Characterization of Protease Activity of Lactococcus lactis Species Isolated from Raw Camel’s Milk

2016 ◽  
Vol 16 (6) ◽  
pp. 215-220 ◽  
Author(s):  
N. Addi ◽  
B. Guessas
Keyword(s):  
Lactococcus Lactis ◽  
Protease Activity ◽  
Camel's Milk

Location and characterization of aminopeptidase N in Lactococcus lactis subsp. cremoris HP

1992 ◽  
Vol 37 (1) ◽  
pp. 46-54 ◽  
Author(s):  
Fred A. Exterkate ◽  
Marian de Jong ◽  
Gerrie J. C. M. de Veer ◽  
Ronald Baankreis
Keyword(s):  
Lactococcus Lactis ◽  
Aminopeptidase N ◽  
Lactococcus Lactis Subsp

scholarly journals Discrepancies between the phenotypic and genotypic characterization of Lactococcus lactis cheese isolates

2006 ◽  
Vol 43 (6) ◽  
pp. 637-644 ◽  
Author(s):  
M. de la Plaza ◽  
A. Rodríguez ◽  
P. Fernández de Palencia ◽  
M.C. Martínez-Cuesta ◽  
C. Peláez ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Genotypic Characterization ◽  
Phenotypic And Genotypic Characterization

Identification and characterization of glutamate dehydrogenase activity in wild Lactococcus lactis isolated from raw milk cheeses

2017 ◽  
Vol 244 (4) ◽  
pp. 603-609 ◽  
Author(s):  
Luz P. Gómez de Cadiñanos ◽  
Carmen Peláez ◽  
M. Carmen Martínez-Cuesta ◽  
Tomás García-Cayuela ◽  
Teresa Requena
Keyword(s):  
Glutamate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Lactococcus Lactis ◽  
Raw Milk ◽  
Glutamate Dehydrogenase Activity ◽  
Identification And Characterization

scholarly journals Characterization of Multiple Regions Involved in Replication and Mobilization of Plasmid pNZ4000 Coding for Exopolysaccharide Production in Lactococcus lactis

1998 ◽  
Vol 180 (20) ◽  
pp. 5285-5290 ◽  
Author(s):  
Richard van Kranenburg ◽  
Willem M. de Vos
Keyword(s):  
Lactococcus Lactis ◽  
Insertion Sequence ◽  
Inverted Repeats ◽  
Conjugative Transfer ◽  
Exopolysaccharide Production ◽  
Naturally Occurring ◽  
Gene Coding ◽  
Multiple Regions ◽  
Plasmid R64

ABSTRACT We characterized the regions involved in replication and mobilization of the 40-kb plasmid pNZ4000, encoding exopolysaccharide (EPS) production in Lactococcus lactis NIZO B40. The plasmid contains four highly conserved replication regions with homologous rep genes (repB1, repB2,repB3, and repB4) that belong to the lactococcal theta replicon family. Subcloning of each replicon individually showed that all are functional and compatible in L. lactis. Plasmid pNZ4000 and genetically labeled derivatives could be transferred to different L. lactis strains by conjugation, and pNZ4000 was shown to be a mobilization plasmid. Two regions involved in mobilization were identified near two of the replicons; both included an oriT sequence rich in inverted repeats. Conjugative mobilization of the nonmobilizable plasmid pNZ124 was promoted by either one of these oriT sequences, demonstrating their functionality. One oriT sequence was followed by a mobA gene, coding for atrans-acting protein, which increased the frequency of conjugative transfer 100-fold. The predicted MobA protein and theoriT sequences show protein and nucleotide similarity, respectively, with the relaxase and with the inverted repeat andnic site of the oriT from the Escherichia coli plasmid R64. The presence on pNZ4000 of four functional replicons, two oriT sequences, and several insertion sequence-like elements strongly suggests that this EPS plasmid is a naturally occurring cointegrate.

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