scholarly journals Monitoring of Cardiorespiratory Parameters in Rats—Validation Based on Pharmacological Stimulation

2021 ◽  
Vol 14 (12) ◽  
pp. 1223
Author(s):  
Joanna Miklosz ◽  
Bartlomiej Kalaska ◽  
Stanislaw Zajaczkowski ◽  
Dariusz Pawlak ◽  
Andrzej Mogielnicki
Keyword(s):  
Blood Pressure ◽  
Body Temperature ◽  
Oxygen Saturation ◽  
Respiratory Rate ◽  
Blood Perfusion ◽  
Blood Oxygen ◽  
Blood Oxygen Saturation ◽  
Toxicity Studies ◽  
Respiratory Parameters ◽  
Safety And Toxicity

The methods used in preclinical studies should minimize the suffering and the number of animals but still provide precise and consistent results enabling the introduction of drug candidates into the phase of clinical trials. Thus, we aimed to develop a method allowing us to perform preliminary safety and toxicity studies of candidates for human medicines, while reducing the number of animals. We have devised a method based on a combination of two devices: Plugsys (Transonics System Inc., Ithaca, NY, USA) and PhysioSuite (Kent Scientific Corporation, Torrington, CT, USA), which allow simultaneous registration of nine circulatory and respiratory parameters, and body temperature. Vehicle and adrenaline, or nitroglycerin, as reference substances were administered into the right femoral vein of Wistar rats. Physiological conditions were registered over 60 min after drug administration by measuring systolic, diastolic and mean blood pressure, heart rate (HR), blood perfusion of paw vessels, blood oxygen saturation, respiratory rate, average and peak exhaled CO2, and body temperature. Blood pressure was measured by cannula placed in the left common carotid artery and connected to the pressure transducer (Plugsys). The other parameters were measured by the PhysioSuite. Adrenaline-induced immediate dose-related hypertension and nitroglycerin hypotension were correlated with the change in blood perfusion. They both increased HR. Adrenaline decreased blood oxygen saturation and slightly affected respiratory parameters, while nitroglycerin caused a progressive increase in respiratory rate and a decrease in the peak of exhaled CO2. Our method may become an inseparable part of the preliminary safety and toxicity studies of tested drugs, while being an important step towards improving animal welfare.

scholarly journals Effect of Local Cold Therapy on Arterial Blood Oxygen Saturation and Temperature Changes in Patients Undergoing Surgery

2021 ◽  
Vol 10 (3) ◽  
Author(s):  
Ali Miri ◽  
Mostafa Roshanzadeh ◽  
Reza Masoudi ◽  
Soleiman Kheiri ◽  
Shirmohammad Davoodvand
Keyword(s):  
Body Temperature ◽  
Oxygen Saturation ◽  
T Test ◽  
Blood Oxygen ◽  
Blood Oxygen Saturation ◽  
Temperature Changes ◽  
Cold Therapy ◽  
Arterial Blood ◽  
The Mean ◽  
Arterial Blood Oxygen

Background: Postoperative complications can endanger the patient's life and disrupt the recovery process if not properly managed. Local cold therapy can be a safe non-pharmacologic method to manage these side effects; however, it has not been highly considered. Objectives: This study was done to determine the effect of local cold therapy on arterial blood oxygen saturation and temperature changes in patients undergoing surgery. Methods: This quasi-experimental study was performed at Shahrekord University of Medical Sciences in 2019 on 60 patients undergoing thoracic and abdominal surgery selected by convenience sampling, and they were randomly assigned to the intervention and control groups. The intervention (local cold therapy) was performed for 48 hours after full consciousness, three times a day for 20 minutes to the intervention group. Data were collected before and after the intervention using a demographic questionnaire, pulse oximetry device, and thermometer. The data were analyzed based on the independent samples t-test, paired-samples t-test, and chi-square and Fishers’ exact tests by SPSS version 20 software. Results: The mean arterial blood oxygen saturation percentage (O2Sat%) during the intervention significantly increased in both groups (P < 0.05), but the rate of increase was significantly higher in the local cold group (P < 0.05). The results of within-group research showed that the mean temperature had significant differences in the local cold therapy group (P < 0.05), but the mean body temperature did not show a significant difference between the two groups (P < 0.05). Conclusions: Local cold therapy can be effectively trained and used by nurses to improve the O2Sat%. It did not affect postoperative body temperature. Further studies must be conducted to investigate the effects of local cold therapy on postoperative body temperature changes.

scholarly journals Effects of Bilateral Nasal Packing on Oxygen Saturation and Blood Pressure

2017 ◽  
Vol 56 (208) ◽  
pp. 442-6
Author(s):  
Meera Bista
Keyword(s):  
Blood Pressure ◽  
Oxygen Saturation ◽  
Significant Rise ◽  
Nasal Packing ◽  
P Value ◽  
Systemic Effects ◽  
Blood Oxygen ◽  
Blood Oxygen Saturation ◽  
Saturation Pulse ◽  
Randomized Sampling

Introduction: Nasal packing is one of the most common rhinological procedures in ENT practice exerting local in nose and systemic effects. Systemic effects may be due to poor sleep quality, respiratory difficulty, decreased oxygen saturation, circulatory problems and toxic shock syndrome which can threaten the life of a person.The study was done to investigate the effect on bilateral nasal packing on blood oxygen saturation and blood pressure changes. Methods: A cross-sectional prospective study was undertaken in Kathmandu Medical College from January 2017 to June 2017. The ethical approval was taken from institutional review committee of KMC and verbal consent was taken from each patient. Thirty patients were included in the study by simple randomized sampling. The association between nasal packing and oxygen saturation and blood pressure was done using ANNOVA and Fisher Exact test. P value of <0.005 was considered statistically significant. SPSS version 20 for windows OS was used for analysis. Results: Blood oxygen saturation on four different days was compared using ANNOVA and paired sample tests. It showed a significant dip on the day of bilateral nasal packing compared to other readings (P value <0.001). Likewise, systolic and diastolic blood pressures showed a significant rise on the day of bilateral nasal packing compared to readings on other days (P value <0.001). Conclusions: Bilateral nasal packing can lead to significant rise in systolic and diastolic blood pressure and a dip in blood oxygen saturation. Thus care has to be taken while performing bilateral nasal packing in normal patients but much more in high risk patients. Keywords:  blood pressure; nasal obstruction; nasal packing; oxygen saturation; pulse oximetry.

scholarly journals Anesthetic management of precise radiotherapy under apnea-like condition

2021 ◽  
Vol 49 (3) ◽  
pp. 030006052199026
Author(s):  
Shilong Zhang ◽  
Bin Zhao ◽  
Dongji Chen ◽  
Ying Qi ◽  
Youguo Ma ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Oxygen Saturation ◽  
Anesthetic Management ◽  
Lung Tumors ◽  
Blood Oxygen ◽  
Blood Oxygen Saturation ◽  
Pharyngeal Wall ◽  
Oxygen Saturation Level ◽  
Arterial Blood

Objective To study the safety and feasibility of implementation of precise radiotherapy with inducement of an apnea-like condition. Methods Two patients with lung tumors underwent precise radiotherapy under an apnea-like condition. The apnea-like condition was induced 11 times between the two patients for tumor localization and treatment. The changes in the blood oxygen saturation, blood pressure, heart rate, and end-tidal carbon dioxide during the apnea-like periods were observed, and the incidence of adverse reactions was recorded. Results The average apnea-like time was 6.2 minutes (range, 3–9 minutes), and the average radiotherapy time was 4.6 minutes (range, 1–7 minutes). The lowest blood oxygen saturation level was 97%, with a change of <1%. The heart rate and average arterial blood pressure increased during the apnea-like periods. Contact sores appeared on the patients’ posterior pharyngeal wall after the first apnea-like period; no other adverse events occurred. Conclusion Precise radiotherapy under an apnea-like condition is safe and feasible for patients with lung tumors.

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