Current strategies of allergen-specific immunotherapy

The article discusses the principles of the optimal allergovaccine choice for allergen-specific immunotherapy (ASIT) for grass-pollen-allergy patients; reviewed efficacy and safety studies of the standardized mixt five-grass-pollen extract. Relatively grass sensitization is preferred use mixt grass-pollen extract is thus more suitable for specific immunotherapy, as it contains a broader repertoire of the epitopes, which approximate to the natural exposure conditions.

Download Full-text

Effect of allergen-specific immunotherapy on platelet secretory activity in patients with grass-pollen allergy

Vaccine ◽

10.1016/j.vaccine.2006.04.043 ◽

2006 ◽

Vol 24 (47-48) ◽

pp. 6990-6993 ◽

Cited By ~ 6

Author(s):

Alicja Kasperska-Zajac ◽

Zenon Brzoza ◽

Barbara Rogala

Keyword(s):

Grass Pollen ◽

Specific Immunotherapy ◽

Pollen Allergy ◽

Secretory Activity ◽

Grass Pollen Allergy ◽

Allergen Specific Immunotherapy

Download Full-text

A hypoallergenic hybrid molecule with increased immunogenicity consisting of derivatives of the major grass pollen allergens, Phl p 2 and Phl p 6

Biological Chemistry ◽

10.1515/bc.2008.105 ◽

2008 ◽

Vol 389 (7) ◽

Cited By ~ 24

Author(s):

Birgit Linhart ◽

Nadine Mothes-Luksch ◽

Susanne Vrtala ◽

Michael Kneidinger ◽

Peter Valent ◽

...

Keyword(s):

Grass Pollen ◽

Specific Immunotherapy ◽

Pollen Allergy ◽

Poor Quality ◽

Hybrid Molecule ◽

Grass Pollen Allergy ◽

Allergen Specific Immunotherapy ◽

Ige Reactivity ◽

Allergenic Activity ◽

Allergen Extracts

Abstract Allergen-specific immunotherapy is currently based on the administration of allergen extracts containing natural allergens. However, its broad application is limited by the poor quality of these extracts. Based on recombinant allergens, well-defined allergy vaccines for allergen-specific immunotherapy can be produced. Furthermore, they can be modified to reduce their allergenic activity and to avoid IgE-mediated side effects. Here, we demonstrate that the immunogenicity of two grass pollen-derived hypoallergenic allergen derivatives could be increased by engineering them as a single hybrid molecule. We used a hypoallergenic Phl p 2 mosaic, generated by fragmentation of the Phl p 2 sequence and reassembly of the resulting peptides in an altered order, and a truncated Phl p 6 allergen, to produce a hybrid protein. The hybrid retained the reduction of IgE reactivity and allergenic activity of its components as shown by ELISA and basophil activation assays. Immunization with the hybrid molecule demonstrated the increased immunogenicity of this molecule, leading to higher levels of allergen-specific IgG antibodies compared to the single components. These antibodies could inhibit patients' IgE binding to the wild-type allergens. Thus, the described strategy allows the development of safer and more efficacious vaccines for the treatment of grass pollen allergy.

Download Full-text

Faculty Opinions recommendation of Molecular profiles of IgE to Phleum pratense in children with grass pollen allergy: implications for specific immunotherapy.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.13492000.14870106 ◽

2012 ◽

Author(s):

Giorgio Walter Canonica ◽

Enrico Compalati

Keyword(s):

Grass Pollen ◽

Specific Immunotherapy ◽

Pollen Allergy ◽

Phleum Pratense ◽

Grass Pollen Allergy ◽

Molecular Profiles

Download Full-text

Specific immunotherapy in grass pollen allergy

Human Vaccines & Immunotherapeutics ◽

10.4161/hv.22357 ◽

2012 ◽

Vol 8 (10) ◽

pp. 1544-1547 ◽

Cited By ~ 3

Author(s):

Claire Mailhol ◽

Alain Didier

Keyword(s):

Grass Pollen ◽

Specific Immunotherapy ◽

Pollen Allergy ◽

Grass Pollen Allergy

Download Full-text

The importance of cross-reactivity in grass pollen allergy

Archives of Biological Sciences ◽

10.2298/abs1403149a ◽

2014 ◽

Vol 66 (3) ◽

pp. 1149-1155 ◽

Cited By ~ 1

Author(s):

Ivana Aleksic ◽

Olga Vuckovic ◽

Katarina Smiljanic ◽

Marija Gavrovic-Jankulovic ◽

Vera Krsmanovic ◽

...

Keyword(s):

Grass Pollen ◽

Pollen Allergy ◽

Immunoblot Analysis ◽

Cross Reactivity ◽

Phleum Pratense ◽

Pollen Extract ◽

Grass Pollen Allergy ◽

Number Of Patients

According to the data obtained from in vivo and in vitro testing in Serbia, a significant number of patients have allergic symptoms caused by grass pollen. We examined the protein composition of grass pollens (Dactylis glomerata, Lolium perenne and Phleum pratense) and cross-reactivity in patients allergic to grass pollen from our region. The grass pollen allergen extract was characterized by SDS-PAGE, while cross-reactivity of single grass pollens was revealed by immunoblot analysis. A high degree of cross-reactivity was demonstrated for all three single pollens in the sera of allergic patients compared to the grass pollen extract mixture. Confirmation of the existence of cross-reactivity between different antigenic sources facilitates the use of monovalent vaccines, which are easier to standardize and at the same time prevent further sensitization of patients and reduces adverse reactions.

Download Full-text