In vitro culture of pointed gourd (Trichosanthes dioica Roxb.)

1970 ◽  
Vol 35 (1) ◽  
pp. 135-142 ◽  
Author(s):  
MA Malek ◽  
D Khanam ◽  
M Khatun ◽  
MH Molla ◽  
MA Mannan
Keyword(s):  
Plant Regeneration ◽  
In Vitro Culture ◽  
Culture Media ◽  
Culture Conditions ◽  
Root Induction ◽  
Ms Medium ◽  
Trichosanthes Dioica ◽  
Pointed Gourd ◽  
Regenerated Plantlets

An experiment was conducted to study the in vitro culture of pointed gourd. Cotyledon rescued from physiologically matured seeds (PMS) and immatured seeds (IMS) of pointed gourd were used as explants. Cotyledon excised from PMS responded very well in all culture conditions. Plant regenerated from cotyledon of PMS ranged from 38 to 96% in different hormonal formulations of culture media. Highest percentage of shoot regeneration was observed in MS + 1.0 mg/l BAP and lowest in MS + 2.5 mg/l BAP. No plant regeneration was observed in cotyledon from immatured seeds. The highest percentage of root induction (99%) was achieved in half MS medium supplemented with 0.5 mg/l NAA. The regenerated plantlets were successfully established in earthen pot. Keywords: Cotyledon; in vitro; pointed gourd. DOI: 10.3329/bjar.v35i1.5874Bangladesh J. Agril. Res. 35(1) : 135-142, March 2010

scholarly journals In vitro regeneration through callus in pointed gourd (Trichosanthes dioica Roxb.)

1970 ◽  
Vol 35 (3) ◽  
pp. 465-473 ◽  
Author(s):  
MA Malek ◽  
MA Mannan ◽  
D Khanam ◽  
MH Molla ◽  
M Khatun
Keyword(s):  
Plant Regeneration ◽  
In Vitro Regeneration ◽  
Root Induction ◽  
Ms Medium ◽  
In Vitro Plant Regeneration ◽  
Best Response ◽  
Pointed Gourd ◽  
Regenerated Plantlets ◽  
Regeneration Study

An efficient protocol was developed for in vitro plant regeneration and multiplication through callus culture in pointed gourd. Among the explants, highest percentage of cotyledon explants (92.00%) produced callus when this explant cultured in MS medium supplemented with NAA (0.1, 0.5, 1.0, 1.5, and 2.0 mg/l) and 2, 4-D (0.1, 0.5, 1.0, 1.5, and 2.0 mg/l). The highest number of shoots per explant was observed in MS + 0.5 mg/l BAP + 0.5 mg/l NAA followed by 1.0 mg/l BAP + 0.5 mg/l NAA when inter-node derived callus cultured in MS medium. Among the explants derived calli from leaf, inter-node and cotyledon in in vitro regeneration study, inter-node appeared as the most suitable explant for callusing and plant regeneration. The best response towards root induction was achieved on half MS medium supplemented with 0.5 mg/l NAA. The regenerated plantlets were successfully established in prepared earthen soil pot. Keywords: In vitro regeneration; pointed gourd. DOI: 10.3329/bjar.v35i3.6453Bangladesh J. Agril. Res. 35(3) : 465-473

scholarly journals In vitro regeneration in pointed gourd

2008 ◽  
Vol 32 (3) ◽  
pp. 461-471 ◽  
Author(s):  
MA Malek ◽  
MA Bari Miah ◽  
M AL-Amin ◽  
D Khanam ◽  
M Khatun
Keyword(s):  
Shoot Regeneration ◽  
Nodal Segment ◽  
Root Induction ◽  
Ms Medium ◽  
Shoot Induction ◽  
Best Response ◽  
Pointed Gourd ◽  
Regenerated Plantlets ◽  
Better Than

An efficient protocol was developed for plant regeneration, multiplication and rooting under in vitro condition in pointed gourd. Highest percent of shoot regeneration was 93.86 when nodal explants were cultured on MS+2.0 mg/1 BAP. The maximum number of shoots (4.00) per explant was observed in MS + 2.0 mg/1 BAP + 0.3 mg/1 NAA from nodal segment. Among the two explants, nodal segment was found better for shoot regeneration. Female genotypes responded better than the male genotypes for shoot induction and proliferation. Lower nodal segment performed the best shoot regeneration. The best response towards root induction was achieved on half MS medium supplemented with 0.5 mg/1 NAA. The regenerated plantlets were successfully established in prepared earthen soil pot.DOI: http://dx.doi.org/10.3329/bjar.v32i3.548Bangladesh J. Agril. Res. 32(3) : 461-471, September 2007

scholarly journals In vitro micropropagation of pointed gourd (Trichosanthes dioica Roxb.) from shoot tip and nodal segment

2012 ◽  
Vol 47 (2) ◽  
pp. 217-222 ◽  
Author(s):  
Amdadul Huq ◽  
Shahina Akter ◽  
Shahina Islam ◽  
Salim Khan
Keyword(s):  
Shoot Regeneration ◽  
Garden Soil ◽  
Nodal Segment ◽  
Root Induction ◽  
Ms Medium ◽  
Best Response ◽  
In Vitro Micropropagation ◽  
Trichosanthes Dioica ◽  
Pointed Gourd

An efficient protocol was developed for plant regeneration, multiplication and rooting under in vitro condition in pointed gourd. Highest percent of shoot regeneration was 91.66%, when nodal explants were cultured on MS+1.0 mg/1 BAP. The maximum number of shoots (4.8) per explant was observed in MS + 1.0 mg/1 BAP + 0.2 mg/1 NAA from nodal segment. Among the two explants, nodal segment was found better for shoot regeneration and multiplication. The best response towards root induction was achieved on half MS medium supplemented with 0.4 mg/1 NAA. The regenerated healthy rooted plantlets were transferred to small plastic pot containing garden soil and compost in a ratio of 2:1. Immediately after transplantation the plantlets were covered with polythene bag to prevent desiccation. After acclimatization the plantlets were successfully transferred to the garden soil and the percentage of survivability in such condition was 90%.   DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11457   Bangladesh J. Sci. Ind. Res. 47(2), 217-222, 2012  

scholarly journals Cell suspension culture and plant regeneration of a Brazilian plantain, cultivar Terra

2008 ◽  
Vol 43 (10) ◽  
pp. 1325-1330 ◽  
Author(s):  
Lucymeire Souza Morais-Lino ◽  
Janay Almeida dos Santos-Serejo ◽  
Sebastião de Oliveira e Silva ◽  
José Raniere Ferreira de Santana ◽  
Adilson Kenji Kobayashi
Keyword(s):  
Plant Regeneration ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Somatic Embryos ◽  
Culture Media ◽  
Ms Medium ◽  
Regenerated Plants ◽  
Male Flowers

The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no visible somaclonal variation was observed in vitro. It is possible to regenerate plants from cell suspensions of plantain banana cultivar Terra using MS medium supplemented with 11.4 µM of IAA and 2.2 µM of BAP.

scholarly journals Haploid plantlet regeneration through anther culture in oilseed Brassica species

1970 ◽  
Vol 34 (4) ◽  
pp. 693-703 ◽  
Author(s):  
MA Alam ◽  
MA Haque ◽  
MR Hossain ◽  
SC Sarker ◽  
R Afroz
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Growth Regulators ◽  
Callus Induction ◽  
Brassica Species ◽  
Plantlet Regeneration ◽  
Root Induction ◽  
Ms Medium ◽  
Half Strength

Anther of five varieties of Brassica species, namely BARI Shariaha-7, Tori-7, Agrani, Daulat and Safal were cultured in vitro to observe their regeneration potentiality. Different concentrations and combinations of growth regulators were supplemented in MS medium. The range of callus induction was 12.50-87.50 %. Maximum callus induction (75.00%) was observed on MS +4 mg/L 2, 4-D + 1.0 mg/L BAP. Among the genotypes, BARI Sharisha-7 showed the highest percentage of callus induction (60.42%). Among the treatments, highest percentage of shoot regeneration (75.00%) was observed on MS + 4 mg/L BAP + 1.0 mg/L NAA. BARI Sharisha-7 also showed the highest rate of plant regeneration (66.67%). Root induction was highest (75%) on half strength MS medium supplemented with 1.0 mg/L IBA and 0.5 mg/L NAA. The plantlets with sufficient roots thus obtained were transferred successfully to plastic pots and subsequently to the field. BARI Sharisha-7 and Tori-7 survived easily in the pots as well as in the field but Safal was very poor in survivability both in the pots and in the field. Key Words: Brassica; haploid; anther culture; in vitro regeneration.DOI: 10.3329/bjar.v34i4.5844Bangladesh J. Agril. Res. 34(4) : 693-703, December 2009 

scholarly journals In Vitro Culture of Heuchera villosa ‘Caramel’

HortScience ◽  
2017 ◽  
Vol 52 (4) ◽  
pp. 622-624 ◽  
Author(s):  
Hua Q. Zhao ◽  
Qing H. He ◽  
Li L. Song ◽  
Mei F. Hou ◽  
Zhi G. Zhang
Keyword(s):  
Acetic Acid ◽  
In Vitro Culture ◽  
Butyric Acid ◽  
Shoot Regeneration ◽  
Ms Medium ◽  
Shoot Induction ◽  
Induction Rate ◽  
Regenerated Plantlets ◽  
Indole 3 Acetic Acid

The procedure for Heuchera villosa ‘Caramel’ propagation was investigated, which involves shoot regeneration, rooting of regenerated shoots, and acclimation of regenerated plantlets. Petioles, as explants, were cultured on MS medium supplemented with 1-naphthylacetic acid (NAA), benzylaminopurine (BA), thidiazuron (TDZ) and callus formed on all media. Shoots were observed to proliferate from callus on media with BA and NAA, whereas no shoots regenerated on media with TDZ and NAA. On media containing 0.5 or 1.0 mg·L−1 BA in combination with NAA, the regenerated shoots showed severe hyperhydricity, whereas on media containing 0.1 mg·L−1 BA in combination with NAA, the regenerated shoots grew normally. The highest shoot induction rate, 90.6%, was obtained on media containing 0.1 mg·L−1 BA and 0.01 mg·L−1 NAA. The effects of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), and NAA on rooting of H. villosa ‘Caramel’ was explored. The highest rooting rate (95%) was obtained on 1/2 MS medium containing 0.2 mg·L−1 NAA. In the subsequent acclimation experiments, about 85% of rooted plantlets survived and grew normally.

scholarly journals Efisiensi Mikropropagasi Pisang Kepok Amorang melalui Modifikasi Formula Media dan Temperatur

2016 ◽  
Vol 6 (2) ◽  
pp. 91
Author(s):  
Yati Supriati
Keyword(s):  
In Vitro Culture ◽  
Incubation Temperature ◽  
Culture Media ◽  
Shoot Multiplication ◽  
Root Induction ◽  
Incubation Condition ◽  
Growth Environment ◽  
Media Formulation ◽  
The Media

<p>Micropropagation Efficiency of Banana cv Kepok<br />Amorang through Modifications of Culture Media and<br />Incubation Temperature. Yati Supriati. The budless<br />banana cv Kepok Amorang is potentially commercialized<br />due to its sweet taste and does not have flower bud, hence<br />reduced the potential of being infected by the blood disease<br />pathogen. Enhancement of banana industry needs continuous<br />supplies of large number banana seedlings. In vitro<br />culture enable the production of seedlings in a large scale,<br />uniform, quick. The research aims: (1) to formulate an<br />efficient medium for in vitro multiplication of cv Kepok<br />Amorang shoot, (2) to identify efficient growth environment<br />for in vitro culture of cv Kepok Amorang, and (3) to formulate<br />an efficient culture medium for roots inductions of cv<br />Kepok Amorang. The plant material used was in vitro culture<br />of Kepok cv Amorang, 2 cm in height without leaf and root.<br />The media formulation for shoot multiplication were full<br />strength, half strength, one fourth strength MS media,<br />supplemented with either 1, 3, or 5 ppm IBA. On optimization<br />step, the media tested were MS, Knop, Knop and<br />Heller, Hyponex N, Growmore N, and Rosasol N containing<br />of 1 ppm BA. The explants were incubated in culture room<br />with 8, 12, and 16 hours photoperiod with temperatures 30oC<br />(non air conditioned) and 25oC (air conditioned). The root<br />induction trial was done using MS, Knop, Knop and Heller,<br />Hyponex N, Growmore N, and Rosasol N media containing<br />of 1 ppm and 3 ppm IBA. The results showed that the best<br />medium formula for shoot multiplication was ¼ MS + 1 ppm<br />IBA. The best incubation condition was 16 hours photoperiods<br />at 30oC. The best media for root induction was<br />Hyponex 2 g/l + 1 ppm IBA. This culture method reduced<br />cost by Rp 261.7 per plantlet through efficiency of media<br />formulation and electricity use.</p>

scholarly journals In Vitro Regeneration Of Brinjal (Solanum Melongena L.)

1970 ◽  
Vol 36 (3) ◽  
pp. 397-406 ◽  
Author(s):  
BP Ray ◽  
L Hassan ◽  
KM Nasiruddin
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Callus Formation ◽  
Solanum Melongena ◽  
Ms Medium ◽  
Fresh Weight ◽  
Normal Environment ◽  
Regenerated Plantlets

The effect of different explants and concentrations of BAP and NAA on induction of callus and plant regeneration of brinjal cv. Jhumki were investigated. The treatment combinations were BAP (0. 2.0. 3.0, and 4.0 mg/l) and NAA (0. 0.1, 0.5, and 1.0 mg/l). The rate of callus formation varied in different treatments. The highest amount of callus (48.66%) was produced on MS medium containing 2.0 mg/l BAP and 0.5 mg/l NAA from stem, and 8.2 days required for callus induction. The highest fresh weight of callus was 1.12g from stem and 0.48g from root. The number of shoot regenerated through callus from stem containing 2.0 mg/l BAP and 0.5 mg/l NAA was 3.4 (23.287%) and days required for 38.8 days. All regenerated plantlets survived in normal environment. Keywords: NAA; BAP; regeneration; brinjal. DOI: http://dx.doi.org/10.3329/bjar.v36i3.9268 BJAR 2011; 36(3): 397-406

scholarly journals In vitro regeneration potentiality of Brassica genotypes in differential growth regulators

1970 ◽  
Vol 35 (2) ◽  
pp. 189-199 ◽  
Author(s):  
MMA Khan ◽  
ABM Arif Hasan Khan Robin ◽  
MAN Nazim-Ud-Dowla ◽  
SK Talukder ◽  
L Hassan
Keyword(s):  
Plant Regeneration ◽  
Growth Regulators ◽  
Callus Induction ◽  
Root Formation ◽  
Ms Medium ◽  
Differential Growth ◽  
The Media ◽  
Regenerated Plantlets

Petiole of six genotypes of oilseed Brassica viz. Tori-7, Sampad, Kallyania, BARI Sarisha-7, BARI Sarisha-8, and MM 20-3 were cultured in MS medium with different concentrations of BAP, NAA, and AgNO3 for callus induction and subsequent plant regeneration. The highest percentage of callus induction (91.43%) was observed in Tori-7 in the media supplemented with 2 mg/L BAP, 0.1 mg/L NAA and 2.0 mg/L AgNO3. Calli were maintained in order to get sufficient number of regenerants. With the increased concentration of BAP, the highest percentage (57.14) of regenerants were found in Tori-7 followed by Sampad (33.13%) and BARI Sarisha-8 (31.42%) in MS media supplemented with 2.5 mg/L BAP, 0.1 mg/L NAA and 2.0 mg/L AgNO3. Root formation from the regenerants was found best in half MS medium supplemented with 0.5 mg/L NAA in genotype Tori-7. Regenerated plantlets of four genotypes (Tori-7, BARI Sarisha-8, Kallyania, BARI Sarisha-7) were successfully established in the field.Keywords: AgNO3; BAP; Brassica; NAA; regeneration.  DOI: 10.3329/bjar.v35i2.5881Bangladesh J. Agril. Res. 35(2) : 189-199, June 2010

scholarly journals Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

2017 ◽  
Vol 5 (2) ◽  
pp. 15-26 ◽  
Author(s):  
Raihan I Raju ◽  
Shyamal K Roy
Keyword(s):  
In Vitro Culture ◽  
Basal Medium ◽  
Nodal Segments ◽  
Garden Soil ◽  
Root Induction ◽  
Ms Medium ◽  
Mass Propagation ◽  
Surface Sterilization ◽  
Half Strength

Protocol for mass propagation of Bambusa bamboos (L.) Voss was developed through in vitro culture. Nodal segments containing pre-existing axillary bud, after surface sterilization, were inoculated on liquid Murashige and Skoog’s (MS) basal medium containing different concentrations and combinations of cytokinins (BAP, TDZ and Kn). The highest direct shoot induction (90%) was obtained in the MS liquid medium supplemented with 2.0 mg/l BAP and 1.0 mg/l TDZ with maximum average number of shoots (3.14 ± 0.06) per explant. Highest shoot multiplication (16.58 ± 0.24 shoots per culture) with highest average shoot length (9.21 ± 0.13 cm) was obtained when in vitro raised shoots were cultured in gelrite gelled MS medium in conjunction with 2.0 mg/l BAP and 1.0 mg/l TDZ. Incorporation of 10% coconut water with 4% sucrose in the above mentioned medium resulted satisfactory shoot growth and development with an average 26.7 ± 0.60 shoots per culture. For root induction, in vitro raised shoots were divided into clumps of 4-5 shoots in each clump and transferred onto both liquid and gelled half-strength MS medium containing different concentrations and combinations of auxins (IBA and NAA). Maximum rooting (86.67%) was achieved in half-strength of MS medium fortified with 2.5 mg/l IBA and 2.5 mg/l NAA with an average 8.72 ± 0.42 root per shoot. The rooted plantlets were then transferred to polybags containing garden soil, sand and compost mixture with 1:1:1 ratio. After a month the hardened plantlets were then transferred to the larger pots containing garden soil and compost with 1:1 ratio for sufficient growth and finally transplanted to the field. In this process, the highest 100% survivability was recorded from well-established rooted plantlets. The regenerated plants showed well developed root and shoot systems in field condition.Jahangirnagar University J. Biol. Sci. 5(2): 15-26, 2016 (December)

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