scholarly journals Characterization of Nuclear Microsatellite Markers forRumex bucephalophorus(Polygonaceae) Using 454 Sequencing

2015 ◽  
Vol 3 (12) ◽  
pp. 1500088 ◽  
Author(s):  
Juan Viruel ◽  
Pedro L. Ortiz ◽  
Montserrat Arista ◽  
María Talavera
2018 ◽  
Vol 65 (5) ◽  
pp. 1337-1348 ◽  
Author(s):  
Margherita Rodolfi ◽  
Tommaso Ganino ◽  
Benedetta Chiancone ◽  
Raffaella Petruccelli

2014 ◽  
Vol 2 (12) ◽  
pp. 1400093 ◽  
Author(s):  
Maria von Cräutlein ◽  
Helena Korpelainen ◽  
Marjo Helander ◽  
Annika Öhberg ◽  
Kari Saikkonen

2014 ◽  
Vol 2 (6) ◽  
pp. 1400020 ◽  
Author(s):  
Gustavo H. F. Klabunde ◽  
Denise Olkoski ◽  
Vinicius Vilperte ◽  
Maria I. Zucchi ◽  
Rubens O. Nodari

Biologia ◽  
2013 ◽  
Vol 68 (4) ◽  
Author(s):  
Konrad Celiński ◽  
Ewa Pawlaczyk ◽  
Aleksandra Wojnicka-Półtorak ◽  
Ewa Chudzińska ◽  
Wiesław Prus-Głowacki

AbstractPinus mugo (dwarf mountain pine) is an important component of European mountain ecosystems. However, little is known about the present genetic structure and population differentiation of this species at the DNA level, possibly due to a lack of nuclear microsatellite markers (SSR) developed for Pinus mugo. Therefore in this study we transferred microsatellite markers originally developed for Pinus sylvestris and Pinus taeda to Pinus mugo. This cross-species amplification approach is much faster and less expensive than isolation and characterization of new microsatellite markers. The transfer rates from the source species to Pinus mugo were moderately low (26%). There were no differences in microsatellite repeat motifs between the source species and Pinus mugo. Nuclear microsatellite markers successfully transferred to Pinus mugo can be applied to various genetic studies on this species, due to the high level of their polymorphism and high value of polymorphic information content.


2014 ◽  
Vol 63 (1-6) ◽  
pp. 109-112 ◽  
Author(s):  
Dan-Dan Zhang ◽  
Pi Luo ◽  
Ying Chen ◽  
Zheng-Feng Wang ◽  
Wan-Hui Ye ◽  
...  

Abstract Engelhardia roxburghiana is a common half evergreen tree with a wide distribution in southeast Asia. Despite its ecological and pharmaceutical values, its genetic diversity is poorly studied. Our objective was to develop nuclear microsatellite markers to investigate the level of genetic diversity within and among populations in the future. Using the microsatellite-enriched library and PCR-based screening method, 12 microsatellite markers were developed and showed polymorphism in a population. The number of alleles per locus for these 12 microsatellites ranged from four to 15. The observed and expected heterozygosities ranged from 0.358 to 0.897 and from 0.369 to 0.886, respectively. The developed microsatellites will be useful for studying genetic diversity and population structure in E. roxburghiana.


OENO One ◽  
2005 ◽  
Vol 39 (3) ◽  
pp. 109
Author(s):  
Emmanuel D. Ladoukakis ◽  
François Lefort ◽  
Petraq Sotiri ◽  
Arjola Bacu ◽  
Efigjeni Kongjika ◽  
...  

<p style="text-align: justify;">A recently restored ampelographic collection of Albanian grapevine accessions has been submitted to genetic profiling with eleven nuclear microsatellite markers, widely used in other studies. Microsatellite profiling resulted in 28 single profiles for 29 accessions. Two cultivars, Shesh I bardhë and Pucalla, were found to be synonyms. Genetic profiles of Albanian cultivars were compared at 8 microsatellite loci to 29 most commonly cultivated Greek cultivars. Albanian cultivars were found to be more closely related to Greek cultivars from Peloponnese. One Greek cultivar named Dempina was found to be genetically close to two Albanian cultivars Debina teki and Debina kala, which are homonyms Another cultivar, known as Toska or Sinambel displayed a tri-allelic profile at 5 loci over 10 analysed loci. Such a high number of tri-allelic loci found in one individual favours the hypothesis of triploidy but the chimerism hypothesis cannot be excluded without further work.</p>


2020 ◽  
Vol 25 (2) ◽  
pp. 120-123
Author(s):  
Taishi Sakagami ◽  
Shota Sakaguchi ◽  
Yuji Isagi ◽  
Hiroaki Setoguchi

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