Rapid detection of sulfamethazine and ofloxacin residues in chicken based on synchronous fluorescence spectrum

2021 ◽  
Vol 29 (4) ◽  
pp. 721-732
Author(s):  
Jian CHEN ◽  
◽  
Mu-hua LIU ◽  
Hai-chao YUAN ◽  
Shuang-gen HUANG ◽  
...  
Keyword(s):  
Fluorescence Spectrum ◽  
Rapid Detection ◽  
Synchronous Fluorescence ◽  
Synchronous Fluorescence Spectrum

Interference of SDS on Synchronous Fluorescence Spectrum of SDBS Reduced by HP-β-CD

2017 ◽  
Vol 38 (4) ◽  
pp. 535-542
Author(s):  
石东坡 SHI Dong-po ◽  
尹先清 YIN Xian-qing ◽  
陈 武 CHEN Wu ◽  
郑延成 ZHENG Yan-cheng ◽  
付家新 FU Jia-xin ◽  
...  
Keyword(s):  
Fluorescence Spectrum ◽  
Synchronous Fluorescence ◽  
Synchronous Fluorescence Spectrum

Studies on the Interaction between Imidacloprid and Bovine Serum Albumin by Spectrometry

2013 ◽  
Vol 726-731 ◽  
pp. 199-203
Author(s):  
Rui Xin Guo ◽  
Zhi Liang Wang ◽  
Zhi Jun Hu ◽  
Guo Ling Li ◽  
Jian Qiu Chen
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Fluorescence Spectrum ◽  
Bovine Serum ◽  
Synchronous Fluorescence ◽  
Binding Studies ◽  
Single Class ◽  
Physiological Conditions ◽  
Synchronous Fluorescence Spectrometry ◽  
Hoff Equation

The binding studies of imidacloprid to bovine serum albumin (BSA) were investigated by UV-Vis absorption spectrum, fluorescence spectrum and synchronous fluorescence spectrometry. Under the simulative physiological conditions, fluorescence data revealed the presence of a single class of binding site on BSA and the dynamic quenching constants () were 6.851×104 L.mol-1 and 5.813×104 L.mol-1 at 310 and 315 K, respectively, proving the mode of action of imidacloprid with BSA as a static quenching. In addition, according to the Vant Hoff equation, ΔGθ <0 showed="" the="" combination="" of="" imidacloprid="" and="" bsa="" was="" a="" spontaneous="" process="" h="" sup="">θ <0 and="" s="" sup="">θ> 0, indicated an electrostatic interaction process. At the same time, synchronous fluorescence spectrum of BSA could tell us whether the conformation of BSA was changed by imidacloprid.

scholarly journals In situ detection of live-to-dead bacteria ratio after inactivation by means of synchronous fluorescence and PCA

2018 ◽  
Vol 115 (4) ◽  
pp. 668-673 ◽  
Author(s):  
Runze Li ◽  
Umang Goswami ◽  
Maria King ◽  
Jie Chen ◽  
Thomas C. Cesario ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Rapid Detection ◽  
Fluorescence Spectra ◽  
Uv Light ◽  
Principal Component ◽  
Synchronous Fluorescence ◽  
Health Care Associated Infection ◽  
In Situ Detection

The determination of live and dead bacteria is of considerable significance for preventing health care-associated infection in hospitals, field clinics, and other areas. In this study, the viable (live) and nonviable (dead) bacteria in a sample were determined by means of their fluorescence spectra and principal component analysis (PCA). Data obtained in this study show that it is possible to identify bacteria strains and determine the live/dead ratio after UV light inactivation and antibiotic treatment, in situ, within minutes. In addition, synchronous fluorescence scans enable the identification of bacterial components such as tryptophan, tyrosine, and DNA. Compared with the time-consuming plating and culturing methods, this study renders a means for rapid detection and determination of live and dead bacteria.

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