Plant Regeneration from Nodal Segments Derived Callus in Hemidesmus indicus (L.) R. Br (Anantamul) an Endangered Medicinal Plant in Bangladesh

An efficient in vitro propagation of an endangered medicinal plant Vanasushava pedata (Apiaceae) by axillary shoot proliferation from nodal segments of mature plants was designed. The medium type and growth regulators markedly influenced in vitro regeneration of V. pedata. An in vitro plantlet production system has been investigated on MS with the synergistic combination of BA (5.0 mg/l), IAA (0.1 mg/l) and 3 % sucrose which promoted the maximum number of shoots (8.6) as well as enhanced shoot lengths. Subculturing of nodal segments from in vitro derived shoots on a similar medium enabled continuous production of healthy shoots with a similar frequency. Rooting was highest (100%) on half strength MS containing IAA (2.0 mg/l). Micropropagated plants established in garden soil and forest humus (1 : 1) were uniform and identical to the donor plants with respect of growth characteristics as well as floral features. These in vitro-raised plants grew normally in greenhouse and natural habitat without showing any morphological variation. Â Key words: Vanasushava pedata, Medicinal plant, Nodal explants, Micropropagation, Successful acclimationDOI = 10.3329/ptcb.v16i2.1109Plant Tissue Cult. & Biotech. 16(2): 85-94, 2006 (December)

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Somatic Embryogenesis and Plant Regeneration in Gloriosa superba L.: An Endangered Medicinal Plant

In vitro Plant Breeding towards Novel Agronomic Traits ◽

10.1007/978-981-32-9824-8_2 ◽

2019 ◽

pp. 27-42

Author(s):

Venkatachalam Balamurugan ◽

Thomas Cheeran Amal ◽

Palanisamy Karthika ◽

Subramanian Selvakumar ◽

Krishnan Vasanth

Keyword(s):

Somatic Embryogenesis ◽

Plant Regeneration ◽

Medicinal Plant ◽

Gloriosa Superba ◽

Endangered Medicinal Plant

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A MICROPROPAGATION TECHNIQUE OF HEMIDESMUS INDICUS (ASCLEPIADACEAE),A VALUABLE MEDICINAL PLANT

FLORA AND FAUNA ◽

10.33451/florafauna.v24i1pp16-20 ◽

2018 ◽

Vol 24 (1) ◽

Cited By ~ 2

Author(s):

SUSANTA KUMAR MAITY

Keyword(s):

Medicinal Plant ◽

Leaf Shape ◽

Basal Medium ◽

Nodal Segments ◽

Nodal Segment ◽

Hemidesmus Indicus ◽

Regenerated Plants ◽

Nodular Callus ◽

In Vitro Clonal Propagation

An efficient protocol was established for in vitro clonal propagation of Hemidesmus indicus (Anantamul) belongs to the family Asclepiadaceae, a widely used medicinal plant through callus culture in using nodal segment. Yellowish nodular callus was observed from nodal segments on MS basal medium supplemented with 0.5 mg/L BAP + 0.2 mg/L NAA within four weeks of culture. Large number of shoots (11.4±0.2) and roots (8.2±0.4) were obtained when the callus was sub cultured on MS medium with 0.2 mg/L BAP. The regenerated plantlets were acclimatized by transferring them to soil. The survival rate of plantlets was found to be 90%. Regenerated plants were morphologically comparable having normal leaf shape and growth.

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