scholarly journals In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers

10.3791/51679 ◽  
2014 ◽  
Author(s):  
Chandirasegaran Massilamany ◽  
Arunakumar Gangaplara ◽  
Ting Jia ◽  
Christian Elowsky ◽  
Qingsheng Li ◽  
...  
Keyword(s):  
T Cells ◽  
Major Histocompatibility Complex ◽  
Major Histocompatibility Complex Class ◽  
Cd4 T Cells ◽  
Class Ii ◽  
Complex Class ◽  
Major Histocompatibility ◽  
Histocompatibility Complex

scholarly journals Direct Staining with Major Histocompatibility Complex Class II Dextramers Permits Detection of Antigen-Specific, Autoreactive CD4 T Cells In Situ

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e87519 ◽  
Author(s):  
Chandirasegaran Massilamany ◽  
Arunakumar Gangaplara ◽  
Ting Jia ◽  
Christian Elowsky ◽  
Guobin Kang ◽  
...  
Keyword(s):  
T Cells ◽  
Major Histocompatibility Complex ◽  
Major Histocompatibility Complex Class ◽  
Cd4 T Cells ◽  
Class Ii ◽  
Complex Class ◽  
Major Histocompatibility ◽  
Histocompatibility Complex

scholarly journals Detection of autoreactive CD4 T cells using major histocompatibility complex class II dextramers

2011 ◽  
Vol 12 (1) ◽  
pp. 40 ◽  
Author(s):  
Chandirasegaran Massilamany ◽  
Bijaya Upadhyaya ◽  
Arunakumar Gangaplara ◽  
Charles Kuszynski ◽  
Jay Reddy
Keyword(s):  
T Cells ◽  
Major Histocompatibility Complex ◽  
Major Histocompatibility Complex Class ◽  
Cd4 T Cells ◽  
Class Ii ◽  
Complex Class ◽  
Major Histocompatibility ◽  
Histocompatibility Complex

scholarly journals Mycobacterium tuberculosis Promotes HIV trans-Infection and Suppresses Major Histocompatibility Complex Class II Antigen Processing by Dendritic Cells

2010 ◽  
Vol 84 (17) ◽  
pp. 8549-8560 ◽  
Author(s):  
Morgan A. Reuter ◽  
Nicole D. Pecora ◽  
Clifford V. Harding ◽  
David H. Canaday ◽  
David McDonald
Keyword(s):  
T Cells ◽  
Major Histocompatibility Complex ◽  
Major Histocompatibility Complex Class ◽  
Cd4 T Cells ◽  
Antigen Processing ◽  
Class Ii ◽  
Sub Saharan Africa ◽  
Complex Class ◽  
Major Histocompatibility ◽  
Histocompatibility Complex

ABSTRACT Mycobacterium tuberculosis is a leading killer of HIV-infected individuals worldwide, particularly in sub-Saharan Africa, where it is responsible for up to 50% of HIV-related deaths. Infection by HIV predisposes individuals to M. tuberculosis infection, and coinfection accelerates the progression of both diseases. In contrast to most other opportunistic infections associated with HIV, an increased risk of M. tuberculosis infection occurs during early-stage HIV disease, long before CD4 T cell counts fall below critical levels. We hypothesized that M. tuberculosis infection contributes to HIV pathogenesis by interfering with dendritic cell (DC)-mediated immune control. DCs carry pathogens like M. tuberculosis and HIV from sites of infection into lymphoid tissues, where they process and present antigenic peptides to CD4 T cells. Paradoxically, DCs can also deliver infectious HIV to T cells without first becoming infected, a process known as trans-infection. Lipopolysaccharide (LPS)-activated DCs sequester HIV in pocketlike membrane invaginations that remain open to the cell surface, and individual virions are delivered from the pocket into T cells at the site of contact during trans-infection. Here we report that M. tuberculosis exposure increases HIV trans-infection and induces viral sequestration within surface-accessible compartments identical to those seen in LPS-stimulated DCs. At the same time, M. tuberculosis dramatically decreases the degradative processing and major histocompatibility complex class II (MHC-II) presentation of HIV antigens to CD4 T cells. Our data suggest that M. tuberculosis infection promotes a shift in the dynamic balance between antigen processing and intact virion presentation, favoring DC-mediated amplification of HIV infections.

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