Quantitation of Cytokine mRNA Expression in Cobra Snake Venom Stimulated PBMC of Horses Using Real-Time RT-PCR

2015 ◽  
Vol 9 (6) ◽  
pp. 412-419
Author(s):  
Surasak Akesowan ◽  
Sunutcha Suntrarach ◽  
Suchitra Khunsap ◽  
Wichit Thaveekarn
Keyword(s):  
Mrna Expression ◽  
Real Time ◽  
Snake Venom ◽  
Rt Pcr ◽  
Cytokine Mrna ◽  
Cytokine Mrna Expression

Measurement of cytokine mRNA expression in intestinal biopsies of cats with inflammatory enteropathy using quantitative real-time RT-PCR

2006 ◽  
Vol 113 (3-4) ◽  
pp. 404-414 ◽  
Author(s):  
N. Nguyen Van ◽  
K. Taglinger ◽  
C.R. Helps ◽  
S. Tasker ◽  
T.J. Gruffydd-Jones ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Real Time ◽  
Rt Pcr ◽  
Cytokine Mrna ◽  
Cytokine Mrna Expression

scholarly journals Heterophils isolated from chickens resistant to extra-intestinal Salmonella enteritidis infection express higher levels of pro-inflammatory cytokine mRNA following infection than heterophils from susceptible chickens

2004 ◽  
Vol 132 (6) ◽  
pp. 1029-1037 ◽  
Author(s):  
PAMELA J. FERRO ◽  
CHRISTINA L. SWAGGERTY ◽  
PETE KAISER ◽  
IGAL Y. PEVZNER ◽  
MICHAEL H. KOGUT
Keyword(s):  
Mrna Expression ◽  
Real Time ◽  
Salmonella Enteritidis ◽  
Inflammatory Cytokine ◽  
Rt Pcr ◽  
Cytokine Mrna ◽  
Reciprocal Crosses ◽  
First Report ◽  
Cytokine Mrna Expression

Previous studies showed differences in in vitro heterophil function between parental (A>B) broilers and F1 reciprocal crosses (D>C). Our objectives were to (1) determine if in vitro variations translate to differences in resistance to Salmonella enteritidis (SE) and (2) quantitate cytokine mRNA in heterophils from SE-infected chicks. One-day-old chicks were challenged and organs were cultured for SE. Chicks with efficient heterophils (A and D) were less susceptible to SE compared to chicks with inefficient heterophils (B and C). Heterophils were isolated from SE-infected chicks and cytokine mRNA expression was evaluated using quantitative real-time RT–PCR. Pro-inflammatory cytokine mRNA was up-regulated in heterophils from SE-resistant chicks compared to susceptible chicks. This is the first report to quantitate cytokine mRNA in heterophils from SE-infected chicks. These data show a relationship between in vitro heterophil function, increased pro-inflammatory cytokine mRNA expression, and increased resistance to SE in 1-day-old chicks.

scholarly journals A Study of Cytokine in Palatine Tonsil. Cytokine mRNA Expression Determined by RT-PCR.

1999 ◽  
Vol 102 (2) ◽  
pp. 254-264 ◽  
Author(s):  
Junichi Wakashima ◽  
Yasuaki Harabuchi ◽  
Hideaki Shirasaki
Keyword(s):  
Mrna Expression ◽  
Palatine Tonsil ◽  
Rt Pcr ◽  
Cytokine Mrna ◽  
Cytokine Mrna Expression

Quantification of cytokine mRNA expression by RT PCR in samples of previously frozen blood

1997 ◽  
Vol 210 (2) ◽  
pp. 195-203 ◽  
Author(s):  
Niels Kruse ◽  
Martin Pette ◽  
Klaus Toyka ◽  
Peter Rieckmann
Keyword(s):  
Mrna Expression ◽  
Rt Pcr ◽  
Cytokine Mrna ◽  
Cytokine Mrna Expression
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