Faculty Opinions recommendation of Nuclear targeting of adenovirus type 2 requires CRM1-mediated nuclear export.

2005 ◽  
Author(s):  
Greg Smith
Keyword(s):  
Nuclear Export ◽  
Adenovirus Type ◽  
Nuclear Targeting ◽  
Adenovirus Type 2

scholarly journals Nuclear Targeting of Adenovirus Type 2 Requires CRM1-mediated Nuclear Export

2005 ◽  
Vol 16 (6) ◽  
pp. 2999-3009 ◽  
Author(s):  
Sten Strunze ◽  
Lloyd C. Trotman ◽  
Karin Boucke ◽  
Urs F. Greber
Keyword(s):  
Nuclear Export ◽  
Adenovirus Type ◽  
Nuclear Pore ◽  
Nuclear Targeting ◽  
Microtubule Organizing Center ◽  
Cytoplasmic Transport ◽  
Viral Particles ◽  
Organizing Center ◽  
Adenovirus Type 2

Incoming adenovirus type 2 (Ad2) and Ad5 shuttle bidirectionally along microtubules, biased to the microtubule-organizing center by the dynein/dynactin motor complex. It is unknown how the particles reach the nuclear pore complex, where capsids disassemble and viral DNA enters the nucleus. Here, we identified a novel link between nuclear export and microtubule-mediated transport. Two distinct inhibitors of the nuclear export factor CRM1, leptomycin B (LMB) and ratjadone A (RJA) or CRM1-siRNAs blocked adenovirus infection, arrested cytoplasmic transport of viral particles at the microtubule-organizing center or in the cytoplasm and prevented capsid disassembly and nuclear import of the viral genome. In mitotic cells where CRM1 is in the cytoplasm, adenovirus particles were not associated with microtubules but upon LMB treatment, they enriched at the spindle poles implying that CRM1 inhibited microtubule association of adenovirus. We propose that CRM1, a nuclear factor exported by CRM1 or a protein complex containing CRM1 is part of a sensor mechanism triggering the unloading of the incoming adenovirus particles from microtubules proximal to the nucleus of interphase cells.

Uncoating of adenovirus type 2.

1979 ◽  
Vol 30 (2) ◽  
pp. 462-471 ◽  
Author(s):  
M A Mirza ◽  
J Weber
Keyword(s):  
Adenovirus Type ◽  
Adenovirus Type 2

Adenovirus type 2 DNA replication. II. Termini of DNA replication.

1975 ◽  
Vol 16 (4) ◽  
pp. 767-774 ◽  
Author(s):  
R Schilling ◽  
B Weingärtner ◽  
E L Winnacker
Keyword(s):  
Dna Replication ◽  
Adenovirus Type ◽  
Adenovirus Type 2

Termination sites for adenovirus type 2 DNA replication.

1975 ◽  
Vol 16 (4) ◽  
pp. 759-766 ◽  
Author(s):  
A Tolun ◽  
U Pettersson
Keyword(s):  
Dna Replication ◽  
Adenovirus Type ◽  
Adenovirus Type 2

Adenovirus type 2 VAI RNA transcription by polymerase III is blocked by sequence-specific methylation.

1991 ◽  
Vol 65 (4) ◽  
pp. 1735-1742 ◽  
Author(s):  
R Jüttermann ◽  
K Hosokawa ◽  
S Kochanek ◽  
W Doerfler
Keyword(s):  
Adenovirus Type ◽  
Rna Transcription ◽  
Polymerase Iii ◽  
Adenovirus Type 2
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