scholarly journals Karyotype analysis and visualization of 45S rRNA genes using fluorescence in situ hybridization in aroids (Araceae)

2015 ◽  
Vol 9 (2) ◽  
pp. 145-160 ◽  
Author(s):  
Katrijn Van Laere ◽  
Prabhu Shankar Lakshmanan ◽  
Tom Eeckhaut ◽  
Johan Van Huylenbroeck ◽  
Erik Van Bockstaele ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Karyotype Analysis ◽  
Rrna Genes

Karyotype analysis of Oreochromis mossambicus, O. urolepis hornorum and their hybrid based on Cot-1 DNA bands by fluorescence in situ hybridization

2010 ◽  
Vol 42 (8) ◽  
pp. 1178-1185 ◽  
Author(s):  
Hua Ping Zhu ◽  
Mai Xin Lu ◽  
Zhang Han Huang ◽  
Feng Ying Gao ◽  
Dong Mei Ma ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Karyotype Analysis ◽  
Oreochromis Mossambicus

Karyotype analysis using FISH (fluorescence in situ hybridization) in Fragaria

2012 ◽  
Vol 136 ◽  
pp. 95-100 ◽  
Author(s):  
Il Rae Rho ◽  
Yoon Jung Hwang ◽  
Hyung Il Lee ◽  
Choon-Hwan Lee ◽  
Ki Byung Lim
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Karyotype Analysis

Conventional Karyotype and Fluorescence in situ Hybridization (FISH) Technology

2019 ◽  
Author(s):  
Janet M. Cowan
Keyword(s):  
In Situ Hybridization ◽  
High Resolution ◽  
Fluorescence In Situ Hybridization ◽  
Dna Analysis ◽  
Karyotype Analysis ◽  
Hybridization Probe ◽  
Clinical Syndromes ◽  
Degree Of Condensation ◽  
Very High

Karyotype analysis of cells has been in use for many years and has led to the causative genetic change in numerous clinical syndromes, including trisomy 21, Klinefelter, Turner, Prader-Willi and Angelman syndromes. The resolution of the test depends on the degree of condensation of the chromosomes in the karyotype, but even at high resolution (> 800 bands per haploid set) the changes identified are in the order of 5 Mb of DNA.  Fluorescence in situ hybridization (FISH) bridges the gap between the relatively low resolution of karyotype analysis and the very high resolution of DNA analysis. With FISH it is possible to identify smaller changes in individual cells. The size of the change identified correlates with the size of the probe, which vary from 120 kb to 600 kb in size. FISH is widely used to confirm deletions or duplications identified by newer methods, such as array analysis.   This review contains 8 figures, 3 tables, and 25 references. Keywords: Cytogenetics, chromosome, karyotype, chromosomal resolution, tissue culture, fluorescence, hybridization, probe

Physical mapping of 45S rRNA genes in Cucumis species by fluorescence in situ hybridization

1999 ◽  
Vol 77 (3) ◽  
pp. 389-393 ◽  
Author(s):  
Jin-Feng Chen ◽  
Jack E Staub ◽  
Jeffrey W Adelberg ◽  
Jiming Jiang
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Cucumis Sativus ◽  
Rrna Genes ◽  
Phylogenetic Distance ◽  
Rdna Sites ◽  
Cucumis Species ◽  
Cucumis Hystrix ◽  
26S Rrna

The chromosomal locations of the genes coding for the 18S-5.8S-26S rRNA was investigated in Cucumis species using fluorescence in situ hybridization. Cucumber (Cucumis sativus L., 2n = 2x = 14) possesses four pairs of rDNA loci that were mapped to chromosomes 1C, 2C, 4C, and 7C. The distinctive hybridization sites of the 18S-5.8S-26S rRNA genes provide several useful cytogenetic markers for identification of chromosomes in C. sativus. The 18S-5.8S-26S rDNA genes have also been detected on two chromosome pairs, one major and one minor pair of loci, in melon (Cucumis melo L., 2n = 2x = 24) and on three pairs of Cucumis hystrix Chakr. chromosomes. The different number and pattern of rDNA sites is consistent with the hypothesis that considerable phylogenetic distance exists among these species.Key words: fluorescence in situ hybridization, 45S rRNA gene, cytogenetics, Cucumis sativus, Cucucmis melo, Cucumis hystrix.

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