Efficient and Cost Effective Electroporation Method to Study Primary Cilium-dependent Signaling Pathways in the Granule Cell Precursor

10.3791/63283 ◽  
2021 ◽  
Author(s):  
Jason Cho Wai Lo ◽  
Wee Lin Wong ◽  
Catherine Hong Huan Hor
Keyword(s):  
Signaling Pathways ◽  
Primary Cilium ◽  
Granule Cell ◽  
Cost Effective ◽  
Cell Precursor ◽  
Granule Cell Precursor

scholarly journals Multifaceted functions of Rab23 on primary cilium and Hedgehog signaling-mediated granule cell proliferation

2020 ◽  
Author(s):  
CHH Hor ◽  
WY Leong ◽  
ELK Goh
Keyword(s):  
Sonic Hedgehog ◽  
Primary Cilium ◽  
Granule Cell ◽  
Hedgehog Signaling ◽  
Cell Precursor ◽  
Shh Signaling ◽  
Shh Pathway ◽  
Granule Cell Precursor

AbstractSonic Hedgehog (Shh) signaling from the primary cilium drives cerebellar granule cell precursor (GCP) proliferation. Mutations of hedgehog (Hh) pathway repressors could cause medulloblastoma, the most prevalent and malignant childhood brain tumor that arises from aberrant GCP proliferation. We demonstrate that brain-specific knockout of a Shh pathway repressor Rab23 in mice caused mis-patterning of cerebellar folia and elevated GCP proliferation during early development, but with no prevalent occurrence of medulloblastoma at adult stage. Strikingly, Rab23-depleted GCPs exhibited up-regulated basal level of Shh pathway activities despite reduced ciliation, and were desensitized against stimulations by Shh and Smoothened (Smo) agonist in primary GCP culture. These results illustrate dual functions of Rab23 in repressing the basal level of Shh signaling, while facilitating Shh signal transduction via Shh/Smo on primary cilium. Collectively, our findings unravel instrumental roles of Rab23 in GCP proliferation and ciliogenesis. Rab23’s potentiation of Shh signaling pathway through the primary cilium and Smo, suggests a potential new therapeutic for Smo/primary cilium-driven medulloblastoma.Author SummaryC.H.H conceived, designed, lead, and performed all in vitro and in vivo experiments, analyzed data and wrote the manuscript. W.Y performed QPCR experiments and primary GCP cultures and analyzed data. E.L.G conceived and directed the study.

scholarly journals LKB1 Regulates Cerebellar Development by Controlling Sonic Hedgehog-mediated Granule Cell Precursor Proliferation and Granule Cell Migration

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Yuqin Men ◽  
Aizhen Zhang ◽  
Haixiang Li ◽  
Yecheng Jin ◽  
Xiaoyang Sun ◽  
...  
Keyword(s):  
Cell Migration ◽  
Sonic Hedgehog ◽  
Granule Cell ◽  
Cerebellar Development ◽  
Cell Precursor ◽  
Granule Cell Precursor

scholarly journals Differential Modulation of Sonic-Hedgehog-Induced Cerebellar Granule Cell Precursor Proliferation by the IGF Signaling Network

2010 ◽  
Vol 32 (1) ◽  
pp. 59-70 ◽  
Author(s):  
Carla Fernandez ◽  
Valérie M. Tatard ◽  
Nicolas Bertrand ◽  
Nadia Dahmane
Keyword(s):  
Sonic Hedgehog ◽  
Granule Cell ◽  
Signaling Network ◽  
Cerebellar Granule Cell ◽  
Differential Modulation ◽  
Cell Precursor ◽  
Cerebellar Granule ◽  
Granule Cell Precursor ◽  
Igf Signaling

[P2.73]: Reduced granule cell precursor proliferation in the cerebellum of fetuses with Down syndrome

2010 ◽  
Vol 28 (8) ◽  
pp. 712-712
Author(s):  
S. Guidi ◽  
P. Bonasoni ◽  
E. Ciani ◽  
R. Bartesaghi
Keyword(s):  
Down Syndrome ◽  
Granule Cell ◽  
Cell Precursor ◽  
Granule Cell Precursor

scholarly journals PERK Activation Promotes Medulloblastoma Tumorigenesis by Attenuating Premalignant Granule Cell Precursor Apoptosis

2016 ◽  
Vol 186 (7) ◽  
pp. 1939-1951 ◽  
Author(s):  
Yeung Ho ◽  
Xiting Li ◽  
Stephanie Jamison ◽  
Heather P. Harding ◽  
Peter J. McKinnon ◽  
...  
Keyword(s):  
Granule Cell ◽  
Cell Precursor ◽  
Granule Cell Precursor

scholarly journals Nos2 Inactivation Promotes the Development of Medulloblastoma in Ptch1+/− Mice by Deregulation of Gap43–Dependent Granule Cell Precursor Migration

PLoS Genetics ◽  
2012 ◽  
Vol 8 (3) ◽  
pp. e1002572 ◽  
Author(s):  
Daniel Haag ◽  
Petra Zipper ◽  
Viola Westrich ◽  
Daniela Karra ◽  
Karin Pfleger ◽  
...  
Keyword(s):  
Granule Cell ◽  
Cell Precursor ◽  
Granule Cell Precursor

MATH-1 production by an adult medulloblastoma suggestive of a cerebellar external granule cell precursor origin

2008 ◽  
Vol 15 (1) ◽  
pp. 84-87 ◽  
Author(s):  
Tetsuya Ueba ◽  
Eiji Kadota ◽  
Hideyuki Kano ◽  
Kohsuke Yamashita ◽  
Naoki Kageyama
Keyword(s):  
Granule Cell ◽  
Cell Precursor ◽  
Adult Medulloblastoma ◽  
Granule Cell Precursor

scholarly journals A Role for p27/Kip1 in the Control of Cerebellar Granule Cell Precursor Proliferation

2000 ◽  
Vol 20 (15) ◽  
pp. 5756-5763 ◽  
Author(s):  
Kazuhiro Miyazawa ◽  
Toshiyuki Himi ◽  
Veronica Garcia ◽  
Hisakazu Yamagishi ◽  
Shigeaki Sato ◽  
...  
Keyword(s):  
Granule Cell ◽  
Cerebellar Granule Cell ◽  
Cell Precursor ◽  
Cerebellar Granule ◽  
Granule Cell Precursor ◽  
P27 Kip1
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