scholarly journals Ephrin-As Cooperate With EphA4 to Promote Trunk Neural Crest Migration

2002 ◽  
Vol 10 (5) ◽  
pp. 295-305 ◽  
Author(s):  
R. Mclennan ◽  
C.E. Krull
Keyword(s):  
Neural Crest ◽  
Trunk Neural Crest ◽  
Neural Crest Migration

An SEM analysis of neural crest migration in the mouse

Development ◽  
1983 ◽  
Vol 74 (1) ◽  
pp. 97-118
Author(s):  
C. A. Erickson ◽  
J. A. Weston
Keyword(s):  
Neural Crest ◽  
Basal Lamina ◽  
Neural Tube ◽  
Neural Crest Cells ◽  
Sem Analysis ◽  
Basement Membranes ◽  
Dorsal Neural Tube ◽  
Trunk Neural Crest ◽  
Unique Cell ◽  
Neural Crest Migration

The cellular morphology and migratory pathways of the trunk neural crest are described in normal mouse embryos, and in embryos homozygous for Patch in which neural crest derivatives develop abnormally. Trunk neural crest cells initially appear in 8½-day embryos as a unique cell population on the dorsal neural tube surface and are relatively rounded. Once they begin to migrate the cells flatten and orient somewhat tangentially to the neural tube, and advance ventrad between the somites and neural tube. At the onset of migration neural crest cells extend lamellipodia onto the surface of the tube while detaching their trailing processes from the lumenal surface. The basal lamina on the dorsal neural tube is discontinuous when cell migration begins in this region. As development proceeds, the basal lamina gradually becomes continuous from a lateral to dorsal direction and neural crest emigration is progressively confined to the narrowing region of discontinuous basal lamina. Cell separation from the neural tube ceases concomitant with completion of a continuous basement membrane. Preliminary observations of the mutant embryos reveal that abnormal extracellular spaces appear and patterns of crest migration are subsequently altered. We conclude that the extracellular matrix, extracellular spaces and basement membranes may delimit crest migration in the mouse.

scholarly journals Soluble and cell-bound forms of steel factor activity play distinct roles in melanocyte precursor dispersal and survival on the lateral neural crest migration pathway

Development ◽  
1995 ◽  
Vol 121 (3) ◽  
pp. 731-742 ◽  
Author(s):  
B. Wehrle-Haller ◽  
J.A. Weston
Keyword(s):  
Neural Crest ◽  
Neural Crest Cell ◽  
Soluble Form ◽  
Steel Factor ◽  
Migration Pathway ◽  
Staging Area ◽  
Trunk Neural Crest ◽  
Membrane Bound ◽  
Neural Crest Migration

Trunk neural crest cells segregate from the neuroepithelium and enter a ‘migration staging area’ lateral to the embryonic neural tube. After some crest cells in the migration staging area have begun to migrate on a medial pathway, a subpopulation of crest-derived cells remaining in the migration staging area expresses mRNAs for the receptor tyrosine kinase, c-kit, and tyrosinase-related protein-2, both of which are characteristic of melanocyte precursors. These putative melanocyte precursors are subsequently observed on the lateral crest migration pathway between the dermatome and overlying epithelium, and then dispersed in nascent dermal mesenchyme. Melanocyte precursors transiently require the c-kit ligand, Steel factor for survival. Although Steel factor mRNA is transiently expressed in the dorsal dermatome before the onset of trunk neural crest cell dispersal on the lateral pathway, it is no longer produced by dermatomal cells when melanocyte precursors have dispersed in the dermal mesenchyme. To assess the role of Steel factor in migration of melanocyte precursors on the lateral pathway, we analyzed melanocyte precursor dispersal and fate on the lateral pathway of two different Sl mutants, Sl, a null allele, and Sld, which lacks cell surface-associated Steel factor but produces a soluble form. No melanocyte precursors were detected in the dermatome of embryos homozygous for the Sl allele or in W mutants that lack functional c-kit. In contrast, in embryos homozygous for the Sld allele, melanocyte precursors appeared on the lateral pathway, but subsequently disappear from the dermis. These results suggest that soluble Steel factor is required for melanocyte precursor dispersal on the lateral pathway, or for their initial survival in the migration staging area. In contrast, membrane-bound Steel factor appears to promote melanocyte precursor survival in the dermis.

scholarly journals In Vivo Quantitative Imaging Provides Insights into Trunk Neural Crest Migration

Cell Reports ◽  
2019 ◽  
Vol 26 (6) ◽  
pp. 1489-1500.e3 ◽  
Author(s):  
Yuwei Li ◽  
Felipe M. Vieceli ◽  
Walter G. Gonzalez ◽  
Ang Li ◽  
Weiyi Tang ◽  
...  
Keyword(s):  
Neural Crest ◽  
Quantitative Imaging ◽  
Trunk Neural Crest ◽  
Neural Crest Migration

scholarly journals Interactions of Eph-related receptors and ligands confer rostrocaudal pattern to trunk neural crest migration

1997 ◽  
Vol 7 (8) ◽  
pp. 571-580 ◽  
Author(s):  
Catherine E. Krull ◽  
Rusty Lansford ◽  
Nicholas W. Gale ◽  
Andres Collazo ◽  
Christophe Marcelle ◽  
...  
Keyword(s):  
Neural Crest ◽  
Trunk Neural Crest ◽  
Neural Crest Migration

scholarly journals Segmental migration of trunk neural crest: time-lapse analysis reveals a role for PNA-binding molecules

Development ◽  
1995 ◽  
Vol 121 (11) ◽  
pp. 3733-3743 ◽  
Author(s):  
C.E. Krull ◽  
A. Collazo ◽  
S.E. Fraser ◽  
M. Bronner-Fraser
Keyword(s):  
Neural Crest ◽  
Neural Crest Cells ◽  
Time Lapse ◽  
Light Level ◽  
Explant Culture ◽  
Peanut Agglutinin ◽  
Trunk Neural Crest ◽  
Neural Crest Migration ◽  
Molecular Bases ◽  
First Time

Trunk neural crest cells migrate through the somites in a striking segmental fashion, entering the rostral but not caudal sclerotome, via cues intrinsic to the somites. Attempts to define the molecular bases of these cues have been hampered by the lack of an accessible assay system. To examine trunk neural crest migration over time and to perturb candidate guiding molecules, we have developed a novel explant preparation. Here, we demonstrate that trunk regions of the chicken embryo, placed in explant culture, continue to develop apparently normally for 2 days. Neural crest cells, recognized by prelabeling with DiI or by poststaining with the HNK-1 antibody, migrate in the somites of the explants in their typical segmental pattern. Furthermore, this paradigm allows us to follow trunk neural crest migration in situ for the first time using low-light-level videomicroscopy. The trajectories of individual neural crest cells were often complex, with cells migrating in an episodic mode encompassing forward, backward and lateral movements. Frequently, neural crest cells migrated in close-knit groups of 2–4 cells, moving at mean rates of migration of 10–14 microns/hour. Treatment of the explants with the lectin peanut agglutinin (PNA) both slowed the rate and altered the pattern of neural crest migration. Neural crest cells entered both the rostral and caudal halves of the sclerotome with mean rates of migration ranging from 6 to 13 microns/hour. These results suggest that peanut agglutinin-binding molecules are required for the segmental patterning of trunk neural crest migration. Because this approach permits neural crest migration to be both observed and perturbed, it offers the promise of more direct assays of the factors that influence neural crest development.

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