Facial Nerve Surgery in the Rat Model to Study Axonal Inhibition and Regeneration

10.3791/59224 ◽  
2020 ◽  
Author(s):  
Syed Ahmed Ali ◽  
Aaron W. Stebbins ◽  
John E. Hanks ◽  
Robbi A. Kupfer ◽  
Norman D. Hogikyan ◽  
...  
Keyword(s):  
Facial Nerve ◽  
Rat Model ◽  
Facial Nerve Surgery ◽  
Nerve Surgery

Recent advances in facial nerve surgery

1982 ◽  
Vol 4 (3) ◽  
pp. 233-236
Author(s):  
Roger L. Crumley
Keyword(s):  
Facial Nerve ◽  
Recent Advances ◽  
Facial Nerve Surgery ◽  
Nerve Surgery

Facial Nerve Surgery

1989 ◽  
pp. 97-103
Author(s):  
Vittorio Colletti ◽  
James E. Benecke
Keyword(s):  
Facial Nerve ◽  
Facial Nerve Surgery ◽  
Nerve Surgery

Facial Nerve Surgery

2015 ◽  
pp. 159-159
Author(s):  
Eric Sargent ◽  
Jack Kartush
Keyword(s):  
Facial Nerve ◽  
Facial Nerve Surgery ◽  
Nerve Surgery

Facial Nerve Surgery

1977 ◽  
Vol 103 (12) ◽  
pp. 747-747
Author(s):  
B. R. ALFORD
Keyword(s):  
Facial Nerve ◽  
Facial Nerve Surgery ◽  
Nerve Surgery

Study on facial nerve activation pathway based on nanometric magnetic beads

2020 ◽  
Vol 10 (11) ◽  
pp. 1808-1815
Author(s):  
Yang Yuan ◽  
Liheng Li ◽  
Wenwen Liu ◽  
Hong Liu ◽  
Qijun Fan ◽  
...  
Keyword(s):  
Facial Nerve ◽  
Western Blotting ◽  
Magnetic Beads ◽  
Morphological Changes ◽  
Control Group ◽  
Rt Pcr ◽  
Gene Target ◽  
Facial Nerve Surgery ◽  
Operation Group ◽  
Nerve Surgery

This study was conducted to investigate the mechanism of facial nerve recovery through Toll-like receptor 2 (TLR2)/nuclear factor kappa B (NF-κB) after facial nerve trunk damage. Facial nerve surgery was performed on rats to establish a paralyzed animal model with or without intraperitoneal (i.p.) injection of Pam3CSK4 (TLR2 agonist). All nucleic acids were extracted using nanometer magnetic beads. At 1, 4, 7, 10, 13, and 16 days after the surgery, the score of the blink reflex, the motion of vibrissae, and the motion of nose were analyzed to assess the function of facial nerves. Evaluated using transmission electron microscopy (TEM) and immunofluorescence staining (IF), Morphological changes in facial nerve and brainstem were analyzed. The expressions of TLR2 and NF-κB p65 in the brainstem were detected by quantitative Reverse Transcription-Polymerase Chain Reaction (RT-PCR), western blotting, and IF. TEM revealed that the facial nerve fiber diameter was shortened, and the organelle was swollen and demyelinated in the operation group. The IF results revealed that the expressions of NF-κB p65 and TLR2 in the brainstem were increased significantly in the operation group together with the control group. RT-PCR demonstrated that the mRNA expressions of TLR2 and NF-κB p65 in the brainstem were low in the control group, as well as in the sham group, whereas there were significant increases in the mRNA expressions of TLR2 and NF-κB p65 in the operation group. Western blotting revealed a significant increase in the expression of TLR2 protein after the surgery compared to that in the control group. After subjecting the rats to facial nerve surgery and i.p. injection of Pam3CSK4, the protein expressions of TLR2 and NF-κB p65 were upregulated dramatically in the Pam3CSK4-operation group compared to that in the control group and were also significantly increased than those in the operation group. Both facial nerve dysfunction caused due to facial nerve surgery (exacerbated by co-treatment with Pam3CSK4) and the expressions of NF-κB p65 and TLR2 were increased significantly. TLR2 might be an innovative gene target and a novel option for the clinical applications of facial paralysis immunotherapy.

History of Facial Nerve Surgery

2002 ◽  
Vol 23 (Sup 1) ◽  
pp. S1 ◽  
Author(s):  
Mark May ◽  
Barry M. Schaitkin
Keyword(s):  
Facial Nerve ◽  
Facial Nerve Surgery ◽  
History Of ◽  
Nerve Surgery

Extratemporal Facial Nerve Surgery

1972 ◽  
Vol 95 (4) ◽  
pp. 369-375 ◽  
Author(s):  
T. Kitamura ◽  
K. Togawa ◽  
K. Tsukamoto ◽  
J. Naito
Keyword(s):  
Facial Nerve ◽  
Facial Nerve Surgery ◽  
Nerve Surgery

New Aspects in Facial Nerve Surgery

1979 ◽  
Vol 6 (3) ◽  
pp. 451-470 ◽  
Author(s):  
Adolf Miehlke ◽  
Eberhard Stennert ◽  
Reinhard Chilla
Keyword(s):  
Facial Nerve ◽  
Facial Nerve Surgery ◽  
Nerve Surgery
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