Cloning and Relative Quantitative Determination of Trypsin-like Enzyme and Chymotrypsin-like Enzyme Genes in Larval Mid-gut of Bt Susceptible and ResistantHelicoverpa armigera(Hübner)*

2010 ◽  
Vol 2009 (5) ◽  
pp. 630-633
Author(s):  
Yanxia SHI ◽  
Yongjun ZHANG ◽  
Baofeng DOU ◽  
Gemei LIANG ◽  
Jiguo GAO ◽  
...  
RSC Advances ◽  
2015 ◽  
Vol 5 (125) ◽  
pp. 103474-103479 ◽  
Author(s):  
Lingling Wang ◽  
Yuting Nie ◽  
Yujiao Wang ◽  
Zhenyu Wang ◽  
Bo Xiong

Qualitative and quantitative SPE followed by HPLC-TOF/MS determination of pyridalyl and its potential metabolites in the excrement of Helicoverpa armigera (H. armigera) and Spodoptera exigua (S. exigua) was developed.


2020 ◽  
Vol 12 (3) ◽  
pp. 109
Author(s):  
NURINDAH NURINDAH ◽  
DWI ADI SUNARTO

ABSTRAK<br />Penelitian ini bertujuan untuk mengevaluasi efektivitas beberapa<br />predator terhadap H. armigera pada kapas. Penelitian dilakukan pada<br />pertanaman kapas di daerah pengembangan Lamongan, Jawa Tmur.<br />Kegiatan penelitian terdiri atas dua kegiatan, yaitu penentuan faktor<br />mortalitas biotik H. armigera dan perkembangan populasi H. armigera<br />pada lahan tanpa penyemprotan insektisida. Kegiatan penentuan faktor<br />mortalitas biotik H. armigera dilakukan pada 5 lahan tanpa penyemprotan<br />insektisida masing-masing seluas 100 m 2 dengan mengamati populasi H.<br />armigera dan predatornya. Kegiatan perkembangan populasi H. armigera<br />pada lahan tanpa penyemprotan insektisida dilakukan pada lahan seluas 5<br />hektar dengan mengamati populasi serangga hama, predator, dan hasil<br />kapas berbiji pada setiap sub petak yang luasnya 0,2 ha. Hasil penelitian<br />menunjukkan bahwa kompleks predator yang terdiri dari kumbang kubah,<br />semut, kepik mirid dan laba-laba berperan dalam pengaturan populasi H.<br />armigera. Kumbang kubah dan semut merupakan faktor mortalitas yang<br />penting bagi telur H. armigera, sedangkan kompleks predator merupakan<br />faktor mortalitas yang penting bagi larva kecil H. armigera. Persamaan<br />regresi hubungan antara kompleks predator dan larva H. armigera adalah<br />y= 3796 – 0.431x, sehingga dapat diartikan bahwa satu larva H. armigera<br />dapat dieliminasi oleh 8 ekor predator. Jika keberadaan predator<br />dipertimbangkan dalam ambang kendali H. armigera, maka pada sistem<br />tanam kapas tumpangsari dengan kedelai, populasi H. armigera tidak<br />pernah mencapai ambang kendali. Produksi kapas berbiji pada lahan tanpa<br />penyemprotan insektisida adalah 911 kg/ha; hasil ini jauh lebih tinggi<br />dibandingkan dengan produksi dari lahan dengan pengendalian secara<br />konvensional (penyemprotan insektisida secara intensif) yang hanya<br />mencapai 312 kg/ha. Oleh karena itu, keberadaan predator perlu<br />dipertimbangkan dalam konsep ambang kendali H. armigera.<br />Kata kunci : Kapas, Gossypium hirsutum L., Helicoverpa armigera,<br />predator, ambang kendali, produksi, Jawa Timur<br />ABSTRACT<br />Effectiveness of predators on Helicoverpa armigera on<br />cotton intercropped with soybean<br />Research on effectiveness of predators of H. amigera on cotton<br />intercropped with soybean was conducted in Lamongan, East Java. The<br />research activity included determination of the biotic mortality factor of H.<br />armigera and observation on H. armigera population in unsprayed cotton<br />field. Determination of the biotic mortality factor of H. armigera was<br />conducted in 5 unsprayed plots @ 100 m 2 by recording the number of H.<br />armigera and its predators. Observation on H. armigera population was<br />done in 5 hectares of unsprayed cotton field by observing the population of<br />H. armigera and its predators as well as cotton seed production of each 0.2<br />hectare sub plot. The research results showed that predator complex<br />(Coccinellid beetles, mirid bugs, ants and spiders) have significant role in<br />regulating H. armigera population. Coccinellid beetles and ants were the<br />important biotic mortality factors of H. armigera eggs, while complex<br />predators were that of the small larvae. Regression equation of predator<br />complex and H. armigera larvae was y= 3.976 – 0.43x, meaning that one<br />H. armigera larvae could be eliminated by 8 predators. If the presence of<br />predators was considered in action threshold concept, population of H.<br />armigera on cotton intercropped with soybean was always under action<br />threshold. Cotton seed production of unsprayed field was 911 kg/ha; this<br />was significantly higher than that of conventional control system with<br />intensive sprays of chemical insecticides. Therefore, the presence of<br />predators should be included in action threshold concept of H. armigera.<br />Key words : Cotton, Gossypium hirsutum L., pest, Helicoverpa armigera,<br />predator, action threshold, production, East Java


1970 ◽  
Vol 33 (4) ◽  
pp. 555-563 ◽  
Author(s):  
MA Zahid ◽  
MM Islam ◽  
MH Reza ◽  
MHZ Prodhan ◽  
M Rumana Begum

Chickpea pod borer Helicoverpa armigera (Hubner) is considered to be one of the major pests of chickpea. The damage potential and economic threshold level for Helicoverpa armigera larvae on chickpea crop were worked out. On an average, single larva per m row reduced the yield to the extent of 155 kg/ha and 157 kg/ha in 2004-05 and 2005-06 cropping season, respectively. The ratio of the value of yield saved to the cost of insecticide application at one larva per m row was 1.06 and 1.12 in 2004-05 and 2005-06, respectively. EILs for Helicoverpa armigera were determined as 12 and 0.95 per m row and ETLs was at 09 and 0 73 larvae per m row in 2004-05 and 2005 - 06, respectively. Key Words: Economic injury, chickpea, pod borer doi: 10.3329/bjar.v33i4.2288 Bangladesh J. Agril. Res. 33(4) : 555-563, December 2008


2020 ◽  
Vol 101 (12) ◽  
pp. 1300-1304
Author(s):  
Thrishantha Munsamy ◽  
Gustav Bouwer

Determination of the virulence of occlusion bodies (OBs), which are the horizontal transmission structures of nucleopolyhedroviruses (NPVs), is an important area of baculovirology. A method for inoculating an insect with an isolated OB was developed using Helicoverpa armigera nucleopolyhedrovirus (HearNPV) infection of second instar Helicoverpa armigera larvae as a model NPV–host pathosystem. In this novel method, laser capture microdissection (LCM) was used to directly catapult single OBs onto the surface of insect diet in bioassay containers. Since exposure via the natural oral horizontal transmission route of each larva to a single OB was established and not subject to chance variation, the method facilitated determination of the insect mortality rate (4.8%) associated with exposure to single HearNPV OBs. Droplet feeding bioassays confirmed that the novel method did not reduce OB virulence. The LCM method sets a foundation for virulence and genetic diversity studies based on single NPV OBs.


1999 ◽  
Vol 96 (9/10) ◽  
pp. 1608-1615
Author(s):  
T. E. Malliavin ◽  
H. Desvaux ◽  
M. A. Delsuc

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