Milk and milk products. Determination of soy and pea proteins using capillary electrophoresis in the presence of sodium dodecyl sulfate (SDS-CE). Screening method

2015 ◽  
Keyword(s):  
Capillary Electrophoresis ◽  
Sodium Dodecyl Sulfate ◽  
Screening Method ◽  
Sodium Dodecyl ◽  
Milk Products ◽  
Dodecyl Sulfate ◽  
Pea Proteins

scholarly journals Determination of Vegetal Proteins in Milk Powder by Sodium Dodecyl Sulfate–Capillary Gel Electrophoresis: Interlaboratory Study

2002 ◽  
Vol 85 (5) ◽  
pp. 1090-1095 ◽  
Author(s):  
María A Manso ◽  
Tiziana Maria Cattaneo ◽  
Stefania Barzaghi ◽  
Cornelis Olieman ◽  
Rosina López-Fandiño ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Total Protein ◽  
Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Milk Powder ◽  
Interlaboratory Study ◽  
Capillary Gel Electrophoresis ◽  
Dodecyl Sulfate ◽  
Pea Proteins

Abstract An interlaboratory study, with the participation of 8 laboratories, was conducted to evaluate a sodium dodecyl sulfate–capillary gel electrophoresis method for determination of adulteration of milk powder with soy and pea proteins. Calibration standards (0–8%, w/w, soy and pea protein in total protein) and adulterated skim milk powders (0–5%, w/w, soy and pea proteins in total protein) were produced. Vegetal proteins were determined after removal of milk proteins by pretreatment of the samples with tetraborate–EDTA buffer, pH 8.3. Repeatability standard deviations ranged from 9 to 15% and reproducibility standard deviations ranged from 25 to 30% in the samples containing 5% vegetal protein in total protein.

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