scholarly journals Sample preparation strategy for the detection of steroid-like compounds using MALDI mass spectrometry imaging: pulmonary distribution of budesonide as a case study

2021 ◽  
Author(s):  
Riccardo Zecchi ◽  
Pietro Franceschi ◽  
Laura Tigli ◽  
Davide Amidani ◽  
Chiara Catozzi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Mass Spectrometry Imaging ◽  
Maldi Mass Spectrometry ◽  
Adult Rabbit ◽  
Chronic Obstructive ◽  
Ionization Mass ◽  
Obstructive Pulmonary Disease ◽  
Imaging Protocol ◽  
Girard’S Reagent

AbstractCorticosteroids as budesonide can be effective in reducing topic inflammation processes in different organs. Therapeutic use of budesonide in respiratory diseases, like asthma, chronic obstructive pulmonary disease, and allergic rhinitis is well known. However, the pulmonary distribution of budesonide is not well understood, mainly due to the difficulties in tracing the molecule in lung samples without the addition of a label. In this paper, we present a matrix-assisted laser desorption/ionization mass spectrometry imaging protocol that can be used to visualize the pulmonary distribution of budesonide administered to a surfactant-depleted adult rabbit. Considering that budesonide is not easily ionized by MALDI, we developed an on-tissue derivatization method with Girard’s reagent P followed by ferulic acid deposition as MALDI matrix. Interestingly, this sample preparation protocol results as a very effective strategy to raise the sensitivity towards not only budesonide but also other corticosteroids, allowing us to track its distribution and quantify the drug inside lung samples. Graphical abstract

scholarly journals Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

10.3791/62008 ◽  
2020 ◽  
Author(s):  
Kelly Veerasammy ◽  
Yuki X. Chen ◽  
Sami Sauma ◽  
Mathilde Pruvost ◽  
David K. Dansu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Metabolic Profiling ◽  
Mass Spectrometry Imaging ◽  
Maldi Mass Spectrometry ◽  
Maldi Mass Spectrometry Imaging

scholarly journals Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging

2021 ◽  
Vol 22 (22) ◽  
pp. 12393
Author(s):  
Elvira Sgobba ◽  
Yohann Daguerre ◽  
Marco Giampà
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Maldi Mass Spectrometry ◽  
Cellular Level ◽  
Ionization Mass ◽  
Medical Sciences ◽  
Research Areas ◽  
Local Complexity ◽  
Microbe Interactions ◽  
Fine Structures

Classic metabolomic methods have proven to be very useful to study functional biology and variation in the chemical composition of different tissues. However, they do not provide any information in terms of spatial localization within fine structures. Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) does and reaches at best a spatial resolution of 0.25 μm depending on the laser setup, making it a very powerful tool to analyze the local complexity of biological samples at the cellular level. Here, we intend to give an overview of the diversity of the molecules and localizations analyzed using this method as well as to update on the latest adaptations made to circumvent the complexity of samples. MALDI MSI has been widely used in medical sciences and is now developing in research areas as diverse as entomology, microbiology, plant biology, and plant–microbe interactions, the rhizobia symbiosis being the most exhaustively described so far. Those are the fields of interest on which we will focus to demonstrate MALDI MSI strengths in characterizing the spatial distributions of metabolites, lipids, and peptides in relation to biological questions.

scholarly journals Single-Photon-Induced Post-Ionization to Boost Ion Yields in MALDI Mass Spectrometry Imaging

2021 ◽  
Author(s):  
Christoph Bookmeyer ◽  
Ulrich Röhling ◽  
Klaus Dreisewerd ◽  
Jens Soltwisch
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Single Photon ◽  
Cost Effective ◽  
Maldi Mass Spectrometry ◽  
Ion Source ◽  
Ionization Mass ◽  
Gas Phase Reactions ◽  
Ion Yields ◽  
Ion Funnel

Abstract Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a rapidly growing method in many fields of the life sciences. For many analyte classes, however, its sensitivity is limited due to poor ionization efficiencies. To mitigate this problem, we here introduce a novel and cost-effective postionization scheme at high repetition rates based on the interplay of single-photon photoionization and subsequent charge transfer reactions. Importantly, the fine vacuum conditions of a dual ion-funnel ion source effectively thermalize the evolving MALDI plume and enable ample gas-phase reactions as well as the addition of chemical dopants that crucially support chemical ionization. Supported by acetone dopant, [M + H]+/[M-H]− signals of numerous glycerophospho-, sphingo-, and further lipids, registered from mammal brain and kidney sections, were boosted by up to three orders of magnitude, similar to results obtained with laser-based postionization (MALDI-2). Experiments utilizing deuterated matrix and dopant, however, indicate complex ionization pathways different from MALDI2.

Sign in / Sign up

Export Citation Format

Share Document