scholarly journals Single-Element Reflective Digital Holographic Microscopy

2021 ◽  
Vol 9 ◽  
Author(s):  
José Ángel Picazo-Bueno ◽  
Javier García ◽  
Vicente Micó
Keyword(s):  
Low Cost ◽  
Digital Holographic Microscopy ◽  
Single Element ◽  
Phase Imaging ◽  
Single Shot ◽  
One Dimensional ◽  
Common Path ◽  
Microscopy Technique ◽  
Digital Holograms ◽  
Holographic Microscopy

Digital holographic microscopy (DHM) is a well-known microscopy technique using an interferometric architecture for quantitative phase imaging (QPI) and it has been already implemented utilizing a large number of interferometers. Among them, single-element interferometers are of particular interest due to its simplicity, stability, and low cost. Here, we present an extremely simple common-path interferometric layout based on the use of a single one-dimensional diffraction grating for both illuminating the sample in reflection and generating the digital holograms. The technique, named single-element reflective digital holographic microscopy (SER-DHM), enables QPI and topography analysis of reflective/opaque objects using a single-shot operation principle. SER-DHM is experimentally validated involving different reflective samples.

scholarly journals 3D imaging using scanning diffractometry

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Morteza J. Siavashani ◽  
Iman Naghedi ◽  
Vahid Abbasian ◽  
Ehsan A. Akhlaghi ◽  
Mohammad A. Charsooghi ◽  
...  
Keyword(s):  
3D Imaging ◽  
High Sensitivity ◽  
Digital Holographic Microscopy ◽  
Phase Imaging ◽  
Phase Step ◽  
3D Images ◽  
One Dimensional ◽  
Illuminating Light ◽  
Holographic Microscopy ◽  
Biomedical Measurements

AbstractImaging of cells is a challenging problem as they do not appreciably change the intensity of the illuminating light. Interferometry-based methods to do this task suffer from high sensitivity to environmental vibrations. We introduce scanning diffractometry as a simple non-contact and vibration-immune methodology for quantitative phase imaging. Fresnel diffractometry by a phase step has led to several applications such as high-precision measurements of displacement. Additional scanning may lead to 3D imaging straightforwardly. We apply the technique to acquire 3D images of holographic grating, red blood cell, neuron, and sperm cell. Either visibility of the diffraction fringes or the positions of extrema may be used for phase change detection. The theoretical analysis through the Fresnel diffraction from one-dimensional phase step is presented and the experimental results are validated with digital holographic microscopy. The presented technique can be suggested to serve as a robust device for 3D phase imaging and biomedical measurements.

Common-path, single-shot phase-shifting digital holographic microscopy using a Ronchi ruling

2019 ◽  
Vol 114 (18) ◽  
pp. 183701 ◽  
Author(s):  
S. Hossein S. Yaghoubi ◽  
Samira Ebrahimi ◽  
Masoomeh Dashtdar ◽  
Ana Doblas ◽  
Bahram Javidi
Keyword(s):  
Phase Shifting ◽  
Digital Holographic Microscopy ◽  
Single Shot ◽  
Common Path ◽  
Holographic Microscopy

scholarly journals Accurate single-shot quantitative phase imaging of biological specimens with telecentric digital holographic microscopy

2014 ◽  
Vol 19 (4) ◽  
pp. 046022 ◽  
Author(s):  
Ana Doblas ◽  
Emilio Sánchez-Ortiga ◽  
Manuel Martínez-Corral ◽  
Genaro Saavedra ◽  
Jorge Garcia-Sucerquia
Keyword(s):  
Digital Holographic Microscopy ◽  
Phase Imaging ◽  
Single Shot ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase ◽  
Holographic Microscopy
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