scholarly journals Antimicrobial Photodynamic Therapy in Combination with Nystatin in the Treatment of Experimental Oral Candidiasis Induced by Candida albicans Resistant to Fluconazole

2019 ◽  
Vol 12 (3) ◽  
pp. 140 ◽  
Author(s):  
Karem Janeth Rimachi Hidalgo ◽  
Juliana Cabrini Carmello ◽  
Cláudia Carolina Jordão ◽  
Paula Aboud Barbugli ◽  
Carlos Alberto de Sousa Costa ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Photodynamic Therapy ◽  
Combined Therapy ◽  
Oral Candidiasis ◽  
Confocal Scanning Laser Microscopy ◽  
Antimicrobial Photodynamic Therapy ◽  
Promising Alternative ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

Background: It has been demonstrated that azole-resistant strains of Candida albicans have a greater resistance to antimicrobial photodynamic therapy (aPDT) when compared to their more susceptible counterparts. For this reason, the present study evaluated the efficacy of aPDT, together with nystatin (NYS), in the treatment of oral candidiasis in vivo. Methods: Mice were infected with fluconazole-resistant C. albicans (ATCC 96901). To perform the combined therapy, aPDT, mediated by Photodithazine (PDZ) and LED light, was used together with NYS. The efficacy of the treatments was evaluated by microbiological, macroscopic, histopathological and Confocal Scanning Laser Microscopy analyses of the lesions. The expression of p21 and p53, proteins associated with cell death, from the tongues of mice, was also performed. Results: The combined therapy reduced the fungal viability by around 2.6 log10 and decreased the oral lesions and the inflammatory reaction. Additionally, it stimulated the production of p53 and p21. Conclusions: The combined therapy is a promising alternative treatment for oral candidiasis induced by C. albicans resistant to fluconazole.

scholarly journals In Vivo Confocal Scanning Laser Microscopy of Human Skin: Melanin Provides Strong Contrast

1995 ◽  
Vol 104 (6) ◽  
pp. 946-952 ◽  
Author(s):  
Milind Rajadhyaksha ◽  
Melanie Grossman ◽  
Dina Esterowitz ◽  
Robert H. Webb ◽  
R Rox Anderson
Keyword(s):  
Human Skin ◽  
Scanning Laser ◽  
Confocal Scanning Laser Microscopy ◽  
Laser Microscopy ◽  
Scanning Laser Microscopy ◽  
Confocal Scanning ◽  
Confocal Scanning Laser ◽  
Strong Contrast

scholarly journals In Vitro Activity of Caspofungin against Candida albicans Biofilms

2002 ◽  
Vol 46 (11) ◽  
pp. 3591-3596 ◽  
Author(s):  
Stefano P. Bachmann ◽  
Kacy VandeWalle ◽  
Gordon Ramage ◽  
Thomas F. Patterson ◽  
Brian L. Wickes ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Inhibitory Effect ◽  
Vitro Activity ◽  
Confocal Scanning Laser Microscopy ◽  
In Vitro Activity ◽  
Confocal Scanning ◽  
Confocal Scanning Laser ◽  
Biofilm Development ◽  
Candida Albicans Biofilms

ABSTRACT Most manifestations of candidiasis are associated with biofilm formation on biological or inanimate surfaces. Candida albicans biofilms are recalcitrant to treatment with conventional antifungal therapies. Here we report on the activity of caspofungin, a new semisynthetic echinocandin, against C. albicans biofilms. Caspofungin displayed potent in vitro activity against sessile C. albicans cells within biofilms, with MICs at which 50% of the sessile cells were inhibited well within the drug's therapeutic range. Scanning electron microscopy and confocal scanning laser microscopy were used to visualize the effects of caspofungin on preformed C. albicans biofilms, and the results indicated that caspofungin affected the cellular morphology and the metabolic status of cells within the biofilms. The coating of biomaterials with caspofungin had an inhibitory effect on subsequent biofilm development by C. albicans. Together these findings indicate that caspofungin displays potent activity against C. albicans biofilms in vitro and merits further investigation for the treatment of biofilm-associated infections.

Corneal In Vivo Confocal Scanning Laser Microscopy in Patients with Atopic Keratoconjunctivitis

Ophthalmology ◽  
2008 ◽  
Vol 115 (11) ◽  
pp. 2004-2012 ◽  
Author(s):  
Yiqian Hu ◽  
Yukihiro Matsumoto ◽  
Enrique Sato Adan ◽  
Murat Dogru ◽  
Kazumi Fukagawa ◽  
...  
Keyword(s):  
Scanning Laser ◽  
Confocal Scanning Laser Microscopy ◽  
Laser Microscopy ◽  
Atopic Keratoconjunctivitis ◽  
Scanning Laser Microscopy ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

Conjunctival In Vivo Confocal Scanning Laser Microscopy in Patients with Sjögren Syndrome

2010 ◽  
Vol 51 (1) ◽  
pp. 144 ◽  
Author(s):  
Tais Hitomi Wakamatsu ◽  
Enrique Adan Sato ◽  
Yukihiro Matsumoto ◽  
Osama M. A. Ibrahim ◽  
Murat Dogru ◽  
...  
Keyword(s):  
Sjögren Syndrome ◽  
Scanning Laser ◽  
Confocal Scanning Laser Microscopy ◽  
Sjogren Syndrome ◽  
Laser Microscopy ◽  
Scanning Laser Microscopy ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

scholarly journals The origin of extracellular DNA in bacterial biofilm infections in vivo

2020 ◽  
Vol 78 (2) ◽  
Author(s):  
Maria Alhede ◽  
Morten Alhede ◽  
Klaus Qvortrup ◽  
Kasper Nørskov Kragh ◽  
Peter Østrup Jensen ◽  
...  
Keyword(s):  
Polymorphonuclear Leukocytes ◽  
Bacterial Biofilm ◽  
Extracellular Dna ◽  
Confocal Scanning Laser Microscopy ◽  
Transmission Electron ◽  
Scanning Laser Microscopy ◽  
Confocal Scanning ◽  
Confocal Scanning Laser ◽  
Pna Fish

ABSTRACT Extracellular DNA (eDNA) plays an important role in both the aggregation of bacteria and in the interaction of the resulting biofilms with polymorphonuclear leukocytes (PMNs) during an inflammatory response. Here, transmission electron and confocal scanning laser microscopy were used to examine the interaction between biofilms of Pseudomonas aeruginosa and PMNs in a murine implant model and in lung tissue from chronically infected cystic fibrosis patients. PNA FISH, DNA staining, labeling of PMN DNA with a thymidine analogue and immunohistochemistry were applied to localize bacteria, eDNA, PMN-derived eDNA, PMN-derived histone H3 (H3), neutrophil elastase (NE) and citrullinated H3 (citH3). Host-derived eDNA was observed surrounding bacterial biofilms but not within the biofilms. H3 localized to the lining of biofilms while NE was found throughout biofilms. CitH3, a marker for neutrophil extracellular traps (NETs) was detected only sporadically indicating that most host-derived eDNA in vivo was not a result of NETosis. Together these observations show that, in these in vivo biofilm infections with P. aeruginosa, the majority of eDNA is found external to the biofilm and derives from the host.

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