Faculty Opinions recommendation of Structural basis for osmotic regulation of the DNA binding properties of H-NS proteins.

2020 ◽  
Author(s):  
Charles Dorman
Keyword(s):  
Dna Binding ◽  
Structural Basis ◽  
Osmotic Regulation ◽  
Binding Properties ◽  
Dna Binding Properties

scholarly journals Structural basis for osmotic regulation of the DNA binding properties of H-NS proteins

2019 ◽  
Author(s):  
Liang Qin ◽  
Fredj Ben Bdira ◽  
Yann G. J. Sterckx ◽  
Alexander N. Volkov ◽  
Jocelyne Vreede ◽  
...  
Keyword(s):  
Dna Binding ◽  
Electrostatic Interactions ◽  
Intramolecular Interactions ◽  
Structural Basis ◽  
Binding Properties ◽  
Family Protein ◽  
Low Salt ◽  
Functional States ◽  
Mechanistic Basis ◽  
Dna Binding Properties

AbstractH-NS proteins act as osmotic sensors translating changes in osmolarity into altered DNA binding properties, thus, regulating enterobacterial genome organization and genes transcription. The molecular mechanism underlying the switching process and its conservation among H-NS family members remains elusive.Here, we focus on the H-NS family protein MvaT from P. aeruginosa and demonstrate experimentally that its protomer exists in two different conformations, corresponding to two different functional states. In the half-opened state (dominant at low salt) the protein forms filaments along DNA, in the fully opened state (dominant at high salt) the protein bridges DNA. This switching is a direct effect of ionic strengths on electrostatic interactions between the appositively charged DNA binding and N-terminal domains of MvaT. The asymmetric charge distribution and intramolecular interactions are conserved among the H-NS family of proteins. Therefore, our study establishes a general paradigm for the molecular mechanistic basis of the osmosensitivity of H-NS proteins.

scholarly journals Structural basis for osmotic regulation of the DNA binding properties of H-NS proteins

2020 ◽  
Vol 48 (4) ◽  
pp. 2156-2172 ◽  
Author(s):  
Liang Qin ◽  
Fredj Ben Bdira ◽  
Yann G J Sterckx ◽  
Alexander N Volkov ◽  
Jocelyne Vreede ◽  
...  
Keyword(s):  
Dna Binding ◽  
Electrostatic Interactions ◽  
Intramolecular Interactions ◽  
Structural Basis ◽  
Binding Properties ◽  
Family Protein ◽  
Low Salt ◽  
Mechanistic Basis ◽  
Oppositely Charged ◽  
Dna Binding Properties

Abstract H-NS proteins act as osmotic sensors translating changes in osmolarity into altered DNA binding properties, thus, regulating enterobacterial genome organization and genes transcription. The molecular mechanism underlying the switching process and its conservation among H-NS family members remains elusive. Here, we focus on the H-NS family protein MvaT from Pseudomonas aeruginosa and demonstrate experimentally that its protomer exists in two different conformations, corresponding to two different functional states. In the half-opened state (dominant at low salt) the protein forms filaments along DNA, in the fully opened state (dominant at high salt) the protein bridges DNA. This switching is a direct effect of ionic strength on electrostatic interactions between the oppositely charged DNA binding and N-terminal domains of MvaT. The asymmetric charge distribution and intramolecular interactions are conserved among the H-NS family of proteins. Therefore, our study establishes a general paradigm for the molecular mechanistic basis of the osmosensitivity of H-NS proteins.

scholarly journals Purification, subunit structure, and DNA binding properties of the mouse oxysterol receptor

1989 ◽  
Vol 264 (31) ◽  
pp. 18433-18439 ◽  
Author(s):  
F R Taylor ◽  
E P Shown ◽  
E B Thompson ◽  
A A Kandutsch
Keyword(s):  
Dna Binding ◽  
Subunit Structure ◽  
Binding Properties ◽  
Dna Binding Properties

The DNA binding properties of 9-aminoacridine carboxamide Pt complexes

2021 ◽  
pp. 116191
Author(s):  
Hieronimus W. Kava ◽  
Wai Y. Leung ◽  
Anne M. Galea ◽  
Vincent Murray
Keyword(s):  
Dna Binding ◽  
Binding Properties ◽  
Dna Binding Properties

scholarly journals DNA-binding properties of a lac repressor mutant incapable of forming tetramers.

1991 ◽  
Vol 266 (2) ◽  
pp. 1281-1288 ◽  
Author(s):  
M Brenowitz ◽  
N Mandal ◽  
A Pickar ◽  
E Jamison ◽  
S Adhya
Keyword(s):  
Dna Binding ◽  
Lac Repressor ◽  
Binding Properties ◽  
Dna Binding Properties
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