scholarly journals OnePot PURE Cell-Free System

10.3791/62625 ◽  
2021 ◽  
Author(s):  
Laura Grasemann ◽  
Barbora Lavickova ◽  
M. Carolina Elizondo-Cantú ◽  
Sebastian J. Maerkl
Keyword(s):  
Free System ◽  
Cell Free System ◽  
Pure Cell

scholarly journals Buffer preparation for OnePot PURE cell-free system v1 (protocols.io.728hqhw)

protocols.io ◽  
2019 ◽  
Author(s):  
Konstantinos Ragios
Keyword(s):  
Free System ◽  
Cell Free System ◽  
Pure Cell

scholarly journals A simple, robust, and low-cost method to produce the PURE cell - free system

2018 ◽  
Author(s):  
Barbora Lavickova ◽  
Sebastian J. Maerkl
Keyword(s):  
Protein Synthesis ◽  
Low Cost ◽  
Translation System ◽  
Free System ◽  
Cell Free System ◽  
Pure System ◽  
Pure Cell ◽  
Synthesis Yield

AbstractWe demonstrate a simple, robust, and low-cost method for producing the PURE cell-free transcription-translation system. Our OnePot PURE system achieved a protein synthesis yield of 156 µg/mL at a cost of 0.09 USD/µL, leading to a 14-fold improvement in cost normalized protein synthesis yield over existing PURE systems. The OnePot method makes the PURE system easy to generate and allows it to be readily optimized and modified.

scholarly journals Ribosome Purification for OnePot PURE cell-free system v1 (protocols.io.5bbg2in)

protocols.io ◽  
2019 ◽  
Author(s):  
Konstantinos Ragios
Keyword(s):  
Free System ◽  
Cell Free System ◽  
Pure Cell

scholarly journals A Simple, Robust, and Low-Cost Method To Produce the PURE Cell-Free System

2019 ◽  
Vol 8 (2) ◽  
pp. 455-462 ◽  
Author(s):  
Barbora Lavickova ◽  
Sebastian J. Maerkl
Keyword(s):  
Low Cost ◽  
Free System ◽  
Cell Free System ◽  
Pure Cell
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