 (6) ◽  
pp. 575-579 ◽  
Author(s):  
Ming Zhang ◽  
Nathalie Pradel ◽  
Marie-Andrée Mandrand-Berthelot ◽  
Zengliang Yu ◽  
Long-Fei Wu
Keyword(s):  
Escherichia Coli ◽  
Large Subunit ◽  
Hydrogenase 2

scholarly journals Escherichia coli exonuclease VII. Cloning and sequencing of the gene encoding the large subunit (xseA).

1986 ◽  
Vol 261 (32) ◽  
pp. 14929-14935
Author(s):  
J W Chase ◽  
B A Rabin ◽  
J B Murphy ◽  
K L Stone ◽  
K R Williams
Keyword(s):  
Escherichia Coli ◽  
Large Subunit ◽  
Cloning And Sequencing ◽  
Gene Encoding ◽  
Exonuclease Vii

Structure-function studies of the large subunit of ribonucleotide reductase from Escherichia coli

1988 ◽  
Vol 16 (2) ◽  
pp. 91-94 ◽  
Author(s):  
OLLE NILSSON ◽  
TOMAS LUNDQVIST ◽  
SOLVEIG HAHNE ◽  
BRITT-MARIE SJÖBERG
Keyword(s):  
Escherichia Coli ◽  
Structure Function ◽  
Ribonucleotide Reductase ◽  
Large Subunit

scholarly journals Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2.

1994 ◽  
Vol 176 (14) ◽  
pp. 4416-4423 ◽  
Author(s):  
N K Menon ◽  
C Y Chatelus ◽  
M Dervartanian ◽  
J C Wendt ◽  
K T Shanmugam ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Mutational Analysis ◽  
Hydrogenase 2

Cloning and Expression of Influenza Virus A (H1N1) Haemagglutinin large subunit in Escherichia coli

2010 ◽  
Vol 150 ◽  
pp. 563-563
Author(s):  
B. Farahmand ◽  
M. Khodabandeh ◽  
F. Mahboodi ◽  
F. Fotouhi ◽  
M. Saleh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Influenza Virus ◽  
Large Subunit ◽  
Cloning And Expression ◽  
Influenza Virus A
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