scholarly journals Isolation and characterization of mesenchymal stem cells in orthopaedics and the emergence of compact bone mesenchymal stem cells as a promising surgical adjunct

2020 ◽  
Vol 12 (11) ◽  
pp. 1341-1353
Author(s):  
Albert Anastasio ◽  
Marina Gergues ◽  
Michael S Lebhar ◽  
Pranela Rameshwar ◽  
Joseph Fernandez-Moure
2020 ◽  
Author(s):  
Elsa González‐Cubero ◽  
María Luisa González‐Fernández ◽  
Laura Gutiérrez‐Velasco ◽  
Eliezer Navarro‐Ramírez ◽  
Vega Villar‐Suárez

2021 ◽  
pp. 102341
Author(s):  
Rina Otsuka-Yamaguchi ◽  
Masaaki Kitada ◽  
Yasumasa Kuroda ◽  
Yoshihiro Kushida ◽  
Shohei Wakao ◽  
...  

2019 ◽  
Vol 37 (6) ◽  
pp. 1419-1428
Author(s):  
Qian Liu ◽  
Yaxi Zhu ◽  
Jun Qi ◽  
Peter C. Amadio ◽  
Steven L. Moran ◽  
...  

2019 ◽  
Vol 22 (2) ◽  
pp. 136-143
Author(s):  
Alaa Eldin Abdelhamid ◽  
Mohamed El Shahedy ◽  
Mona Mohammed ◽  
Ghada Aly ◽  
Bassma Dessouki

Author(s):  
Spoorthi Ravi Banavar ◽  
Swati Yeshwant Rawal ◽  
Ian Charles Paterson ◽  
Gurbind Singh ◽  
Fabian Davamani ◽  
...  

2014 ◽  
Vol 240 (2) ◽  
pp. 185-193 ◽  
Author(s):  
Jung-Sun Cho ◽  
Joo-Hoo Park ◽  
Ju-Hyung Kang ◽  
Sung Eun Kim ◽  
Il-Ho Park ◽  
...  

2016 ◽  
Vol 48 (6) ◽  
pp. 653-658 ◽  
Author(s):  
Kuldeep Kumar ◽  
Pranjali Agarwal ◽  
Kinsuk Das ◽  
Bhabesh Mili ◽  
AP Madhusoodan ◽  
...  

2016 ◽  
Vol 2016 ◽  
pp. 1-18 ◽  
Author(s):  
Subhash C. Juneja ◽  
Sowmya Viswanathan ◽  
Milan Ganguly ◽  
Christian Veillette

The procedure for aspiration of bone marrow from the femur of patients undergoing total knee arthroplasty (TKA) or total hip arthroplasty (THA) may vary from an OR (operating room) to OR based on the surgeon’s skill and may lead to varied extent of clotting of the marrow and this, in turn, presents difficulty in the isolation of mesenchymal stem cells (MSCs) from such clotted bone marrow. We present a simple detailed protocol for aspirating bone marrow from such patients, isolation, and characterization of MSCs from the aspirated bone marrow specimens and show that the bone marrow presented no clotting or exhibited minimal clotting. This represents an economical source and convenient source of MSCs from bone marrow for use in regenerative medicine. Also, we presented the detailed protocol and showed that the MSCs derived from such bone marrow specimens exhibited MSCs characteristics and generated micromass cartilages, the recipe for regenerative medicine for osteoarthritis. The protocols we presented can be used as standard operating procedures (SOPs) by researchers and clinicians.


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