Comparison of conventional and rapid methods for the detection of methicillin resistance in Staphylococcus aureus from blood cultures

2017 ◽  
Vol 20 (2) ◽  
pp. 196
Author(s):  
Ashwani Kumar ◽  
Bineeta Kashyap ◽  
Anurag Mehndiratta
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Blood Cultures ◽  
Rapid Methods

scholarly journals Evaluation of a Triplex PCR Assay To Discriminate Staphylococcus aureus from Coagulase-Negative Staphylococci and Determine Methicillin Resistance from Blood Cultures

2002 ◽  
Vol 40 (4) ◽  
pp. 1514-1517 ◽  
Author(s):  
N. Maes ◽  
J. Magdalena ◽  
S. Rottiers ◽  
Y. De Gheldre ◽  
M. J. Struelens
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Blood Cultures ◽  
Pcr Assay ◽  
Coagulase Negative Staphylococci

scholarly journals Molecular-genetic Method for Fast Direct Detection of Staphylococcus Aureus and Methicillin Resistance in Blood Cultures and Punctures

Folia Medica ◽  
2019 ◽  
Vol 61 (4) ◽  
pp. 559-565
Author(s):  
Raina T. Gergova ◽  
Virna-Maria S. Tsitou ◽  
Ivan G. Mitov
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Agents ◽  
Direct Detection ◽  
Methicillin Resistance ◽  
Molecular Genetic ◽  
Empirical Therapy ◽  
Blood Cultures ◽  
Coagulase Negative Staphylococci ◽  
Adequate Treatment ◽  
Genetic Method

Background: Invasive infections caused by methicillin resistant Staphylococcus aureus and coagulase-negative staphylococci (MRSA/MRSCoN) require fast, adequate treatment.&nbsp; The aim of this study was to develop a faster protocol for direct detection of MRSA/MRSCoN in blood cultures and in abscess punctures based on mecA and species specific identification of S. aureus by polymerase-chain reaction (PCR). Materials and methods: We examined 77 growth-positive BACTEC blood cultures and 50 abscess punctures by routine microbiological assay and simultaneous PCR detection of MRSA/MRSCoN. The speci&#64257;city of the PCR was evaluated by using DNA from another 15 microbial species for negative controls. We determined the minimum inhibitory concentration (MIC) of oxacillin, vancomycin, tigecycline, linezolid, levofloxacin, clindamycin, and erythromycin against the S. aureus isolates using the E-test.&nbsp; Results: In the blood cultures, the two methods detected 39.3% of MRSA, and 93.9% of MRCoNS. In the punctures, the PCR assay identified 20.9% of MRSA and 79.2% of MSSA. In the puncture cases, there were three PCR MRSA positive and culture negative samples. Screening for susceptibility to 14 antimicrobial agents demonstrated significantly higher (p<0.05) methicillin resistance in blood culture isolates than in the puncture ones (39.3% and 20.0%, respectively).&nbsp; Conclusion: The new PCR protocol was very fast and specific. It was more sensitive in detecting MRSA from abscess punctures than the routine microbiological techniques. This protocol will speed up the right choice of empirical therapy, which is extremely important for saving patients&rsquo; lives.

The Alteration of Methicillin Resistance in Staphylococcus aureus Strains Isolated from Blood Cultures Over Years

ANKEM Dergisi ◽  
2013 ◽  
Vol 27 (1) ◽  
pp. 38-42
Author(s):  
Yeliz Cetinkol ◽  
Fazilet Ozenc Cakir ◽  
Ozgur Enginyurt
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Blood Cultures

Healthcare-AssociatedStaphylococcus aureusBacteremia and the Risk for Methicillin Resistance: Is the Centers for Disease Control and Prevention Definition for Community-Acquired Bacteremia Still Appropriate?

2005 ◽  
Vol 26 (2) ◽  
pp. 204-209 ◽  
Author(s):  
Olivier Lesens ◽  
Yves Hansmann ◽  
Eimar Brannigan ◽  
Susan Hopkins ◽  
Pierre Meyer ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Disease Control ◽  
Methicillin Resistance ◽  
Blood Cultures ◽  
New Classification ◽  
Control And Prevention ◽  
Centers For Disease Control ◽  
Mrsa Strains ◽  
Healthcare Associated ◽  
Hospital Acquired

AbstractObjective:To evaluate a new classification for bloodstream infections that differentiates hospital acquired, healthcare associated, and community acquired in patients with blood cultures positive forStaphylococcus aureus.Design:Prospective, observational study.Setting:Three tertiary-care, university-affiliated hospitals in Dublin, Ireland, and Strasbourg, France.Patients:Two hundred thirty consecutive patients older than 18 years with blood cultures positive forS. aureus.Methods:S. aureusbacteremia (SAB) was defined as hospital acquired if the first positive blood culture was performed more than 48 hours after admission. Other SABs were classified as healthcare associated or community acquired according to the definition proposed by Friedman et al. When available, strains of methicillin-resistantStaphylococcus aureus(MRSA) were analyzed by pulsed-field gel electrophoresis (PFGE).Results:Eighty-two patients were considered as having community-acquired bacteremia according to the Centers for Disease Control and Prevention (CDC) classification. Of these 82 patients, 56% (46) had healthcare-associated SAB. MRSA prevalence was similar in patients with hospital-acquired and healthcare-associated SAB (41% vs 33%;P> .05), but significantly lower in the group with community-acquired SAB (11%;P< .03). PFGE of MRSA strains showed that most community-acquired and healthcare-associated MRSA strains were similar to hospital-acquired MRSA strains. On multivariate analysis, Friedman's classification was more effective than the CDC classification for predicting MRSA.Conclusion:These results support the call for a new classification for community-acquired bacteremia that would account for healthcare received outside the hospital by patients with SAB.

scholarly journals Evaluation of Three Rapid Methods for Detection of Methicillin Resistance in Staphylococcus aureus

2000 ◽  
Vol 38 (6) ◽  
pp. 2170-2173 ◽  
Author(s):  
L. Louie ◽  
S. O. Matsumura ◽  
E. Choi ◽  
M. Louie ◽  
A. E. Simor
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Rapid Methods

scholarly journals Clinical Impact of a PCR Assay for Identification of Staphylococcus aureus and Determination of Methicillin Resistance Directly from Blood Cultures

2003 ◽  
Vol 41 (8) ◽  
pp. 3942-3944 ◽  
Author(s):  
M. Hallin ◽  
N. Maes ◽  
B. Byl ◽  
F. Jacobs ◽  
Y. De Gheldre ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Blood Cultures ◽  
Clinical Impact ◽  
Pcr Assay

Evaluation of Three Rapid Methods for Detection of Methicillin Resistance in Staphylococcus aureus

2000 ◽  
Vol 38 (6) ◽  
pp. 2170-2173 ◽  
Author(s):  
L. Louie ◽  
S. O. Matsumura ◽  
E. Choi ◽  
M. Louie ◽  
A. E. Simor
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Rapid Methods
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