scholarly journals Cutting Edge: Cell Surface Expression and Lipopolysaccharide Signaling Via the Toll-Like Receptor 4-MD-2 Complex on Mouse Peritoneal Macrophages

2000 ◽  
Vol 164 (7) ◽  
pp. 3471-3475 ◽  
Author(s):  
Sachiko Akashi ◽  
Rintaro Shimazu ◽  
Hirotaka Ogata ◽  
Yoshinori Nagai ◽  
Kiyoshi Takeda ◽  
...  
Keyword(s):  
Cell Surface ◽  
Surface Expression ◽  
Peritoneal Macrophages ◽  
Cutting Edge ◽  
Cell Surface Expression ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Mouse Peritoneal Macrophages

CD14+ Cell-Surface Expression of Toll-like Receptor 4 is Lower in Exercise Trained Older Women

2004 ◽  
Vol 36 (Supplement) ◽  
pp. S149???S150
Author(s):  
Brian K. McFarlin ◽  
Mike G. Flynn ◽  
Wayne W. Campbell ◽  
Laura K. Stewart ◽  
Kyle L. Timmerman ◽  
...  
Keyword(s):  
Cell Surface ◽  
Older Women ◽  
Surface Expression ◽  
Cell Surface Expression ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4

CD14+ Cell-Surface Expression of Toll-like Receptor 4 is Lower in Exercise Trained Older Women

2004 ◽  
Vol 36 (Supplement) ◽  
pp. S149-S150
Author(s):  
Brian K. McFarlin ◽  
Mike G. Flynn ◽  
Wayne W. Campbell ◽  
Laura K. Stewart ◽  
Kyle L. Timmerman ◽  
...  
Keyword(s):  
Cell Surface ◽  
Older Women ◽  
Surface Expression ◽  
Cell Surface Expression ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4

scholarly journals MD-2 Is Necessary for the Toll-Like Receptor 4 Protein To Undergo Glycosylation Essential for Its Translocation to the Cell Surface

2003 ◽  
Vol 10 (3) ◽  
pp. 405-410 ◽  
Author(s):  
Takahiro Ohnishi ◽  
Masashi Muroi ◽  
Ken-ichi Tanamoto
Keyword(s):  
Cell Surface ◽  
Surface Expression ◽  
Single Band ◽  
Cell Surface Expression ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Cellular Extract ◽  
Molecular Masses

ABSTRACT MD-2 has been reported to be required for the translocation of the Toll-like receptor 4 (TLR4) to the cell surface. However, the mechanism by which MD-2 promotes TLR4 translocation is unknown. We identified the presence of two forms of TLR4 with different molecular masses (approximately 110 and 130 kDa) when TLR4 was expressed together with MD-2. Expressing TLR4 alone produced only the 110-kDa form. Using a membrane-impermeable biotinylation reagent, we found that only the 130-kDa form of TLR4 was expressed on the cell surface. When a cellular extract prepared from cells expressing TLR4 and MD-2 was treated with N-glycosidase, the two forms of TLR4 converged into a single band whose size was smaller than the 110-kDa form of TLR4. Mutation of TLR4 at Asn526 or Asn575 resulted in the disappearance of the 130-kDa form and prevented TLR4 from being expressed on the cell surface without affecting the ability of TLR4 to associate with MD-2. These results indicate that TLR4 is able to undergo multiple glycosylations without MD-2 but that the specific glycosylation essential for cell surface expression requires the presence of MD-2.

scholarly journals A Protein Associated with Toll-Like Receptor 4 (PRAT4A) Regulates Cell Surface Expression of TLR4

2006 ◽  
Vol 177 (3) ◽  
pp. 1772-1779 ◽  
Author(s):  
Yasutaka Wakabayashi ◽  
Makiko Kobayashi ◽  
Sachiko Akashi-Takamura ◽  
Natsuko Tanimura ◽  
Kazunori Konno ◽  
...  
Keyword(s):  
Cell Surface ◽  
Surface Expression ◽  
Cell Surface Expression ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4

A molecule that is associated with Toll-like receptor 4 and regulates its cell surface expression

2006 ◽  
Vol 339 (4) ◽  
pp. 1076-1082 ◽  
Author(s):  
Kazunori Konno ◽  
Yasutaka Wakabayashi ◽  
Sachiko Akashi-Takamura ◽  
Takashi Ishii ◽  
Makiko Kobayashi ◽  
...  
Keyword(s):  
Cell Surface ◽  
Surface Expression ◽  
Cell Surface Expression ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4
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