scholarly journals Application of Immobilized Pointed Gourd (Trichosanthes dioica) Peroxidase-Concanavalin A Complex on Calcium Alginate Pectin Gel in Decolorization of Synthetic Dyes Using Batch Processes and Continuous Two Reactor System

Author(s):  
Farrukh Jamal
2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Gulnur Arabaci ◽  
Ayse Usluoglu

Water pollution due to release of industrial wastewater has already become a serious problem in almost every industry using dyes to color its products. In this work, polyphenol oxidase enzyme from quince (Cydonia Oblonga) leaves immobilized on calcium alginate beads was used for the successful and effective decolorization of textile industrial effluent. Polyphenol oxidase (PPO) enzyme was extracted from quince (Cydonia Oblonga) leaves and immobilized on calcium alginate beads. The kinetic properties of free and immobilized PPO were determined. Quince leaf PPO enzyme stability was increased after immobilization. The immobilized and free enzymes were employed for the decolorization of textile dyes. The dye solutions were prepared in the concentration of 100 mg/L in distilled water and incubated with free and immobilized quince (Cydonia Oblonga) leaf PPO for one hour. The percent decolorization was calculated by taking untreated dye solution. Immobilized PPO was significantly more effective in decolorizing the dyes as compared to free enzyme. Our results showed that the immobilized quince leaf PPO enzyme could be efficiently used for the removal of synthetic dyes from industrial effluents.


Author(s):  
K. Subramanya Sastry ◽  
Bikash Mandal ◽  
John Hammond ◽  
S. W. Scott ◽  
R. W. Briddon

1970 ◽  
Vol 35 (1) ◽  
pp. 135-142 ◽  
Author(s):  
MA Malek ◽  
D Khanam ◽  
M Khatun ◽  
MH Molla ◽  
MA Mannan

An experiment was conducted to study the in vitro culture of pointed gourd. Cotyledon rescued from physiologically matured seeds (PMS) and immatured seeds (IMS) of pointed gourd were used as explants. Cotyledon excised from PMS responded very well in all culture conditions. Plant regenerated from cotyledon of PMS ranged from 38 to 96% in different hormonal formulations of culture media. Highest percentage of shoot regeneration was observed in MS + 1.0 mg/l BAP and lowest in MS + 2.5 mg/l BAP. No plant regeneration was observed in cotyledon from immatured seeds. The highest percentage of root induction (99%) was achieved in half MS medium supplemented with 0.5 mg/l NAA. The regenerated plantlets were successfully established in earthen pot. Keywords: Cotyledon; in vitro; pointed gourd. DOI: 10.3329/bjar.v35i1.5874Bangladesh J. Agril. Res. 35(1) : 135-142, March 2010


2007 ◽  
Vol 20 (1) ◽  
pp. 47-52
Author(s):  
M. A. Malek ◽  
M. A. Bari Miah ◽  
M. O. Islam ◽  
A. M. M. Mosiul Hoque ◽  
R. Gomes

Genetic variability, heritability in broad sense and expected genetic advance as percent of mean for fruit yield and nine characters were studied in 25 pointed gourd genotypes. Fruits per plant and fruit yield per plant showed maximum variability. Genotypic coefficient of variation was found high for fruit yield and number of fruits per plant. All the characters under study were highly heritable in nature. High heritability coupled with high genetic advance was observed for fruit yield and fruits per plant, which indicated that these characters are more reliable for effective selection.DOI: http://dx.doi.org/10.3329/bjpbg.v20i1.17026


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