Soil quality. Identification of ecotoxicological test species by DNA barcoding

2020 ◽  
Keyword(s):  
Dna Barcoding ◽  
Soil Quality ◽  
Test Species ◽  
Ecotoxicological Test

Orthonychiurus pseudostachianus (collembola) as a toxicity test organism and selection of an ecotoxicological test battery to assess soil quality

2012 ◽  
Vol 54 ◽  
pp. 49-54 ◽  
Author(s):  
Lucia Santorufo ◽  
Rita Carotenuto ◽  
Annamaria Rocco ◽  
Fabiano De Luca Picione ◽  
Giulia Maisto
Keyword(s):  
Soil Quality ◽  
Toxicity Test ◽  
Test Organism ◽  
Test Battery ◽  
Ecotoxicological Test ◽  
Ecotoxicological Test Battery ◽  
Selection Of

DNA barcoding of earthworms (Eisenia fetida/andrei complex) from 28 ecotoxicological test laboratories

2016 ◽  
Vol 104 ◽  
pp. 3-11 ◽  
Author(s):  
Jörg Römbke ◽  
Manuel Aira ◽  
Thierry Backeljau ◽  
Karin Breugelmans ◽  
Jorge Domínguez ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Eisenia Fetida ◽  
Ecotoxicological Test ◽  
Eisenia Fetida Andrei

scholarly journals Unravelling taxonomic ambiguity of the Mastacembelidae in the Mekong Delta (Vietnam) through DNA barcoding and morphological approaches

2020 ◽  
Vol 33 (2) ◽  
Author(s):  
Thuy-Yen Duong ◽  
Liem Van Dung Tran ◽  
Ngoc-Tran Thi Nguyen ◽  
Jamsari Amirul Firdaus Jamaluddin ◽  
Mohd Nor Siti Azizah
Keyword(s):  
Dna Barcoding ◽  
Species Identification ◽  
Mekong Delta ◽  
The Body ◽  
Coi Gene ◽  
Sister Relationship ◽  
Test Species ◽  
Dorsal Fin ◽  
Mastacembelus Armatus ◽  
Fin Spines

Morphological-based species identification can be problematic for a comparative worldwide survey if taxonomic keys are limited and inconsistent, as illustrated in the family Mastacembelidae. This study combined DNA barcoding and morphological methods to test species identification of Mastacembelidae in the Mekong Delta with emphasis on taxonomic ambiguity of the precise identification of the fish locally known as chach bong. Fish specimens were collected from fishermen in different regions of the delta. Five presumed species within two genera were recorded. Samples were morphologically measured for morphometric and meristic traits. Representative samples of each species were sequenced at the cytochrome c oxidase subunit I (COI) gene. The number of dorsal fin spines and general morphological appearance are distinguishable among the five presumed species. However, morphometric measurements overlapped between Macrognathus semiocellatus and Macrognathus siamensis. K2P distances based on COI sequences among species were high, ranging from 12.4% to 18.7%. All individuals were separated into monophyletic groups of species, clustered into Mastacembelus and two Macrognathus lineages. Chach bong should be recognized as Mastacembelus favus and not Mastacembelus armatus as previously classified. No Mastacembelus armatus was recorded in the Mekong Delta. GenBank sequences of Mastacembelus armatus formed a sister relationship to Mastacembelus favus although both have the same range of number of dorsal fin spines and similar reticulated patterns on the body. Misidentification between these two species has been widely recorded in international databases of species taxonomy and DNA barcodes. Nonetheless, their genetic distance (12.4%) is higher than conspecific distances of samples from other regions, indicating the two species can be differentiated by DNA barcoding.

Effects of cadmium and chlordane on cellular apoptosis in ecotoxicological test species

2001 ◽  
Vol 81 (3-4) ◽  
pp. 153-160
Author(s):  
F. Z. Zeroual ◽  
M. Guerbet ◽  
G. Nguyen‐Ba ◽  
J. M. Jouany
Keyword(s):  
Test Species ◽  
Ecotoxicological Test ◽  
Cellular Apoptosis

Identification of the ecological requirements of important terrestrial ecotoxicological test species

2005 ◽  
Vol 13 (2) ◽  
pp. 51-83 ◽  
Author(s):  
S Jänsch ◽  
M J Amorim ◽  
J Römbke
Keyword(s):  
Organic Matter Content ◽  
Matter Content ◽  
Biological Assessment ◽  
Natural Soils ◽  
Test Species ◽  
Ecological Requirements ◽  
Ecotoxicological Test ◽  
Field Situation ◽  
Wide Range ◽  
Alternative Test

For about 20 years, standardized soil ecotoxicological tests have relied on the use of an artificial soil substrate (e.g., Organisation for Economic Co-operation and Development; OECD). For both the extrapolation of data obtained in the laboratory to the field situation, as well as the biological assessment of contaminated sites, this approach alone is not sufficient anymore. For this reason a literature review has been performed to investigate the ecological requirements of important terrestrial ecotoxicological test species. The invertebrate species included were Eisenia fetida, E. andrei (earthworms), Enchytraeus albidus, E. crypticus (potworms), Folsomia candida (springtails), and Hypoaspis aculeifer (predatory mites). The ecological parameters included were pH, moisture content, temperature, soil (i.e., texture, water-holding capacity, organic matter content, etc.), and food. The results indicate that most of these species should be applicable to a wide range of natural soils, while for some "extreme" soils (e.g., very acid forest soils) alternative test species will be required. Thus, further research is required to identify such species as well as to fill the gaps of knowledge concerning the ecological requirements of the species investigated here. Key words: Collembola, Enchytraeidae, Gamasid mites, Lumbricidae, natural soils.

DNA barcoding in fungi

AccessScience ◽  
2015 ◽  
Keyword(s):  
Dna Barcoding

DNA-Barcoding: the master key to authenticate medicinal plants?

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
J Novak
Keyword(s):  
Medicinal Plants ◽  
Dna Barcoding
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