Purification and characterization of alkaline protease produced by Streptomyces flavogriseus and its application as a biocontrol agent for plant pathogens

2019 ◽  
Vol 18 (4) ◽  
pp. 332
Author(s):  
ElsayedE Mostafa ◽  
MoatazaM Saad ◽  
HelmyM Hassan ◽  
ElsayedHussien Zeidan
Keyword(s):  
Alkaline Protease ◽  
Plant Pathogens ◽  
Biocontrol Agent ◽  
Purification And Characterization

Purification and characterization of an alkaline protease from Oerskovia xanthineolytica TK-1

1994 ◽  
Vol 77 (5) ◽  
pp. 554-556 ◽  
Author(s):  
Kazuo Saeki ◽  
Junko Iwata ◽  
Yasuo Watanabe ◽  
Youichi Tamai
Keyword(s):  
Alkaline Protease ◽  
Purification And Characterization

One-step purification and characterization of an alkaline protease from haloalkaliphilic Bacillus sp.

2005 ◽  
Vol 1075 (1-2) ◽  
pp. 103-108 ◽  
Author(s):  
Anshu Gupta ◽  
Ipsita Roy ◽  
R.K. Patel ◽  
S.P. Singh ◽  
S.K. Khare ◽  
...  
Keyword(s):  
Alkaline Protease ◽  
Bacillus Sp ◽  
Purification And Characterization ◽  
One Step

scholarly journals Purification and Characterization of Chitinase from the Nematode – Fungus Paecilomyces sp. P1

2019 ◽  
Vol 35 (1) ◽  
Author(s):  
Chu Thanh Binh ◽  
Nguyen Phuong Nhue ◽  
Ho Tuyen ◽  
Bui Thi Viet Ha
Keyword(s):  
Metarhizium Anisopliae ◽  
Biocontrol Agent ◽  
Specific Activity ◽  
Chitinase Activity ◽  
Paecilomyces Lilacinus ◽  
Purification And Characterization ◽  
Crinipellis Perniciosa ◽  
Isolation And Characterization ◽  
Invertebr Pathol

The nematophagous – fungi Paecilomyces sp. is curently developed as a biocontrol agent against plant parasitic nematodes (Khan et al., 2003; Yang et al., 2007). Biological control agents can infiltrate certain nematode sites and destroy them by producing some enzymes including chitinase (Khadijeh et al., 2017). The purpose of this study was to purify, determine the chitinase activity from Paecilomyces sp. P1. With Lugol reagent, chitinase of this strain was characterized by diffusion on agar plate. Chitinase specific activity was determined by measuring the release of reducing saccharides from colloidal chitin by the N-acetyl-glucosamine-dinitrosalicylate method at 540 nm. By using the saturated (NH4)2SO4 precipitation at 65% concentration, DEAE A-50 ion exchange chromatography and SDS - PAGE concentration 12.5%, chitinase molecules weigh nearly 50kDa, having a specific activity of 133,3 U/mg, 2,1-fold higher than that of supernatant. Furthermore, method of testing with the nematode Meloidogyne sp., the ability to kill nematodes of Paecilomyces sp. P1 reached 58% efficiency in 96h. These results were a scientific basis for the application of Paecilomyces sp. P1 in the production of nematode insecticides. Keywords Paecilomyces sp. P1; chitinase; purify, biocontrol, Meloidogyne sp References   [1] Nguyễn Ngọc Châu, Tuyến trùng thực vật và cơ sở phòng trừ, NXBKHKTHN, 2003.[2] Nguyễn Hữu Quân, Vũ Văn Hạnh, Quyền Đình Thi, Phạm Thị Huyền, Tinh sạch và đánh giá tính chất lý hóa của chitinase từ nấm Lecanicillium lecanii, Kỷ yếu Hội nghị Công nghệ Sinh học toàn quốc, 1 (2013) 426.[3] CM Baratto, V Dutra, JT Boldo, LB Leiria, MH Vainstein, A. Schrank Isolation, characterization and transcriptional analysis of the chitinase chi2 gene (DQ011663) from the biocontrol fungus Metarhizium anisopliae var. anisopliae., Curr Microbiol, 53 (2006) 217.[4] D. Wharton,. Nematode eggshells, Parasitology 81 (1980) 447.[5] F. A. Zaki, D. 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Biotechnol. 64 (2003) 70.[9] JLD Marco , LHC Lima, MV Sousa MV, CR Felix A Trichoderma harzianum chitinase destroys the cell wall of the phytopathogen Crinipellis perniciosa, the causal agent of witches’ broomof cocoa, J Microbiol Biotechnol 16 (2000) 383.[10] Khan Alamgir, Williams Keith, Mark P. Molloy, and Nevalainen Henlena, Purification and characterization of a serine protease and chitinases from Paecilomyces lilacinus and detection of chitinase activity on 2D gels, Protein Expression and Purification 32 (2003) 210.[11] Khadijeh Abbsi, Doustmorad ZAFARI, Robert WICK., Evaluation of chitinase enzyme in fungal isolates obtained from golden potato cyst nematode (Globodera rostochiensis) Zemdirbyste-Agriculture, 2 (2017) 179.[12] Kopparapu Narasimha Kumar, Peng Zhou, Shuping Zhang, Qiaojuan Yan, Zhuqing Liu, Zhengqiang Jiang, Purification and characterization of a novel chitinase gene from Paecilomyces thermophila expressed in Escherichia coli. Carbonhydrate Reseach 347 (2012) 155.[13] Methanee Homthong, Anchanee Kubera, Matana Srihuttagum, Vipa Hongtrakul, Isolation and characterization of chitinase from soil fungi, Paecilomyces sp. Agriculture and Natural Resources, 1 (2016) 50.[14] RS Patil, V Ghormade, MV Desphande MV ,Chitinolytic enzymes: an exploration. Enzyme Microb. Technol. 26 (2002) 473[15] RJ Leger St , RM Cooper, AK Charnley, Characterization of chitinase and chitobiase produced by the entomopathogenic fungus Metarhizium anisopliae. J. Invertebr. Pathol. 58 (1991) 415.[16] S Leger, RJ Joshi RJ, RJ Bidochka, DW Roberts . Characterization and ultrastructural localization of Metarhizium anisopliae, M. xavoviride, and Beauveria bassiana during fungal invasion of host (Manduca sexta) cuticle. Appl Environ Microbiol 62 (1996)907.[17] SC Kang, S. Park, DG Lee ,, Purification and characterization of a novel chitinase from the entomopathogenic fungus, Metarhiziumanisopliae. J Invertebr Pathol., 73 (1999) 276.[18] P.J.M Bonants, P.F.L. Fitters, H. Thijs, E. den Belder, C. Waalwijk, J.W.D.M. Henfling. A basic serine protease from Paecilomyces lilacinus with biological activity against Meloidogyne hapla eggs, Microbiology 141(1995) 75.[19] VE Tikhonov, LV Lopez-Llorca, J Salinas, HB Jansson . Purification and characterization of chitinases from the nematophagous fungi Verticillium chlamydosporium and V. suchlasporium, Fungal Genet Biol (2002) 67[20] Van Nam Nguyen, YJ Kim, KT Oh, WJ Jung, RD Park , The antifungal activity of chitinases from Trichoderma aureoviride DY-59 and Rhizopus microsporus VS-9. Curr. Microbiol 56 (2008) 28.[21] Van Nam Nguyen, In-Jae Oh, Young-Ju Kim, Kil-Yong Kim, Young-Cheol Kim, Ro-Dong Par J Ind., Purification and characterization of chitinases from Paecilomyces variotii DG-3 parasitizing on Meloidogyne incognita eggs, (2009) 195[22] Z. Perveen and S. Shahzad S., , A comparative study of the efficacy of Paecilomyces species against root-knot nematode Meloidogyne incognita. Pakistan Journal of Nematology, 31 (2013) 125

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