scholarly journals Single-Shot Through-Focus Image Acquisition and Phase Retrieval From Chromatic Aberration and Multi-Angle Illumination

2021 ◽  
Vol 9 ◽  
Author(s):  
Guocheng Zhou ◽  
Shaohui Zhang ◽  
Yayu Zhai ◽  
Yao Hu ◽  
Qun Hao

Phase recovery from a stack of through-focus intensity images is an effective non-interference quantitative phase imaging strategy. Nevertheless, the implementations of these methods are expensive and time-consuming because the distance between each through-focus plane has to be guaranteed by precision mechanical moving devices, and the multiple images must be acquired sequentially. In this article, we propose a single-shot through-focus intensity image stack acquisition strategy without any precision movement. Isolated LED units are used to illuminate the sample in different colors from different angles. Due to the chromatic aberration characteristics of the objective, the color-channel defocus images on the theoretical imaging plane are mutually laterally shifted. By calculating the shift amount of each sub-image area in each color channel, the distances between each through-focus image can be obtained, which is a critical parameter in transport of intensity equation (TIE) and alternating projection (AP). Lastly, AP is used to recover the phase distribution and realize the 3D localization of different defocus distances of the sample under test as an example. Both simulation and experiments are conducted to verify the feasibility of the proposed method.

2020 ◽  
Vol 28 (7) ◽  
pp. 9976
Author(s):  
Guocheng Zhou ◽  
Shaohui Zhang ◽  
Yao Hu ◽  
Qun Hao

2017 ◽  
Author(s):  
Yi Yang ◽  
Xuanzhe Zhang ◽  
Shaojun Du ◽  
Qiong Zhou ◽  
Quan Sun

2021 ◽  
Author(s):  
Péter Kocsis ◽  
Igor Shevkunov ◽  
Vladimir Katkovnik ◽  
Heikki Rekola ◽  
Karen Egiazarian

2021 ◽  
Vol 9 ◽  
Author(s):  
José Ángel Picazo-Bueno ◽  
Javier García ◽  
Vicente Micó

Digital holographic microscopy (DHM) is a well-known microscopy technique using an interferometric architecture for quantitative phase imaging (QPI) and it has been already implemented utilizing a large number of interferometers. Among them, single-element interferometers are of particular interest due to its simplicity, stability, and low cost. Here, we present an extremely simple common-path interferometric layout based on the use of a single one-dimensional diffraction grating for both illuminating the sample in reflection and generating the digital holograms. The technique, named single-element reflective digital holographic microscopy (SER-DHM), enables QPI and topography analysis of reflective/opaque objects using a single-shot operation principle. SER-DHM is experimentally validated involving different reflective samples.


Optica ◽  
2018 ◽  
Vol 5 (8) ◽  
pp. 976 ◽  
Author(s):  
Byounghyo Lee ◽  
Jong-young Hong ◽  
Dongheon Yoo ◽  
Jaebum Cho ◽  
Youngmo Jeong ◽  
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Keyword(s):  

2021 ◽  
Author(s):  
Mengqi Shen ◽  
Qi Zou ◽  
Xiaoping JIANG ◽  
Fu Feng ◽  
Michael Somekh

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