Double-Clad Optical Fiber-Based Multi-Contrast Noncontact Photoacoustic and Fluorescence Imaging System

A noncontact photoacoustic and fluorescence dual-modality imaging system is proposed, which integrates a fiber-based fluorescence imaging system with noncontact photoacoustic imaging using a specially fabricated double-cladding fiber (DCF) coupler and a DCF lens. The performance of the DCF coupler and lens was evaluated, and the feasibility of this new imaging system was demonstrated using simple tubing phantoms with black ink and fluorophore. Our imaging results demonstrated that the multimodal imaging technique can simultaneously acquire photoacoustic and fluorescence images without coming into contact with the sample. Consequently, the developed method is the first noncontact scheme among multimodal imaging systems that is integrated with a photoacoustic imaging system, which can provide varied and complementary information about the sample.

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Democratising “Microscopi”: a 3D printed automated XYZT fluorescence imaging system for teaching, outreach and fieldwork

Wellcome Open Research ◽

10.12688/wellcomeopenres.16536.1 ◽

2021 ◽

Vol 6 ◽

pp. 63

Author(s):

Matthew Wincott ◽

Andrew Jefferson ◽

Ian M. Dobbie ◽

Martin J. Booth ◽

Ilan Davis ◽

...

Keyword(s):

Fluorescence Imaging ◽

Imaging System ◽

Imaging Systems ◽

Image Capture ◽

Optical Components ◽

Web Based ◽

Automated Imaging ◽

Fluorescence Imaging System ◽

3D Printed ◽

Imaging Performance

Commercial fluorescence microscope stands and fully automated XYZt fluorescence imaging systems are generally beyond the limited budgets available for teaching and outreach. We have addressed this problem by developing “Microscopi”, an accessible, affordable, DIY automated imaging system that is built from 3D printed and commodity off-the-shelf hardware, including electro-mechanical, computer and optical components. Our design features automated sample navigation and image capture with a simple web-based graphical user interface, accessible with a tablet or other mobile device. The light path can easily be switched between different imaging modalities. The open source Python-based control software allows the hardware to be driven as an integrated imaging system. Furthermore, the microscope is fully customisable, which also enhances its value as a learning tool. Here, we describe the basic design and demonstrate imaging performance for a range of easily sourced specimens.

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Clipping of an anterior spinal artery aneurysm using an endoscopic fluorescence imaging system for craniocervical junction epidural arteriovenous fistula: technical note

Journal of Neurosurgery Spine ◽

10.3171/2019.1.spine18983 ◽

2019 ◽

Vol 31 (2) ◽

pp. 279-284 ◽

Cited By ~ 2

Author(s):

Ahmed Mansour ◽

Toshiki Endo ◽

Tomoo Inoue ◽

Kenichi Sato ◽

Hidenori Endo ◽

...

Keyword(s):

Spinal Cord ◽

Arteriovenous Fistula ◽

Fluorescence Imaging ◽

Imaging System ◽

Artery Aneurysm ◽

Craniocervical Junction ◽

Anterior Spinal Artery ◽

Posterolateral Approach ◽

Fluorescence Imaging System ◽

Ventral Spinal Cord

The authors report the case of a 78-year-old man with a craniocervical junction epidural arteriovenous fistula who presented with subarachnoid hemorrhage from a ruptured anterior spinal artery (ASA) aneurysm. Because endovascular embolization was difficult, a posterolateral approach was chosen and a novel endoscopic fluorescence imaging system was utilized to clip the aneurysm. The fluorescence imaging system provided clear and magnified views of the ventral spinal cord simultaneously with the endoscope-integrated indocyanine green videoangiography, which helped safely obliterate the ASA aneurysm. With the aid of this novel imaging system, surgeons can appreciate and manipulate complex vascular pathologies of the ventral spinal cord through a posterolateral approach, even when the lesion is closely related to the ASA.

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