scholarly journals Evaluation of BD MAX Staph SR Assay for Differentiating Between Staphylococcus aureus and Coagulase-Negative Staphylococci and Determining Methicillin Resistance Directly From Positive Blood Cultures

2017 ◽  
Vol 37 (1) ◽  
pp. 39-44 ◽  
Author(s):  
Jaewoong Lee ◽  
Yeon-Joon Park ◽  
Dong-Jin Park ◽  
Kang-Gyun Park ◽  
Hae Kyung Lee
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Blood Cultures ◽  
Coagulase Negative Staphylococci

scholarly journals Evaluation of a Triplex PCR Assay To Discriminate Staphylococcus aureus from Coagulase-Negative Staphylococci and Determine Methicillin Resistance from Blood Cultures

2002 ◽  
Vol 40 (4) ◽  
pp. 1514-1517 ◽  
Author(s):  
N. Maes ◽  
J. Magdalena ◽  
S. Rottiers ◽  
Y. De Gheldre ◽  
M. J. Struelens
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Blood Cultures ◽  
Pcr Assay ◽  
Coagulase Negative Staphylococci

scholarly journals Molecular-genetic Method for Fast Direct Detection of Staphylococcus Aureus and Methicillin Resistance in Blood Cultures and Punctures

Folia Medica ◽  
2019 ◽  
Vol 61 (4) ◽  
pp. 559-565
Author(s):  
Raina T. Gergova ◽  
Virna-Maria S. Tsitou ◽  
Ivan G. Mitov
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Agents ◽  
Direct Detection ◽  
Methicillin Resistance ◽  
Molecular Genetic ◽  
Empirical Therapy ◽  
Blood Cultures ◽  
Coagulase Negative Staphylococci ◽  
Adequate Treatment ◽  
Genetic Method

Background: Invasive infections caused by methicillin resistant Staphylococcus aureus and coagulase-negative staphylococci (MRSA/MRSCoN) require fast, adequate treatment.&nbsp; The aim of this study was to develop a faster protocol for direct detection of MRSA/MRSCoN in blood cultures and in abscess punctures based on mecA and species specific identification of S. aureus by polymerase-chain reaction (PCR). Materials and methods: We examined 77 growth-positive BACTEC blood cultures and 50 abscess punctures by routine microbiological assay and simultaneous PCR detection of MRSA/MRSCoN. The speci&#64257;city of the PCR was evaluated by using DNA from another 15 microbial species for negative controls. We determined the minimum inhibitory concentration (MIC) of oxacillin, vancomycin, tigecycline, linezolid, levofloxacin, clindamycin, and erythromycin against the S. aureus isolates using the E-test.&nbsp; Results: In the blood cultures, the two methods detected 39.3% of MRSA, and 93.9% of MRCoNS. In the punctures, the PCR assay identified 20.9% of MRSA and 79.2% of MSSA. In the puncture cases, there were three PCR MRSA positive and culture negative samples. Screening for susceptibility to 14 antimicrobial agents demonstrated significantly higher (p<0.05) methicillin resistance in blood culture isolates than in the puncture ones (39.3% and 20.0%, respectively).&nbsp; Conclusion: The new PCR protocol was very fast and specific. It was more sensitive in detecting MRSA from abscess punctures than the routine microbiological techniques. This protocol will speed up the right choice of empirical therapy, which is extremely important for saving patients&rsquo; lives.

Influence of Selective Bowel Decontamination on the Organisms Recovered During Bacteremia in Neutropenic Patients

2004 ◽  
Vol 25 (8) ◽  
pp. 685-689 ◽  
Author(s):  
Florian Daxboeck ◽  
Werner Rabitsch ◽  
Alexander Blacky ◽  
Maria Stadler ◽  
Paul A. Kyrle ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Staphylococcus Aureus ◽  
Bone Marrow ◽  
Anaerobic Bacteria ◽  
Blood Cultures ◽  
University Teaching ◽  
Coagulase Negative Staphylococci ◽  
Gram Negative ◽  
Neutropenic Patients ◽  
Care Ward

AbstractObjective:To assess the influence of prophylactic selective bowel decontamination (SBD) on the spectrum of microbes causing bloodstream infection (BSI).Design:The microbes causing BSI in neutropenic patients of a hematologic ward (HW) and a bone marrow transplantation unit (BMTU), respectively, were compared by retrospective analysis of blood culture results from January 1996 to June 2003.Setting:A 30-bed HW (no SBD) and a BMTU including a 7-bed normal care ward and an 8-bed intensive care unit (SBD used) of a 2,200-bed university teaching hospital.Results:The overall incidences of bacteremia in the HW and the BMTU were similar (72.6 vs 70.6 episodes per 1,000 admissions; P = .8). Two hundred twenty episodes of BSI were recorded in 164 neutropenic patients of the HW and 153 episodes in 127 neutropenic patients of the BMTU. Enterobacteriaceae (OR, 3.14; CI95, 1.67–5.97; P = .0002) and Streptococcus species (OR, 2.04; CI95, 1.14–3.70; P = .015) were observed more frequently in HW patients and coagulase-negative staphylococci more frequently in BMTU patients (OR, 0.15; CI95, 0.09–0.26; P< .00001). No statistically significant differences were found for gram-negative nonfermentative bacilli (P = .53), Staphylococcus aureus (P = .21), Enterococcus species (P = .48), anaerobic bacteria (P = .1), or fungi (P = .50).Conclusions:SBD did not lead to a significant reduction in the incidence of bacteremia, but significant changes in microbes recovered from blood cultures were observed. SBD should be considered when empiric antimicrobial therapy is prescribed for suspected BSI.

Comparison of conventional and rapid methods for the detection of methicillin resistance in Staphylococcus aureus from blood cultures

2017 ◽  
Vol 20 (2) ◽  
pp. 196
Author(s):  
Ashwani Kumar ◽  
Bineeta Kashyap ◽  
Anurag Mehndiratta
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Blood Cultures ◽  
Rapid Methods

Increased Antimicrobial Resistance of Pathogenic Coagulase-Negative Staphylococci From Blood Cultures (1961–1981)

1984 ◽  
Vol 5 (3) ◽  
pp. 142-143 ◽  
Author(s):  
D.J. Flournoy
Keyword(s):  
Staphylococcus Aureus ◽  
Blood Culture ◽  
Prosthetic Heart Valve ◽  
Bacterial Endocarditis ◽  
Blood Cultures ◽  
Coagulase Negative Staphylococci ◽  
Difficult Decision ◽  
Antimicrobial Susceptibilities ◽  
Cons Isolate ◽  
Intravenous Catheters

Coagulase-negative staphylococci (CONS) have only recently gained notoriety as pathogens. Several reports have established their pathogenicity in bacterial endocarditis, prosthetic heart valve endocarditis, intraventricular shunts for treatment of hydrocephalus and intravenous catheters. One difficult decision for physicians is determining whether a particular CONS isolate is pathogenic or contaminant. The differentiation of pathogenic and contaminant CONS has recently been noted, but further studies are needed to aid in this differentiation. Data on antimicrobial susceptibilities of positive blood culture isolates were recently compiled at this institution. This report compares antimicrobial susceptibilities of pathogenic and contaminant CONS and Staphylococcus aureus blood culture isolates from 1961-1981 at this institution.

scholarly journals Novel Type XII Staphylococcal Cassette ChromosomemecHarboring a New Cassette Chromosome Recombinase, CcrC2

2015 ◽  
Vol 59 (12) ◽  
pp. 7597-7601 ◽  
Author(s):  
Zhaowei Wu ◽  
Fan Li ◽  
Dongliang Liu ◽  
Huping Xue ◽  
Xin Zhao
Keyword(s):  
United States ◽  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
The United States ◽  
Gene Complex ◽  
Coagulase Negative Staphylococci ◽  
Typing Method ◽  
Content Type ◽  
Staphylococcal Cassette Chromosome ◽  
Sequence Identities

ABSTRACTExcision and integration of staphylococcal cassette chromosomemec(SCCmec) are mediated by cassette chromosome recombinases (Ccr), which play a crucial role in the worldwide spread of methicillin resistance in staphylococci. We report a novelccrgene,ccrC2, in the SCCmecof aStaphylococcus aureusisolate, BA01611, which showed 62.6% to 69.4% sequence identities to all publishedccrC1sequences. A further survey found that theccrC2gene was mainly located among coagulase-negative staphylococci (CoNS) and could be found in staphylococcal isolates from China, the United States, France, and Germany. Theccrgene complex harboring theccrC2gene was designated a type 9 complex, and the SCCmecof BA01611 was considered a novel type and was designated type XII (9C2). This novel SCCmecelement in BA01611 was flanked by a pseudo-SCC element (ΨSCCBA01611) carrying a truncatedccrA1gene. Both individual SCC elements and a composite SCC were excised from the chromosome based on detection of extrachromosomal circular intermediates. We advocate inclusion of the ccrC2gene and type 9ccrgene complex during revision of the SCCmectyping method.

scholarly journals Molecular detection and typing of methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative staphylococci isolated from cattle, animal handlers, and their environment from Karnataka, Southern Province of India

2019 ◽  
Vol 12 (11) ◽  
pp. 1760-1768 ◽  
Author(s):  
Nimita Venugopal ◽  
Susweta Mitra ◽  
Rituparna Tewari ◽  
Feroze Ganaie ◽  
Rajeswari Shome ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Animal Health ◽  
Sccmec Type ◽  
Meca Gene ◽  
Molecular Characteristics ◽  
Rrna Gene ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant

Background and Aim: Methicillin-resistant staphylococci are among the emerging pathogens which have become a threat to both human and animal health. The present investigation intended to examine the occurrence and the molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative staphylococci (MRCoNS) recovered from cattle, its handlers, and their environment. Materials and Methods: A total of 666 specimens were subjected to culture method and genus-specific polymerase chain reaction (PCR) for the identification of Staphylococcus. Methicillin resistance was substantiated by PCR identification of mecA and mecC resistance determinants. Species-specific identification of mecA positive isolates was conducted by multiplex PCR. The unidentified species were deciphered by 16S rRNA gene sequencing approach. The mecA positive isolates were further characterized by staphylococcal cassette chromosome mec (SCCmec) typing and multilocus sequence typing (MLST). Results: Duplex PCR identified 728 Staphylococcus isolates, of which 66 (9%) were positive for mecA gene. MRSA constituted 24% of the total mecA positive isolates. Among MRCoNS, Staphylococcus epidermidis (42%), and Staphylococcus haemolyticus (11%) were the most common species identified. Overall, 47% of the mecA positive isolates belonged to SCCmec type V. MLST analysis showed eight different sequence types (STs) among MRSA isolates of which five were novel STs. Among methicillin-resistant S. epidermidis, 19 different STs were found, of which nine novel STs were detected. Conclusion: The increase in the prevalence of mecA positive staphylococci, especially MRCoNS in cattle is a great concern in view of their transmission potential. Hence, continuous monitoring and molecular characterization of methicillin-resistant staphylococci should be elucidated in human and animal sectors so as to prevent the spread of these resistant pathogens.

scholarly journals Comparison of culture media for detecting methicillin resistance in Staphylococcus aureus and coagulase negative staphylococci.

1987 ◽  
Vol 40 (10) ◽  
pp. 1178-1181 ◽  
Author(s):  
L M Milne ◽  
G D Curtis ◽  
M Crow ◽  
W A Kraak ◽  
J B Selkon
Keyword(s):  
Staphylococcus Aureus ◽  
Culture Media ◽  
Methicillin Resistance ◽  
Coagulase Negative Staphylococci
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