Development and Characterization of Nuclear Microsatellite Markers in the Endophytic FungusEpichloë festucae(Clavicipitaceae)

AbstractPinus mugo (dwarf mountain pine) is an important component of European mountain ecosystems. However, little is known about the present genetic structure and population differentiation of this species at the DNA level, possibly due to a lack of nuclear microsatellite markers (SSR) developed for Pinus mugo. Therefore in this study we transferred microsatellite markers originally developed for Pinus sylvestris and Pinus taeda to Pinus mugo. This cross-species amplification approach is much faster and less expensive than isolation and characterization of new microsatellite markers. The transfer rates from the source species to Pinus mugo were moderately low (26%). There were no differences in microsatellite repeat motifs between the source species and Pinus mugo. Nuclear microsatellite markers successfully transferred to Pinus mugo can be applied to various genetic studies on this species, due to the high level of their polymorphism and high value of polymorphic information content.

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Short Note: Isolation and Characterization of 12 Polymorphic Microsatellite Markers in Engelhardia roxburghiana (Juglandaceae)

Silvae Genetica ◽

10.1515/sg-2014-0014 ◽

2014 ◽

Vol 63 (1-6) ◽

pp. 109-112 ◽

Cited By ~ 2

Author(s):

Dan-Dan Zhang ◽

Pi Luo ◽

Ying Chen ◽

Zheng-Feng Wang ◽

Wan-Hui Ye ◽

...

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Short Note ◽

Screening Method ◽

Wide Distribution ◽

Enriched Library ◽

Isolation And Characterization ◽

Nuclear Microsatellite ◽

Polymorphic Microsatellite

Abstract Engelhardia roxburghiana is a common half evergreen tree with a wide distribution in southeast Asia. Despite its ecological and pharmaceutical values, its genetic diversity is poorly studied. Our objective was to develop nuclear microsatellite markers to investigate the level of genetic diversity within and among populations in the future. Using the microsatellite-enriched library and PCR-based screening method, 12 microsatellite markers were developed and showed polymorphism in a population. The number of alleles per locus for these 12 microsatellites ranged from four to 15. The observed and expected heterozygosities ranged from 0.358 to 0.897 and from 0.369 to 0.886, respectively. The developed microsatellites will be useful for studying genetic diversity and population structure in E. roxburghiana.

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Genetic characterization of Albanian grapevine cultivars by microsatellite markers

OENO One ◽

10.20870/oeno-one.2005.39.3.895 ◽

2005 ◽

Vol 39 (3) ◽

pp. 109

Author(s):

Emmanuel D. Ladoukakis ◽

François Lefort ◽

Petraq Sotiri ◽

Arjola Bacu ◽

Efigjeni Kongjika ◽

...

Keyword(s):

Microsatellite Markers ◽

Microsatellite Loci ◽

Genetic Characterization ◽

Allelic Profile ◽

Genetic Profiling ◽

Nuclear Microsatellite ◽

Genetic Profiles

<p style="text-align: justify;">A recently restored ampelographic collection of Albanian grapevine accessions has been submitted to genetic profiling with eleven nuclear microsatellite markers, widely used in other studies. Microsatellite profiling resulted in 28 single profiles for 29 accessions. Two cultivars, Shesh I bardhë and Pucalla, were found to be synonyms. Genetic profiles of Albanian cultivars were compared at 8 microsatellite loci to 29 most commonly cultivated Greek cultivars. Albanian cultivars were found to be more closely related to Greek cultivars from Peloponnese. One Greek cultivar named Dempina was found to be genetically close to two Albanian cultivars Debina teki and Debina kala, which are homonyms Another cultivar, known as Toska or Sinambel displayed a tri-allelic profile at 5 loci over 10 analysed loci. Such a high number of tri-allelic loci found in one individual favours the hypothesis of triploidy but the chimerism hypothesis cannot be excluded without further work.</p>

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Development and characterization of nuclear microsatellite markers in Aphananthe aspera (Thunb.) Planch. (Cannabaceae)

Journal of Forest Research ◽

10.1080/13416979.2020.1744232 ◽

2020 ◽

Vol 25 (2) ◽

pp. 120-123

Author(s):

Taishi Sakagami ◽

Shota Sakaguchi ◽

Yuji Isagi ◽

Hiroaki Setoguchi

Keyword(s):

Microsatellite Markers ◽

Nuclear Microsatellite ◽

Aphananthe Aspera

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Isolation and characterization of 15 nuclear microsatellite markers forBaillonella toxisperma Pierre(Sapotaceae), a low-density tree species of Central Africa

Molecular Ecology Resources ◽

10.1111/j.1755-0998.2009.02543.x ◽

2009 ◽

Vol 9 (4) ◽

pp. 1135-1138 ◽

Cited By ~ 5

Author(s):

D. NDIADE-BOUROBOU ◽

A. VAILLANT ◽

B. FAVREAU ◽

E. GAYRIN ◽

J. M. BOUVET

Keyword(s):

Microsatellite Markers ◽

Tree Species ◽

Central Africa ◽

Low Density ◽

Isolation And Characterization ◽

Nuclear Microsatellite

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Characterization of Nuclear Microsatellite Markers for the Narrow Endemic Syringa josikaea Jacq. fil. ex Rchb.

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha4119023 ◽

2013 ◽

Vol 41 (1) ◽

pp. 301 ◽

Cited By ~ 2

Author(s):

Bertalan LENDVAY ◽

Andrzej PEDRYC ◽

Mária Höhn

Keyword(s):

Microsatellite Markers ◽

Ornamental Plants ◽

Enriched Library ◽

Microsatellite Primers ◽

Genetic Lineages ◽

Nuclear Microsatellite ◽

Rare Endemic ◽

Two Populations ◽

Pcr Conditions

The species of the genus Syringa L. are among the most popular ornamental plants worldwide. One particular species, Syringa josikaea, a rare endemic of the Carpathian Mountains, is of great conservation interest. Although microsatellite markers may be useful for studying the genetic variability of varieties and populations, no microsatellites have previously been characterized for any species of the Syringa genus. Our aim was therefore to test the applicability of microsatellite primers developed for neighboring genera (Olea and Ligustrum) and to complement these with markers isolated and characterized for S. josikaea. Twelve primer pairs of Olea and Ligustrum were tested by optimizing PCR conditions and checking the variability in 40 samples of two populations of S. josikaea. Two of them proved to be easy to PCR amplify and variable at the same time. To develop new primers we constructed a microsatellite enriched library and sequenced 48 clones. 18 sequences contained microsatellite motifs, and three of the designed primer pairs presented high allele variability. The five primer pairs characterized for S. josikaea proved to be highly informative and sufficient to distinguish between individuals. These microsatellite primers are valuable tools to study genetic variation of native populations, genetic lineages of hybrids and cultivars of S. josikaea.

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