scholarly journals Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

10.3791/62008 ◽  
2020 ◽  
Author(s):  
Kelly Veerasammy ◽  
Yuki X. Chen ◽  
Sami Sauma ◽  
Mathilde Pruvost ◽  
David K. Dansu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Metabolic Profiling ◽  
Mass Spectrometry Imaging ◽  
Maldi Mass Spectrometry ◽  
Maldi Mass Spectrometry Imaging

scholarly journals Sample preparation strategy for the detection of steroid-like compounds using MALDI mass spectrometry imaging: pulmonary distribution of budesonide as a case study

2021 ◽  
Author(s):  
Riccardo Zecchi ◽  
Pietro Franceschi ◽  
Laura Tigli ◽  
Davide Amidani ◽  
Chiara Catozzi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Mass Spectrometry Imaging ◽  
Maldi Mass Spectrometry ◽  
Adult Rabbit ◽  
Chronic Obstructive ◽  
Ionization Mass ◽  
Obstructive Pulmonary Disease ◽  
Imaging Protocol ◽  
Girard’S Reagent

AbstractCorticosteroids as budesonide can be effective in reducing topic inflammation processes in different organs. Therapeutic use of budesonide in respiratory diseases, like asthma, chronic obstructive pulmonary disease, and allergic rhinitis is well known. However, the pulmonary distribution of budesonide is not well understood, mainly due to the difficulties in tracing the molecule in lung samples without the addition of a label. In this paper, we present a matrix-assisted laser desorption/ionization mass spectrometry imaging protocol that can be used to visualize the pulmonary distribution of budesonide administered to a surfactant-depleted adult rabbit. Considering that budesonide is not easily ionized by MALDI, we developed an on-tissue derivatization method with Girard’s reagent P followed by ferulic acid deposition as MALDI matrix. Interestingly, this sample preparation protocol results as a very effective strategy to raise the sensitivity towards not only budesonide but also other corticosteroids, allowing us to track its distribution and quantify the drug inside lung samples. Graphical abstract

Matrix Sublimation Device for MALDI Mass Spectrometry Imaging

2018 ◽  
Vol 91 (1) ◽  
pp. 803-807 ◽  
Author(s):  
Roberto Fernández ◽  
Jone Garate ◽  
Lucia Martín-Saiz ◽  
Igor Galetich ◽  
José A. Fernández
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Maldi Mass Spectrometry ◽  
Maldi Mass Spectrometry Imaging
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