scholarly journals THE EFFECT OF N-ACETYL-L-CYSTEINE ON BIOFILM OF VIBRIO CHOLERAE

2018 ◽  
pp. 83-87 ◽  
Author(s):  
O. V. Duvanova ◽  
B. N. Mishankin ◽  
S. V. Titova ◽  
L. A. Korneeva
Keyword(s):  
Antibacterial Activity ◽  
Drug Therapy ◽  
Vibrio Cholerae ◽  
Antibacterial Effect ◽  
Antibacterial Action ◽  
Causative Agents ◽  
Planktonic Form ◽  
El Tor ◽  
Epidemiological Significance

Aim. To study the effect of N-acetyl-L-cysteine on biofilm of V. cholerae of different sero-groups isolated from various sources and with different epidemiological significance (the presence/ absence of the ctx AB genes and tcpA). Materials and methods. Bacterial eiiltiire of Vibrio cholerae El Tor O1 and O139 serogroups were grown as biolms. We have estimated the influence of the drug N-acetyl-L-cysteine at a concentration of 0.5 - 4 mg/ml on the formation, of the formed biofilm and in planktonic form. Results. Discovered antibacterial activity of N-acetyl-L-cysteine. Noted that it was influenced as in the formation, of the already formed biofilm and the planktonic form, the representatives of the Vibrio cholerae El Tor O1 and O139 serogroups in concentrations of 2 - 4 mg/ml, showing an antibacterial effect regardless of the presence/absence of genes ctx and tcpA AB. Conclusion. Identified the antibacterial action of the drug N-acetyl-L-cysteine against biofilm of V. cholerae indicates the desirability of considering the possibility of using drug therapy in cases variety of diseases caused by causative agents II - IV groups pathogenicity.

Genome structure and origin of nontoxigenic strains of Vibrio cholerae of El Tor biovar with different epidemiological significance

2016 ◽  
Vol 52 (9) ◽  
pp. 914-925 ◽  
Author(s):  
N. I. Smirnova ◽  
T. A. Kul’shan’ ◽  
E. Yu. Baranikhina ◽  
Ya. M. Krasnov ◽  
D. A. Agafonov ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Genome Structure ◽  
El Tor ◽  
Epidemiological Significance

scholarly journals A Quinazoline-2,4-Diamino Analog Suppresses Vibrio cholerae Flagellar Motility by Interacting with Motor Protein PomB and Induces Envelope Stress

2013 ◽  
Vol 57 (8) ◽  
pp. 3950-3959 ◽  
Author(s):  
Hongxia Wang ◽  
Li Zhang ◽  
Anisia J. Silva ◽  
Jorge A. Benitez
Keyword(s):  
Cholera Toxin ◽  
Vibrio Cholerae ◽  
Motor Protein ◽  
Polar Flagellum ◽  
Flagellar Motility ◽  
Extracellular Rna ◽  
Content Type ◽  
Envelope Stress ◽  
Causative Agents ◽  
El Tor

ABSTRACTVibrio choleraestrains of serogroups O1 and O139, the causative agents of the diarrheal illness cholera, express a single polar flagellum powered by sodium motive force and require motility to colonize and spread along the small intestine. In a previous study, we described a high-throughput assay for screening for small molecules that selectively inhibit bacterial motility and identified a family of quinazoline-2,4-diamino analogs (Q24DAs) that (i) paralyzed the sodium-driven polar flagellum ofVibriosand (ii) diminished cholera toxin secreted by El Tor biotypeV. cholerae. In this study, we provide evidence that a Q24DA paralyzes the polar flagellum by interacting with the motor protein PomB. Inhibition of motility with the Q24DA enhanced the transcription of the cholera toxin genes in both biotypes. We also show that the Q24DA interacts with outer membrane protein OmpU and other porins to induce envelope stress and expression of the extracellular RNA polymerase sigma factor σE. We suggest that Q24DA-induced envelope stress could affect the correct folding, assembly, and secretion of pentameric cholera toxin in El Tor biotypeV. choleraeindependently of its effect on motility.

scholarly journals ESTIMATION OF ANTIBACTERIAL EFFECT ON VIBRIO CHOLERAE (ON THE EXAMPLE OF THE SUBSTANCE ACETYL-N- CYSTEINE–L)

2019 ◽  
pp. 55-57
Author(s):  
O.V. Duvanova ◽  
B.N. Mishan′kin
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Carbon Source ◽  
Vibrio Cholerae ◽  
Sole Carbon Source ◽  
Inhibitory Concentration ◽  
Antibacterial Effect ◽  
Synthetic Medium ◽  
Nutrient Media ◽  
Cholera Vibrio ◽  
El Tor

We studied the effect of the substance acetyl-N-cysteine-L on cholera Vibrio. Its minimum inhibitory concentration for the strains of Vibrio cholerae El Tor O1 and O139 serogroup was 1–2.5 mg/ml in solid nutrient media - Martin's agar (pH 7.6) and LB (pH 7.2), while in Bhascaran synthetic medium, using glucose (0.1per cent) as the sole carbon source, N-acetyl-β-D-glucosamine (0.05 per cent) or colloidal chitin (0.027 per cent) it was reduced to 50–250 μg/ml. The ability of the substance acetyl-N-cysteine-L substance to suppress the activity of the purified enzyme N-acetyl-β-D-glucosaminidase (chitobiasis) was found. Antibacterial effect detected of the substance acetyl-N-cysteine-L against the strains of Vibrio cholerae El Tor O1 and O139 serogroups with different epidemic significance (presence / absence of ctxAB and tcpA genes) indicates the advisability of considering the issue on the possibility of including this substance in composition of solution components used in the rehydration therapy of diarrheal diseases cases.

scholarly journals EVALUATION OF NUTRIENT MEDIA TO GROW SOME INFECTION DISEASES CAUSATIVE AGENTS

2017 ◽  
pp. 104-110
Author(s):  
D. I. Kaminsky ◽  
V. V. Lobanov ◽  
K. K. Rozhkov ◽  
A. B. Mazrukho
Keyword(s):  
Yersinia Pestis ◽  
Vibrio Cholerae ◽  
Gold Standard ◽  
Detection Methods ◽  
Nutrient Media ◽  
Metabolic Plasticity ◽  
Causative Agents ◽  
Microbial Agents ◽  
Epidemiological Significance ◽  
Physiological Peculiarity

The detection methods for microbial agents that have epidemiological significance are diversity but cultivation on nutritional media remains the gold standard in microbiological diagnostics. Choice of medium depends on the conditions in which, bacteria were early and is present. The nature life determines its physiological peculiarity then a metabolic plasticity promote to survive and to save the virulence. In. this review on the example of Yersinia pestis and Vibrio cholerae performed evaluations of the efficient decisions for the bacterial media development. It is declared advantage of baker’s yeast hydrolisate as the nutrition media base.

scholarly journals RETROSPECTIVE ANALYSIS OF BIOLOGICAL AND MOLECULAR-GENETIC PROPERTIES OF STRAINS - CAUSATIVE AGENTS OF CHOLERA - ISOLATED IN UKRAINE IN 1994 - 2011

2017 ◽  
pp. 49-55
Author(s):  
I. V. Savelieva ◽  
S. N. Tikhonov ◽  
V. N. Saveliev ◽  
D. A. Kovalev ◽  
S. V. Pisarenko ◽  
...  
Keyword(s):  
Retrospective Analysis ◽  
Vibrio Cholerae ◽  
Molecular Genetic ◽  
Test System ◽  
Personal Genome ◽  
International Database ◽  
Cholera Vibrio ◽  
Causative Agents ◽  
Pcr Method ◽  
El Tor

Aim. Retrospective analysis of biological and molecular-genetic properties of strains - causative agents of cholera - isolated in the period of epidemics in Ukraine in 1994 - 2011. Materials and methods. Phenotypic and molecular-genetic properties of 5 strains of cholera vibrios, biovar El Tor isolated from cholera patients and 4 strains from the environmental samples were studied using traditional bacteriological and genetic methods. Detection of DNA for toxigenicity genes and genes characteristic for El Tor and classic biovar were carried out by PCR method using reagent kits «AmpliSens- Vibrio cholerae FRT» and «.Vibrio cholerae ctxB-rstR-rstC genes, REF» (an experimental test system). Sequencing of genomes of 4 strains of causative agents of cholera was carried out in genetic analyzer Ion Torrent Personal Genome Machine. Results. Strains of cholera vibrios identified in Ukraine in 1994 and 2011 such as a typical toxigenic biovar El Tor (V cholerae 01, El Tor, Ogawa, Hly-, ctxA+, tcpA+) contain genes of the classic cholera vibrio in their genome and are genetically altered (hybrid) variants of cholera vibrio biovar El Tor producing enterotoxin CT1 and having increased virulence, that was clinically manifested in predominance of severe forms of cholera in Mariupol of Donetsk region in 2011. Genome sequences of the 4 studied strains were deposited into the international database DDBJ/EMBL/GenBank. Conclusion. The studied isolates were established to belong to a clade of strains associated with cholera outbreaks in Haiti and Asian continent, from where genetically altered strains of cholera vibrios biovar El Tor were introduced to Haiti in 2010, based on results of comparison of genomic sequences of the studied strains with genomes of V. cholera strains from the international database GenBank.

Antibacterial Activity of Biodegradable Particles based on Chitosan Containing Colloidal Silver

2020 ◽  
Vol 36 (4) ◽  
pp. 87-93
Author(s):  
V.Yu. Reshetova ◽  
A.F. Krivoshchepov ◽  
I.A. Butorova ◽  
N.B. Feldman ◽  
S.V. Lutsenko ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Polymer Matrix ◽  
Adipic Acid ◽  
Antibacterial Effect ◽  
Academic Excellence ◽  
Colloidal Silver ◽  
Scanning Electron Microscopy Data ◽  
Covalent Bonds ◽  
Agar Diffusion Test

Chitosan beads with colloidal silver nanoparticles inclued in the polymer matrix have been obtained by the introduction of chitosan into an acidified nanosilver sol. Dual interconnection of drops of the resulting solution was then carried out by ionotropic gelation at the first stage and covalent crosslinking of the polymer matrix with adipic acid at the second stage. The surface morphology of the obtained beads was studied by scanning electron microscopy. Data of Fourier transform IR spectroscopy confirmed the formation of covalent bonds between chitosan and adipic acid. The antibacterial activity of obtained beads against S. aureus and E. coli was evaluated using agar diffusion test. It was shown that the сhitosan beads modified with nanostructured silver exhibited an antibacterial effect against the tested strains, and they can be used as a basis for creating biodegradable wound healing dressings with a prolonged antibacterial effect. chitosan, silver nanoparticles, antibacterial activity, wound dressings This work was supported by the "Russian Academic Excellence Project 5-100". The study was carried out with the financial support of the Russian Foundation for Basic Research in the framework of the Scientific Project no. 18-29-18039.

Genomic Variability of Pathogenicity Islands in Nontoxigenic Strains of Vibrio cholerae O1 Biotype El Tor

2020 ◽  
Vol 56 (9) ◽  
pp. 1055-1069
Author(s):  
N. I. Smirnova ◽  
A. A. Kritsky ◽  
J. V. Alkhova ◽  
E. Yu. Agafonova ◽  
E. Yu. Shchelkanova ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Pathogenicity Islands ◽  
Genomic Variability ◽  
Vibrio Cholerae O1 ◽  
El Tor

RESISTANT VIBRIO CHOLERÆ EL TOR IN TANZANIA

The Lancet ◽  
1979 ◽  
Vol 314 (8134) ◽  
pp. 147-148 ◽  
Author(s):  
K.J. Towner ◽  
N.J. Pearson ◽  
F. O'Grady
Keyword(s):  
Vibrio Cholerae ◽  
El Tor

Vibrio cholerae O1 El Tor variant and emergence of Haitian ctxB variant in the strains isolated from South India

2015 ◽  
Vol 205 (2) ◽  
pp. 195-200 ◽  
Author(s):  
Debdutta Bhattacharya ◽  
Shuchismita Dey ◽  
Gururaja Perumal Pazhani ◽  
Thandavarayan Ramamurthy ◽  
Mahantesh V. Parande ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
South India ◽  
Vibrio Cholerae O1 ◽  
El Tor

scholarly journals Isolation of Vibrio cholerae O1 strains similar to pre-seventh pandemic El Tor strains during an outbreak of gastrointestinal disease in an island resort in Fiji

2006 ◽  
Vol 55 (11) ◽  
pp. 1559-1562 ◽  
Author(s):  
G. Balakrish Nair ◽  
Ashrafus Safa ◽  
N. A. Bhuiyan ◽  
Suraia Nusrin ◽  
Denise Murphy ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Gastrointestinal Disease ◽  
Vibrio Cholerae O1 ◽  
El Tor
Sign in / Sign up

Export Citation Format

Share Document