Reconstruction of Isolated Moving Objects by Motion-Induced Phase Shift Based on PSP

The reconstruction of moving objects based on phase shifting profilometry has attracted intensive interests. Most of the methods introduce the phase shift by projecting multiple fringe patterns, which is undesirable in moving object reconstruction as the errors caused by the motion will be intensified when the number of the fringe pattern is increased. This paper proposes the reconstruction of the isolated moving object by projecting two fringe patterns with different frequencies. The phase shift required by the phase shifting profilometry is generated by the object motion, and the model describing the motion-induced phase shift is presented. Then, the phase information in different frequencies is retrieved by analyzing the influence introduced by movement. Finally, the mismatch on the phase information between the two frequencies is compensated and the isolated moving object is reconstructed. Experiments are presented to verify the effectiveness of the proposed method.

Comparison of Two- and Three-Beam Interference Pattern Generation in Structured Illumination Microscopy

Structured illumination microscopy (SIM) provides wide-field optical sectioning in the focal plane by modulating the imaging information using fringe pattern illumination. For generating the fringe pattern illumination, a digital micro-mirror device (DMD) is commonly used due to its flexibility and fast refresh rate. However, the benefit of different pattern generation, for example, the two-beam interference mode and the three-beam interference mode, has not been clearly investigated. In this study, we systematically analyze the optical sectioning provided by the two-beam inference mode and the three-beam interference mode of DMD. The theoretical analysis and imaging results show that the two-beam interference mode is suitable for fast imaging of the superficial dynamic target due to reduced number of phase shifts needed to form the image, and the three-beam interference mode is ideal for imaging three-dimensional volume due to its superior optical sectioning by the improved modulation of the illumination patterns. These results, we believe, will provide better guidance for the use of DMD for SIM imaging and also for the choice of beam patterns in SIM application in the future.