Molecular cloning and characterization of human WINS1 and mouse Wins2, homologous to Drosophila segment polarity gene Lines (Lin)

2002 ◽  
Author(s):  
Masaru Katoh
Keyword(s):  
Molecular Cloning ◽  
Segment Polarity ◽  
Segment Polarity Gene ◽  
Polarity Gene

Molecular cloning and initial characterization of the MG61/PORC gene, the human homologue of the Drosophila segment polarity gene Porcupine

Gene ◽  
2002 ◽  
Vol 288 (1-2) ◽  
pp. 147-157 ◽  
Author(s):  
Andrea Caricasole ◽  
Teresa Ferraro ◽  
Joseph M. Rimland ◽  
Georg C. Terstappen
Keyword(s):  
Molecular Cloning ◽  
Human Homologue ◽  
Initial Characterization ◽  
Segment Polarity ◽  
Segment Polarity Gene ◽  
Polarity Gene

Isolation and Characterization of a Mouse Homolog of the Drosophila Segment Polarity Gene dishevelled

1994 ◽  
Vol 166 (1) ◽  
pp. 73-86 ◽  
Author(s):  
Daniel J. Sussman ◽  
John Klingensmith ◽  
Patricia Salinas ◽  
Pamela S. Adams ◽  
Roel Nusse ◽  
...  
Keyword(s):  
Mouse Homolog ◽  
Isolation And Characterization ◽  
Segment Polarity ◽  
Segment Polarity Gene ◽  
Polarity Gene

scholarly journals Characterization of Suppressor of fused, a complete suppressor of the fused segment polarity gene of Drosophila melanogaster.

Genetics ◽  
1992 ◽  
Vol 132 (3) ◽  
pp. 725-736 ◽  
Author(s):  
T Préat
Keyword(s):  
Drosophila Melanogaster ◽  
Maternal Effect ◽  
Opposite Effect ◽  
Loss Of Function ◽  
Suppressor Of Fused ◽  
Segment Polarity ◽  
New Gene ◽  
Segment Polarity Gene ◽  
Polarity Gene

Abstract fused (fu) is a maternal effect segment polarity gene of Drosophila melanogaster. In addition, fu females have tumorous ovaries. Two ethyl methanesulfonate mutageneses were carried out in order to isolate suppressors of the fu phenotype. A new gene, Suppressor of fused (Su(fu)), was identified. It is located in the 87C8 region of the third chromosome. Su(fu) displays a maternal effect and is also expressed later in development. Although Su(fu)LP is a complete loss-of-function mutation, it is homozygous viable and has no phenotype by itself. Su(fu) fully suppresses the embryonic and adult phenotypes of fu mutants. Su(fu) mutations are semidominant and a Su(fu)+ duplication has an opposite effect, enhancing the fused phenotype. It is proposed therefore that the Su(fu)+ product is involved in the same developmental step as the Fu+ kinase. Thus, a new gene interacting with the segment polarity pathway was identified using an indirect approach.

scholarly journals Cloning and characterization of the segment polarity gene cubitus interruptus Dominant of Drosophila.

1990 ◽  
Vol 4 (6) ◽  
pp. 1053-1067 ◽  
Author(s):  
T V Orenic ◽  
D C Slusarski ◽  
K L Kroll ◽  
R A Holmgren
Keyword(s):  
Cubitus Interruptus ◽  
Segment Polarity ◽  
Segment Polarity Gene ◽  
Polarity Gene

Positional signaling by hedgehog in Drosophila imaginal disc development

Development ◽  
1995 ◽  
Vol 121 (1) ◽  
pp. 1-10 ◽  
Author(s):  
A.L. Felsenfeld ◽  
J.A. Kennison
Keyword(s):  
Hedgehog Signaling ◽  
Imaginal Disc ◽  
Ectopic Expression ◽  
Posterior Compartment ◽  
Anterior Compartment ◽  
Segment Polarity ◽  
Wing Structures ◽  
Wing Discs ◽  
Segment Polarity Gene ◽  
Polarity Gene

We describe a dominant gain-of-function allele of the segment polarity gene hedgehog. This mutation causes ectopic expression of hedgehog mRNA in the anterior compartment of wing discs, leading to overgrowth of tissue in the anterior of the wing and partial duplication of distal wing structures. The posterior compartment of the wing is unaffected. Other imaginal derivatives are affected, resulting in duplications of legs and antennae and malformations of eyes. In mutant imaginal wing discs, expression of the decapentaplegic gene, which is implicated in the hedgehog signaling pathway, is also perturbed. The results suggest that hedgehog protein acts in the wing as a signal to instruct neighboring cells to adopt fates appropriate to the region of the wing just anterior to the compartmental boundary.

rasp, a putative transmembrane acyltransferase, is required for Hedgehog signaling

Development ◽  
2002 ◽  
Vol 129 (4) ◽  
pp. 843-851 ◽  
Author(s):  
Craig A. Micchelli ◽  
Inge The ◽  
Erica Selva ◽  
Vladic Mogila ◽  
Norbert Perrimon
Keyword(s):  
Hedgehog Signaling ◽  
Transmembrane Protein ◽  
Post Translational Modification ◽  
Protein Levels ◽  
Lethal Mutations ◽  
Segment Polarity ◽  
Invertebrate Development ◽  
Membrane Bound ◽  
Segment Polarity Gene ◽  
Polarity Gene

Members of the Hedgehog (Hh) family encode secreted molecules that act as potent organizers during vertebrate and invertebrate development. Post-translational modification regulates both the range and efficacy of Hh protein. One such modification is the acylation of the N-terminal cysteine of Hh. In a screen for zygotic lethal mutations associated with maternal effects, we have identified rasp, a novel Drosophila segment polarity gene. Analysis of the rasp mutant phenotype, in both the embryo and wing imaginal disc demonstrates that rasp does not disrupt Wnt/Wingless signaling but is specifically required for Hh signaling. The requirement of rasp is restricted only to those cells that produce Hh; hh transcription, protein levels and distribution are not affected by the loss of rasp. Molecular analysis reveals that rasp encodes a multipass transmembrane protein that has homology to a family of membrane bound O-acyl transferases. Our results suggest that Rasp-dependent acylation is necessary to generate a fully active Hh protein.

Transcription of the segment-polarity gene wingless in the imaginal discs of Drosophila, and the phenotype of a pupal-lethal wg mutation

Development ◽  
1988 ◽  
Vol 102 (3) ◽  
pp. 489-497 ◽  
Author(s):  
N.E. Baker
Keyword(s):  
In Situ Hybridization ◽  
Imaginal Discs ◽  
Apical Cytoplasm ◽  
Segment Polarity ◽  
Disc Cells ◽  
Segment Polarity Gene ◽  
Drosophila Embryos ◽  
Polarity Gene

Wingless (wg) is a segment-polarity gene in Drosophila which is related to the murine proto-oncogene int1. In Drosophila embryos, wg transcription defines part of each parasegment. In situ hybridization shows that wg is also expressed in the imaginal discs which give rise to the adult during metamorphosis. Transcripts are localized in the apical cytoplasm of disc cells, and accumulate in different patterns in dorsal and ventral discs. The wgCX3 mutation produces morphological defect in the adult structures derived from these imaginal discs. The results show that wg is involved in the development of the adult, as well as the embryo, but that the imaginal discs do not express this segment-polarity gene in an identical pattern to the embryonic segments.

The segment polarity gene armadillo interacts with the wingless signaling pathway in both embryonic and adult pattern formation

Development ◽  
1991 ◽  
Vol 111 (4) ◽  
pp. 1029-1043 ◽  
Author(s):  
M. Peifer ◽  
C. Rauskolb ◽  
M. Williams ◽  
B. Riggleman ◽  
E. Wieschaus
Keyword(s):  
Pattern Formation ◽  
Imaginal Discs ◽  
Protein Accumulation ◽  
Segment Polarity Genes ◽  
Adult Pattern ◽  
Segment Polarity ◽  
Lethal Allele ◽  
Segment Polarity Gene ◽  
Polarity Gene

The segment polarity genes of Drosophila were initially defined as genes required for pattern formation within each embryonic segment. Some of these genes also function to establish the pattern of the adult cuticle. We have examined the role of the armadillo (arm) gene in this latter process. We confirmed and extended earlier findings that arm and the segment polarity gene wingless are very similar in their effects on embryonic development. We next discuss the role of arm in pattern formation in the imaginal discs, as determined by using a pupal lethal allele, by analyzing clones of arm mutant tissue in imaginal discs, and by using a transposon carrying arm to produce adults with a reduced level of arm. Together, these experiments established that arm is required for the development of all imaginal discs. The requirement for arm varies along the dorsal-ventral and proximal-distal axes. Cells that require the highest levels of arm are those that express the wingless gene. Further, animals with reduced arm levels have phenotypes that resemble those of weak alleles of wingless. We present a description of the patterns of arm protein accumulation in imaginal discs. Finally, we discuss the implications of these results for the role of arm and wingless in pattern formation.

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