scholarly journals Evaluation of a Pseudotyped Virus Neutralisation Test for the Measurement of Equine Influenza Virus-Neutralising Antibody Responses Induced by Vaccination and Infection

Vaccines ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 466
Author(s):  
Rebecca Kinsley ◽  
Stéphane Pronost ◽  
Manuelle De Bock ◽  
Nigel Temperton ◽  
Janet M. Daly ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Haemagglutination Inhibition ◽  
Antibody Responses ◽  
Pseudotyped Virus ◽  
Equine Influenza Virus ◽  
Serum Samples ◽  
Neutralising Antibodies ◽  
Protective Antibody ◽  
Equine Influenza ◽  
Antibody Assays

Equine influenza is a major respiratory disease of horses that is largely controlled by vaccination in some equine populations. Virus-neutralising antibodies, the mainstay of the protective immune response, are problematic in assaying for equine influenza virus, as most strains do not replicate efficiently in cell culture. Surrogate measures of protective antibody responses include the haemagglutination inhibition (HI) test and single radial haemolysis (SRH) assay. For this study, a pseudotyped virus, bearing an envelope containing the haemagglutinin (HA) from the Florida clade 2 equine influenza virus strain A/equine/Richmond/1/07 (H3N8), was generated to measure HA-specific neutralising antibodies in serum samples (n = 134) from vaccinated or experimentally-infected ponies using a pseudotyped virus neutralization test (PVNT). Overall, the results of PVNT were in good agreement with results from the SRH assay (100% sensitivity, 68.53% specificity) and HI test (99.2% sensitivity, 49.03% specificity). The PVNT was apparently more sensitive than either the SRH assay or the HI test, which could be advantageous for studying the antibody kinetics, particularly when antibody levels are low. Nevertheless, further studies are required to determine whether a protective antibody level can be defined for the SRH assay and to ascertain the inter-laboratory reproducibility. In conclusion, the PVNT efficiently measures neutralising antibodies after immunization and/or experimental infection in the natural host, and may complement existing antibody assays.

scholarly journals Development of an enzyme-linked immunosorbent assay (ELISA) for the detection of specific antibodies against an H7N7 and an H3N8 equine influenza virus

1984 ◽  
Vol 93 (3) ◽  
pp. 609-620 ◽  
Author(s):  
M. S. Denyer ◽  
J. R. Crowther ◽  
R. C. Wardley ◽  
R. Burrows
Keyword(s):  
Influenza Virus ◽  
Immunosorbent Assay ◽  
Solid Phase ◽  
Allantoic Fluid ◽  
Influenza Viruses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Equine Influenza Virus ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Equine Influenza

SummaryThis paper describes a solid-phase microtitre plate enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to equine influenza viruses. Using egg-grown influenza viruses as the antigens attached to the solid phase, crossreactions were observed between an H7N7 equine virus (designated A1) and an H3N8 equine influenza virus (designated A2) when untreated antisera were tested. Absorption of antisera with egg-grown A/Porcine/Shope/1/33 influenza virus eliminated cross-reactive antibodies so that specific detection of anti-equine influenza A1 or A2 antibodies was possible.Examination of horse sera following vaccination with A1 and/or A2 isolates showed that antibodies were produced against antigen associated with egg allantoic fluid as well as against virus. Such antibodies were eliminated following the absorption of antisera with porcine influenza virus. Results using sera from horses with known vaccination histories confirmed that the ELISA preferentially detected antibodies homologous to the antigen attached to the solid phase and methods to evaluate the current serological state of individual horses by relating the titres of specific antibodies against equine influenza A1 and A2 isolates are shown. This ELISA provides a simple and rapid method of assessing specific antibodies from horse sera and offers advantages over the ‘routine’ HI and SRH assessments since it gives high precision, is economical of reagents and has the capacity to handle large numbers of serum samples.

Formulation with CpG ODN enhances antibody responses to an equine influenza virus vaccine

2006 ◽  
Vol 114 (1-2) ◽  
pp. 103-110 ◽  
Author(s):  
A.M. Lopez ◽  
R. Hecker ◽  
G. Mutwiri ◽  
S. van Drunen Littel-van den Hurk ◽  
L.A. Babiuk ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Virus Vaccine ◽  
Influenza Virus Vaccine ◽  
Antibody Responses ◽  
Cpg Odn ◽  
Equine Influenza Virus ◽  
Equine Influenza

Antibody responses after vaccination against equine influenza virus in the Republic of Korea in 2019

2020 ◽  
Vol 44 (3) ◽  
pp. 132-135
Author(s):  
In-Ohk Ouh ◽  
Min Ji Kim ◽  
Hyeonhae Choi ◽  
Hyoung seok Yang ◽  
Sang Kyu Lee ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Antibody Responses ◽  
Republic Of Korea ◽  
Equine Influenza Virus ◽  
Equine Influenza ◽  
The Republic

scholarly journals Antibody Responses Induced by Japanese Whole Inactivated Vaccines against Equine Influenza Virus (H3N8) Belonging to Florida Sublineage Clade2

2011 ◽  
Vol 73 (4) ◽  
pp. 483-485 ◽  
Author(s):  
Takashi YAMANAKA ◽  
Hiroshi BANNAI ◽  
Manabu NEMOTO ◽  
Koji TSUJIMURA ◽  
Takashi KONDO ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Antibody Responses ◽  
Equine Influenza Virus ◽  
Equine Influenza ◽  
Inactivated Vaccines

Protein expression changes in cells inoculated with Equine Influenza Virus: antibody microarray analysis

2013 ◽  
Vol 16 (4) ◽  
pp. 663-669
Author(s):  
W. Rozek ◽  
M. Kwasnik ◽  
J.F. Zmudzinski
Keyword(s):  
Influenza Virus ◽  
Protein Quality ◽  
Biological Properties ◽  
Regulation Of Transcription ◽  
Equine Influenza Virus ◽  
Virus Antibody ◽  
Antibody Microarray ◽  
Equine Influenza ◽  
Microarray Technique ◽  
In Cells

AbstractChanges in the level of cellular proteins in cells inoculated with equine influenza virus H7N7 and H3N8 were studied with microarray technique. H3N8 induced pro-apoptotic proteins while H7N7 induced both pro- as well as anti-apoptotic factors. The higher level of some cytoskeleton components and proteins involved in the protein quality control was recorded. Relatively high number of proteins involved in the regulation of transcription was down-regulated. The pattern of changes observed for H7N7 and H3N8 may reflect differences in the biological properties of both serotypes.

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