scholarly journals DNA Microsatellite Markers forSwartzia glazioviana(Fabaceae), a Threatened Species from the Brazilian Atlantic Forest

2016 ◽  
Vol 4 (2) ◽  
pp. 1500081 ◽  
Author(s):  
Janaína Spoladore ◽  
Vidal F. Mansano ◽  
Luan C. Dias de Freitas ◽  
Alexandre M. Sebbenn ◽  
Maristerra R. Lemes
PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2416 ◽  
Author(s):  
Catherine Jan ◽  
Luca Fumagalli

The parrot family represents one of the bird group with the largest number of endangered species, as a result of habitat destruction and illegal trade. This illicit traffic involves the smuggling of eggs and animals, and the laundering through captive breeding facilities of wild-caught animals. Despite the huge potential of wildlife DNA forensics to determine with conclusive evidence illegal trade, current usage of DNA profiling approaches in parrots has been limited by the lack of suitable molecular markers specifically developed for the focal species and by low cross-species polymorphism. In this study, we isolated DNA microsatellite markers in seven parrot species threatened with extinction (Amazona brasiliensis,A. oratrix,A. pretrei,A. rhodocorytha,Anodorhynchus leari,Ara rubrogenysandPrimolius couloni). From an enriched genomic library followed by 454 pyrosequencing, we characterized a total of 106 polymorphic microsatellite markers (mostly tetranucleotides) in the seven species and tested them across an average number of 19 individuals per species. The mean number of alleles per species and across loci varied from 6.4 to 8.3, with the mean observed heterozygosities ranging from 0.65 to 0.84. Identity and parentage exclusion probabilities were highly discriminatory. The high variability displayed by these microsatellite loci demonstrates their potential utility to perform individual genotyping and parentage analyses, in order to develop a DNA testing framework to determine illegal traffic in these threatened species.


2014 ◽  
Vol 13 (3) ◽  
pp. 6920-6925 ◽  
Author(s):  
K.D. Schwarcz ◽  
M.M. Bajay ◽  
C.M.T. Macrini ◽  
V.L.P. Salazar ◽  
A.P. Souza ◽  
...  

2017 ◽  
Vol 57 (27) ◽  
pp. 347 ◽  
Author(s):  
Igor Joventino Roberto ◽  
Cícero Ricardo de Oliveira ◽  
João Antonio de Araújo Filho ◽  
Herivelto Faustino de Oliveira ◽  
Robson Waldemar Ávila

The Serra do Urubu mountain range is considered a key biodiversity area. It is situated in the Pernambuco Endemism Center, one of the most threatened regions of the Brazilian Atlantic Forest. However, despite the high importance of this area little research on its herpetofauna has been performed. The present study presents an inventory of the herpetofauna of the region, through bibliographic review, searches in museum collections and field expeditions to the RPPNs Frei Caneca and Pedra D’Antas, in the municipalities of Jaqueira and Lagoa dos Gatos. The conservation status of the amphibians of the region is discussed. Five expeditions, between 2012 and 2013 were made. The methods employed were visual transect surveys, acoustic census and pitfall traps. We recorded a total of 46 amphibian species, belonging to nine families: Craugastoridae (3 spp.), Bufonidae (3 spp.), Ranidae (1 sp.), Hylidae (25 spp.), Leptodactylidae (8 spp.), Odontophrynidae (1 sp.), Hemiphractidae (2 spp.), Phyllomedusidae (2 spp.) and Microhylidae (1 sp.). We recorded 42 species of squamates: 16 species of lizards families Phyllodactylidae (1 sp.), Gekkonidae (1 sp.), Gymnophthalmidae (1 sp.), Polychrotidae (1 sp.), Leiosauridae (1 sp.), Tropiduridae (3 spp.), Dactyloidae (2 spp.), Diploglossidae (2 spp.), Teiidae (2 spp.), Scincidae (1 sp.), and Iguanidae (1 sp.); and 24 species of snakes: Boidae (3 spp.), Colubridae (2 spp.), Dipsadidae (13 spp.), Elapidae (2 spp.), Typhlopidae (1 sp.), and Viperidae (3 spp.). The occurrence of rare and/or threatened species such as the snakes Dipsas sazimai, Lachesis muta and Sibynomorphus sp. and the amphibians Hylomantis granulosa, Chiasmocleis alagoana, Boana freicanecae and Phyllodytes gyrinaethes reinforces the need for conservation measures at this highly threatened region of the Atlantic Forest.


1997 ◽  
Vol 140 (17) ◽  
pp. 446-449 ◽  
Author(s):  
J. L. Williams ◽  
A. P. Usha ◽  
B. G. D. Urquhart ◽  
M. Kilroy

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