Submerged Monoxenic Culture Medium Development for Heterorhabditis bacteriophora and its Symbiotic Bacterium Photorhabdus luminescens: Protein Sources

2011 ◽  
Vol 21 (8) ◽  
pp. 869-873 ◽  
Author(s):  
Chun-Hwi Cho
Keyword(s):  
Culture Medium ◽  
Symbiotic Bacterium ◽  
Heterorhabditis Bacteriophora ◽  
Monoxenic Culture ◽  
Photorhabdus Luminescens ◽  
Protein Sources ◽  
Medium Development ◽  
Culture Medium Development

Using Genomic Tools for the Identification of Important Signaling Pathways in Order to Facilitate Cell Culture Medium Development

2007 ◽  
pp. 591-593 ◽  
Author(s):  
Daniel W. Allison ◽  
Kathryn A. Aboytes ◽  
Terrell K. Johnson ◽  
Danny K. Fong ◽  
Stacy L. Leugers ◽  
...  
Keyword(s):  
Cell Culture ◽  
Signaling Pathways ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Medium Development ◽  
Genomic Tools ◽  
Culture Medium Development

Physico-chemical properties and mode of action of a signal from the symbiotic bacterium Photorhabdus luminescens inducing dauer juvenile recovery in the entomopathogenic nematode Heterorhabditis bacteriophora

Nematology ◽  
2001 ◽  
Vol 3 (8) ◽  
pp. 849-853 ◽  
Author(s):  
Ralf-Udo Ehlers ◽  
Jens Aumann
Keyword(s):  
Entomopathogenic Nematodes ◽  
Entomopathogenic Nematode ◽  
Chemical Properties ◽  
Symbiotic Bacterium ◽  
Heterorhabditis Bacteriophora ◽  
Juvenile Stage ◽  
Food Sources ◽  
Symbiotic Bacteria ◽  
Photorhabdus Luminescens ◽  
Physico Chemical

AbstractRecovery in entomopathogenic nematodes is the exit from the dauer juvenile stage. It is a response to environmental queues signalling the presence of food sources (e.g., insect haemolymph). The bacterium Photorhabdus luminescens excretes a signal which also induces recovery of its symbiotic Heterorhabditis bacteriophora dauer juveniles. This bacterial signal is composed of at least two compounds with different polarity. The symbiotic bacteria also secrete an antagonistic signal which inhibits nematode recovery. The recovery-inducing signal compounds have a molecular mass of less than 20 kDa and are negatively charged. The data indicate that at least one compound is smaller than 5 kDa. The bacterial signal triggers by receptor binding, the first step in a recovery-inducing muscarinic signalling pathway.

scholarly journals Culture Medium Development for Microbial-Derived Surfactants Production—An Overview

Molecules ◽  
2018 ◽  
Vol 23 (5) ◽  
pp. 1049 ◽  
Author(s):  
Abdul Nurfarahin ◽  
Mohd Mohamed ◽  
Lai Phang
Keyword(s):  
Culture Medium ◽  
Medium Development ◽  
Culture Medium Development

scholarly journals Culture medium development for lactobacillus acidophilus encapsulation

2015 ◽  
Vol 5 (11(77)) ◽  
pp. 17
Author(s):  
Валентина Леонтіївна Большакова ◽  
Наталія Вячеславівна Кондратюк ◽  
Євген Павлович Пивоваров
Keyword(s):  
Lactobacillus Acidophilus ◽  
Culture Medium ◽  
Medium Development ◽  
Culture Medium Development

scholarly journals Characterization of Photorhabdus luminescens Growth for the Rearing of the Beneficial Nematode Heterorhabditis bacteriophora

2011 ◽  
Vol 52 (3) ◽  
pp. 325-331 ◽  
Author(s):  
Sunita Singh ◽  
Moreau Eric ◽  
Inman Floyd ◽  
Holmes D. Leonard
Keyword(s):  
Heterorhabditis Bacteriophora ◽  
Photorhabdus Luminescens

Influence of bacterial density and mating on life history traits of Heterorhabditis bacteriophora

Nematology ◽  
2016 ◽  
Vol 18 (8) ◽  
pp. 963-972 ◽  
Author(s):  
Temesgen Addis ◽  
Shiferaw Demissie ◽  
Olaf Strauch ◽  
Ralf-Udo Ehlers
Keyword(s):  
Life History ◽  
Life History Traits ◽  
Insect Pests ◽  
Heterorhabditis Bacteriophora ◽  
The Body ◽  
Bacterial Density ◽  
Photorhabdus Luminescens ◽  
Hanging Drop ◽  
Offspring Production ◽  
Drop Technique

Heterorhabditis bacteriophora, associated with Photorhabdus luminescens, is commonly used against insect pests. Dauer juveniles (DJ) develop into self-fertilising hermaphrodites that lay eggs until juveniles hatch inside the uterus and feed on the body content of the mother (endotokia matricida). The life history traits of H. bacteriophora were studied at 2.5 × 109, 5 × 109, 10 × 109 and 20 × 109 cells ml−1 of P. luminescens at 25°C using a hanging drop technique. The number of offspring produced per hermaphrodite increased from 50 at 2.5 × 109 cells ml−1 to 269 at 20 × 109 cells ml−1 of P. luminescens. The bacterial density did not influence the beginning of endotokia matricida, hermaphrodite death, DJ release from the maternal carcass and the percentage of juveniles obtained through endotokia matricida. Mating of automictic females could not increase offspring production and survival. Endotokia matricida is an obligatory developmental step in H. bacteriophora.

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