Estrogenic Effect of Bambusa arundinacea, Trichosanthes dioica and Punica granatum on Rats

Background: Phytoestrogens have recently become a hot topic among scientists. Phytoestrogens’ estrogen-like properties have led to their widespread use in the reproductive system. The aim of this research was to see whether the ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum had any estrogenic activity in female wistar rats. Methods: In female wistar rats, the estrogenic effect was studied using a uterotropic assay, vaginal cytology and vaginal opening. In ovariectomized immature and mature female wistar rats, a 400 mg/kg body weight (b.w.) dose of ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum was given. Result: When compared to ovariectomized control rats, the uterine wet weight increased significantly. The estrogen-treated rats had only cornified epithelial cells, indicating the existence of oestrogen, as well as 100% vaginal opening. At 400 mg/kg b.w., the ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum demonstrated promising estrogenic activity, as evidenced by uterotropic assays, vaginal opening measurements and histopathological changes. As a result of this research, it’s possible to infer that the ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum play an important role in estrogenic activity in female rats.

A A REVIEW: MANAGEMENT OF RASA PRADOSHAJA VIKARA WITH TRICHOSANTHES DIOICA (PATOL).

In Ayurvedic Samhitas daily regimen are mentioned such as Dinacharya, Ritucharya, Ahara, and Vihara. Now days due to stressful lifestyle intake of junk food, sedentary life, excessive workload reduces individual happiness and predispose to many diseases. While describing Rasa Rakta dushti hetu, Acharyas have mentioned hetus like guru, snigdha, vidahi, ushna ahar sevana. Due to this there is favourable condition for development of Rasa pradoshaj vikara such as Jwara, Pandutva, Shrotorodha etc. Day by day such Rasa pradoshaj vikara get increased due to consumption of above hetus. All these hetus develop toxins inside the body known as Ama. This Ama enters in the Rasa dhatu and produces various diseases. While treating Rasa pradoshaj vikara langhana and raktapittahari kriya should be done respectively. Tikta rasa is predominantly used for Ama pachan and Kapha Pitta shaman. Since ancient times the fruits and leaves of Patola are widely consumed as a vegetable and different parts of this plant are used in traditional medicine to treat Rasa Rakta pradoshaj vikara. Patola has tikta rasa and with the help of this it acts on Kapha Pitta dosha and Rasa Rakta datu. According to Acharya Charaka and Vagbhata Patola is mentioned in Rasapachaka kashaya in Ayurvedic text. It has antipyretic, laxative, cardiotonic, antiulcer, antihelmentic property. Patola has tikta rasa and with the help of this it acts on Kapha Pitta dosha and Rasa Rakta datu. This paper elaborates the utility of Patola in management of Rasa pradoshaja vikara.

Novel Ribosome-inactivating Protein (RIP) Isolated from Trichosanthes dioica Induces Apoptosis in HeLa Cell Line

Ribosome-inactivating proteins (RIPs) are toxic N-glycosidases that depurinate eukaryotic and prokaryotic rRNAs and thus interrupt protein synthesis during translation. In the present study, a protein of around 32 kDa, supposedly a RIP isolated from Trichosanthes dioica, was assessed for its potential to induce apoptosis in HeLa cells. Cell viability assay was done to measure cell proliferation and survivability. It was observed that cells viability decreased with the increase of decrease in dilution, i.e. when the sample was an undiluted one, the viability decreased drastically and almost came to less than 10%. To further check whether the isolated RIP could induce apoptosis, HeLa cells were treated with the test RIP. Immunoblotting was carried out using PARP poly (ADP-ribose) polymerase (PARP-1), a 113 kDa nuclear enzyme, which is considered a hallmark of cells undergoing apoptosis. HeLa cells were further analyzed for loss of mitochondrial membrane potential with JC-1 dye, which is an early event during apoptosis. Increased PARP breakdown in the RIP treated cells indicates that cells undergoing apoptosis and progressive loss of red J-aggregate fluorescence indicate that the isolated RIP from Trichosanthes dioica induces apoptosis in HeLa cells. The ability of apoptosis induction is comparable to another known RIP from Momordica charantia, which was used as a positive control. Promising results from the present study warrants the isolated RIP to be further explored for anticancer activities.