In vitro shoot regeneration from leaf explants of Echinops kebericho: an endangered endemic medicinal plant

A shoot regeneration system for Plectranthus neochilus was studied from leaf explants. Leaves developed under in vitro conditions were cultured on Wood Plant Medium supplemented with 0.2mg dm-3 α-naphthaleneacetic acid (NAA) and different 6-benzilaminopurine (BAP) or thidiazuron (TDZ) concentrations (0, 1.5, 3.0, 4.5 and 6.0mg dm-3). An increase in percentage of responsive explants (85.3%) and in the number of shoots developed per explant (3.2) was observed when the explants were treated with 5.3 and 4.7mg dm-3 BAP, respectively. The leaf explants cultured on media supplemented with TDZ became vitreous and did not form buds. The regeneration system used is efficient for boldo bud induction and shoot development, showing high potential for advanced cellular and molecular studies.

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In Vitro Propagation of Digitalis trojana Ivanina., an Endemic Medicinal Plant of Turkey

10.5772/intechopen.94378 ◽

2020 ◽

Author(s):

Nurşen Çördük ◽

Cüneyt Aki

Keyword(s):

Acetic Acid ◽

Medicinal Plant ◽

In Vitro Propagation ◽

Leaf Explants ◽

Naphthalene Acetic Acid ◽

Ms Medium ◽

Northwestern Turkey ◽

Helen Of Troy ◽

Endemic Medicinal Plant

Digitalis trojana Ivanina is a member of the Plantaginaceae family and known by its common name, Helen of Troy foxglove. It is perennial endemic to Çanakkale and Balıkesir, northwestern Turkey. In order to develop an efficient shoot regeneration protocol, the leaf explants of D. trojana were cultured on Murashige and Skoog (MS) medium containing 6-benzyl adenine (0.1, 0.5, 1.0, 3.0, 5.0 mg/L) and α-naphthalene acetic acid (0.1, 0.5, 1.0 mg/L), 3% (w/v) sucrose and 0.8% (w/v) agar. The highest number of regenerated shoots was obtained from leaf explants that were cultured on MS medium with 3.0 mg/L BA+0.1 mg/L NAA. Regenerated shoots were rooted on MS medium without plant growth regulators. Rooted plants (2–3 cm) were separately transferred to pots containing a mixture of peat and perlite (2:1 v/v) and acclimatized successfully in a growth chamber.

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In vitro Shoot Regeneration from Flower and Leaf Explants in Rhododendron

Biologia Plantarum ◽

10.1023/a:1024363210872 ◽

2003 ◽

Vol 46 (3) ◽

pp. 463-465 ◽

Cited By ~ 8

Author(s):

S. Tomsone ◽

D. Gertnere

Keyword(s):

Shoot Regeneration ◽

Leaf Explants ◽

In Vitro Shoot Regeneration

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In vitro shoot regeneration of Mas Cotek (Ficus deltoidea Jack) A valuable Malay Medicinal Plant

Journal of Tropical Resources and Sustainable Science (JTRSS) ◽

10.47253/jtrss.v3i3.536 ◽

2015 ◽

Vol 3 (3) ◽

pp. 46-50

Author(s):

Ilfah Husna Abdullah Ripain ◽

Paritala Vikram ◽

Fatimah Kayat ◽

Dwi Susanto ◽

Mohammed Aurifullah

Keyword(s):

Medicinal Plants ◽

Medicinal Plant ◽

Shoot Regeneration ◽

Clonal Propagation ◽

Ficus Deltoidea ◽

Economic Area ◽

Over Exploitation ◽

Day By Day ◽

In Vitro Shoot Regeneration

Mas Cotek (Ficus deltoidea) is one of the famous Malay medicinal plants and it is categorized as a priority herb under Entry Point Project 1 (EPP1) of National Key Economic Area (NKEA) Agriculture. Different parts of the plant had been used traditionally to treat various ailments. Due to over-exploitation, the population of many medicinal plants in the nature including Mas Cotek is decreasing day by day. This causes a big challenge to the pharmaceutical and herbal industries as they will run out of the source of plant material to support the demand from the consumers. Hence the present study aims to produce a protocol on shoot regeneration of Mas Cotek by using plant tissue culture. Mature nodes of the plant were aseptically cultured onto Murashige and Skoog (MS) media supplemented with different concentrations and combinations of plant growth regulators like 1.0-3.0 mg/l Kinetin, 1.0-3.0 mg/l BAP, 1.0-3.0 mg/l Kinetin+0.5-1.5 mg/l NAA and 1.0-3.0 mg/l BAP+0.5-1.5 mg/l NAA. 3.0 mg/l BAP wasthe best medium for shoot regeneration of Mas Cotek as it can produce more shoots eitherwhen used singly or in combination with NAA respectively. For the fastest shoot regeneration, MS media with 1.0 mg/l BAP + 0.5 mg/l NAA was more favourable since it can produce shoot within 4 weeks. The protocols developed under this study are highly useful for the mass clonal propagation of Mas Cotek as well as to conserve this valuable medicinal plant for future use.

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In Vitro Shoot Regeneration and Polyploid Induction from Leaves of Hypericum Species

HortScience ◽

10.21273/hortsci.44.7.1957 ◽

2009 ◽

Vol 44 (7) ◽

pp. 1957-1961 ◽

Cited By ~ 14

Author(s):

Elisabeth M. Meyer ◽

Darren H. Touchell ◽

Thomas G. Ranney

Keyword(s):

Shoot Regeneration ◽

Chromosome Doubling ◽

In Vitro Regeneration ◽

Leaf Explants ◽

Shoot Induction ◽

Bluish Green ◽

Spontaneous Chromosome ◽

Ornamental Traits ◽

In Vitro Shoot Regeneration

Hypericum L. H2003-004-016 is a complex hybrid among Hypericum frondosum Michx., Hypericum galioides Lam., and Hypericum kalmianum L. and exhibits valuable ornamental characteristics, including compact habit, bluish green foliage, and showy flowers. Inducing polyploidy may further enhance the ornamental traits of this hybrid and provide new opportunities for hybridizing with other naturally occurring polyploid Hypericum sp. In this study, in vitro shoot regeneration and treatment of regenerative callus with the dinitroaniline herbicide oryzalin (3,5-dinitro-N4,N4-dipropylsufanilamide) were investigated as a means of inducing allopolyploidy. First, in vitro regeneration was optimized for callus and shoot induction by culture of leaf explants on medium supplemented with benzylamino purine (BA) or meta-topolin (mT) at 5, 10, or 15 μM in combination with indoleacetic acid (IAA) at 0, 1.25, 2.5, or 5 μM. Both BA and mT treatments successfully induced regenerative callus and shoots. Multiple regression analysis estimated maximum regenerative callus (94%) and shoot induction (18 shoots per explant) in medium supplemented with 5 μM BA and 3.75 μM IAA. In the second part of the study, exposure of regenerative callus to oryzalin at 0, 7.5, 15, 30, 60, or 90 μM for durations of 3, 6, or 9 d was investigated for polyploid induction. There was no survival for any of the calli in the 60- or 90-μM oryzalin treatments, but calli subjected to the other treatments exhibited some survival and polyploid induction. Duration had no effect on callus survival or ploidy level, but oryzalin concentration was a significant factor in both. The greatest percentage (44%) of polyploids was induced with 30 μM oryzalin. Spontaneous chromosome doubling was observed in 8% of control explants receiving no oryzalin treatment.

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