Clinical application, evaluation and analysis of influencing factors analysis of on tuberculosis ?-Interferon enzyme-linked immunosorbent assay

2021 ◽  
Vol 71 (11) ◽  
pp. 2543-2547
Author(s):  
Ning Xu ◽  
Wenlian Tu ◽  
Taicheng Jin ◽  
Rui Zheng ◽  
Xiaoqin Mao ◽  
...  
Keyword(s):  
Influencing Factors ◽  
Clinical Application ◽  
Interferon Gamma ◽  
Extrapulmonary Tuberculosis ◽  
Final Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sputum Smear ◽  
Interferon Gamma Release Assay ◽  
Specific Cell ◽  
Release Assay

Objective: To assess sensitivity and specificity of the interferon gamma release assay test, and to pinpoint the influencing factors that should be taken care of in clinical application. Methods: The study was conducted at the First People's Hospital in the Yunnan Province of China from October 2018 to March 2019, and comprised samples collected from outpatient and inpatients. To detect mycobacterium tuberculosis, acid-fast staining on sputum smear was performed on relevant tissues suspected of extrapulmonary tuberculosis. Tuberculosis interferon gamma release assay test and pathology samples were examined. Tuberculosis-specific cell reaction assay kit was used for sampling. SysmexXN-2000 haematology analyser, VACUETTE SRS100/II and Beckman Coulter AU5800 were used to perform various analyses. Data was grouped and analysed using R statistical software. Results: Of the 960 samples, 516(53.75%) cases tested positive for tuberculosis infection and 444(46.25%) tested negative. The sensitivity of the pathological results was 86% and the specificity was 96%. The sensitivity of the interferon gamma release assay test was 95% and specificity 82%. Interferon release test, pathological results and final diagnosis showed significant comparisons (p<0.05). Significant relationships were also established for factors, such as age, interferon release quantity, lymphocyte, C-reactive protein and counts of mono-nuclear cell (p<0.05). Conclusions: Interferon gamma release assay test was found to have high consistency with pathological results and final diagnosis and can be used as a subsidiary to traditional clinical imaging and pathological judgment. Key Words: Tuberculosis, Interferon release, CRP, Hormone use. Continuous...  

Diagnostic utility of interferon-gamma release assay in extrapulmonary tuberculosis

2015 ◽  
Vol 82 (1) ◽  
pp. 44-48 ◽  
Author(s):  
Jung Ar Shin ◽  
Yoon Soo Chang ◽  
Hyung Jung Kim ◽  
Chul Min Ahn ◽  
Min Kwang Byun
Keyword(s):  
Interferon Gamma ◽  
Extrapulmonary Tuberculosis ◽  
Interferon Gamma Release Assay ◽  
Diagnostic Utility ◽  
Release Assay

Usefulness of the whole-blood interferon-gamma release assay for diagnosis of extrapulmonary tuberculosis

2009 ◽  
Vol 63 (2) ◽  
pp. 182-187 ◽  
Author(s):  
Kyoung-Ho Song ◽  
Jae Hyun Jeon ◽  
Wan Beom Park ◽  
Sung-Han Kim ◽  
Kyoung Un Park ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Whole Blood ◽  
Extrapulmonary Tuberculosis ◽  
Interferon Gamma Release Assay ◽  
Release Assay

scholarly journals Interferon-Gamma Release Assay Performance in Pulmonary and Extrapulmonary Tuberculosis

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e32652 ◽  
Author(s):  
Yun Feng ◽  
Ni Diao ◽  
Lingyun Shao ◽  
Jing Wu ◽  
Shu Zhang ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Extrapulmonary Tuberculosis ◽  
Interferon Gamma Release Assay ◽  
Assay Performance ◽  
Release Assay

scholarly journals Association of Low Sputum Smear Positivity among Tuberculosis Patients with Interferon-Gamma Release Assay Outcomes of Close Contacts in Japan

2019 ◽  
Vol 16 (19) ◽  
pp. 3713
Author(s):  
Tsuyoshi Ogata ◽  
Natsuki Nagasu ◽  
Ritei Uehara ◽  
Kunihiko Ito
Keyword(s):  
Interferon Gamma ◽  
Acid Fast Bacillus ◽  
Sputum Smear ◽  
Interferon Gamma Release Assay ◽  
Tuberculosis Patients ◽  
Interferon Gamma Release Assays ◽  
Response Measures ◽  
Release Assay ◽  
Smear Negative ◽  
Close Contacts

Risk prediction and response measures may differ in tuberculosis (TB) patients with low sputum smear positivity for acid-fast bacillus (AFB) compared to those who are smear negative. However, previous studies using the tuberculin skin test (TST) did not show that differences in measures are important. This study compared results of interferon-gamma release assays (IGRA) between contacts of pulmonary TB patients with AFB smear positivity and those with smear negativity using QuantiFERON®-TB Gold In-Tube (QFT) assays. Close contacts of TB patients with culture-confirmed infections between April 2010 and December 2012 in Ibaraki, Japan, were enrolled, and 439 Japanese contacts of 129 index TB patients were examined. Adjusted odds ratios of QFT in contacts were 0.68 (95% confidence interval: 0.17–2.8) for AFB scanty patients, 1.12 (0.45–2.8) for AFB 1+, 1.20 (0.48–3.0) for AFB 2+, and 4.96 (1.9–12.9) for AFB 3+, compared to those who were smear negative. Differences in IGRA positivity were not significant between close contacts of TB patients with low positive and negative smears.

scholarly journals Performance of an interferon-gamma release assay for diagnosing latent tuberculosis infection in children

2007 ◽  
Vol 136 (9) ◽  
pp. 1179-1187 ◽  
Author(s):  
K. OKADA ◽  
T. E. MAO ◽  
T. MORI ◽  
T. MIURA ◽  
T. SUGIYAMA ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Latent Tuberculosis ◽  
Mycobacterial Infection ◽  
Sputum Smear ◽  
Interferon Gamma Release Assay ◽  
Test Results ◽  
Positive Rate ◽  
Release Assay ◽  
Latent Tb ◽  
Latent Tb Infection

SUMMARYNewly developed interferon-gamma release assays have become commercially available to detect tuberculosis (TB) infection in adults. However, little is known about their performance in children. We compared test results between the QuantiFERON-TB® Gold test (QFT) and tuberculin skin test (TST) in young children living with pulmonary TB patients in Cambodia. Of 195 children tested with both QFT and TST, the TST-positive rate of 24% was significantly higher than the QFT-positive rate of 17%. The agreement between the test results was considerable (κ-coefficient 0·63). Positive rates increased from 6% to 32% for QFT and from 15% to 43% for TST, according to the sputum smear grades of the index cases. The presence of Bacille Calmette-Guérin (BCG) scars did not significantly affect the results of TST or QFT in a logistic regression analysis. In conclusion, QFT can be a substitute for TST in detecting latent TB infection in childhood contacts aged ⩽5 years, especially in those who may have a false-positive TST due to BCG vaccination or non-tuberculous mycobacterial infection.

scholarly journals Risk of tuberculosis among air passengers estimated by interferon gamma release assay: survey of contact investigations, Japan, 2012 to 2015

2017 ◽  
Vol 22 (12) ◽  
Author(s):  
Masaki Ota ◽  
Seiya Kato
Keyword(s):  
Interferon Gamma ◽  
Air Travel ◽  
World Health ◽  
Skin Tests ◽  
Sputum Smear ◽  
Interferon Gamma Release Assay ◽  
Local Health ◽  
Release Assay ◽  
Contact Investigations ◽  
Health Organization

Although the World Health Organization recommends contact investigations around air travel-associated sputum smear-positive tuberculosis (TB) patients, evidence suggests that the information thus obtained may have overestimated the risk of TB infection because it involved some contacts born in countries with high TB burden who were likely to have been infected with TB in the past, or because tuberculin skin tests were used, which are less specific than the interferon gamma release assay (IGRA) particularly in areas where Bacillus Calmette-Guérin (BCG) vaccination coverage is high. We conducted a questionnaire survey on air travel-associated TB contact investigations in local health offices of Japan from 2012 to 2015, focusing on IGRA positivity. Among 651 air travel-associated TB contacts, average positivity was 3.8% (95% confidence interval (CI): 2.5–5.6) with a statistically significant increasing trend with older age (p < 0.0094). Positivity among 0–34 year-old contacts was 1.0% (95% CI: 0.12–3.5%), suggesting their risk of TB infection is as small as among Japanese young adults with low risk of TB infection (positivity: 0.85–0.90%). Limiting the contact investigation to fewer passengers (within two seats surrounding the index case, rather than two rows) seems reasonable in the case of aircraft with many seats per row.

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