scholarly journals Isolation and Characterization of New Bacillus thuringiensis Strains with Insecticidal Activity to Difficult to Control Lepidopteran Pests

2015 ◽  
Vol 60 (1) ◽  
pp. 103-112
Author(s):  
You Kyoung Lee ◽  
Na Young Jin ◽  
Bo Ram Lee ◽  
Mi Ja Seo ◽  
Young Nam Youn ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Insecticidal Activity ◽  
Isolation And Characterization ◽  
Lepidopteran Pests

scholarly journals Isolation and characterization of a new Bacillus thuringiensis strain with a promising toxicity against Lepidopteran pests

2016 ◽  
Vol 186-187 ◽  
pp. 9-15 ◽  
Author(s):  
Hanen Boukedi ◽  
Sameh Sellami ◽  
Sonia Ktari ◽  
Najeh Belguith-Ben Hassan ◽  
Tahya Sellami-Boudawara ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Isolation And Characterization ◽  
Lepidopteran Pests ◽  
Thuringiensis Strain

Purification and partial amino acid sequence of thuricin S, a new anti-Listeriabacteriocin from Bacillus thuringiensis

2007 ◽  
Vol 53 (2) ◽  
pp. 284-290 ◽  
Author(s):  
Sonia Chehimi ◽  
François Delalande ◽  
Sophie Sablé ◽  
Mohamed-Rabeh Hajlaoui ◽  
Alain Van Dorsselaer ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Proteinase K ◽  
Terminal Sequence ◽  
Partial Amino Acid Sequence ◽  
Ph Values ◽  
Heat Stable ◽  
Isolation And Characterization ◽  
Terminal Amino ◽  
Edman Sequencing

We report the isolation and characterization of a new bacteriocin, thuricin S, produced by the Bacillus thuringiensis subsp. entomocidus HD198 strain. This antibacterial activity is sensitive to proteinase K, is heat-stable, and is stable at a variety of pH values (3–10.5). The monoisotopic mass of thuricin S purified by high perfomance liquid chromatography, as determined with mass spectrometry ESI-TOF-MS, is 3137.61 Da. Edman sequencing and NanoESI-MS/MS experiments provided the sequence of the 18 N-terminal amino acids. Interestingly, thuricin S has the same N-terminal sequence (DWTXWSXL) as bacthuricin F4 and thuricin 17, produced by B. thuringiensis strains BUPM4 and NEB17, respectively, and could therefore be classified as a new subclass IId bacteriocin.

scholarly journals Isolation and characterization of Bacillus thuringiensis strains active against Elasmopalpus lignosellus (Zeller, 1848) (Lepidoptera, Pyralidae)

2017 ◽  
Vol 39 (4) ◽  
pp. 417
Author(s):  
Janaina Zorzetti ◽  
Ana Paula Scaramal Ricietto ◽  
Fernanda Aparecida Pires Fazion ◽  
Ana Maria Meneguim ◽  
Pedro Manuel Oliveira Janeiro Neves ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Isolation And Characterization ◽  
Elasmopalpus Lignosellus ◽  
Lepidoptera Pyralidae

scholarly journals Isolation and characterization of Bacillus thuringiensis (Ernst Berliner) strains indigenous to agricultural soils of Mali

2015 ◽  
Vol 10 (28) ◽  
pp. 2748-2755 ◽  
Author(s):  
Kassogue Adounigna ◽  
Maiga Kadia ◽  
Traore Diakaridia ◽  
Hamadoun Dicko Amadou ◽  
Fane Rokiatou ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Agricultural Soils ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of Bacillus thuringiensis strains from different grain habitats in Turkey

2005 ◽  
Vol 21 (3) ◽  
pp. 285-292 ◽  
Author(s):  
Özgür Apaydin ◽  
A. Fazil Yenidünya ◽  
Şebnem Harsa ◽  
Hatice Güneş
Keyword(s):  
Bacillus Thuringiensis ◽  
Isolation And Characterization

scholarly journals Characterization of Two Novel Bacillus thuringiensis Cry8 Toxins Reveal Differential Specificity of Protoxins or Activated Toxins against Chrysomeloidea Coleopteran Superfamily

Toxins ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 642
Author(s):  
Changlong Shu ◽  
Guixin Yan ◽  
Shizhi Huang ◽  
Yongxin Geng ◽  
Mario Soberón ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Genome Sequencing ◽  
Insecticidal Activity ◽  
Insect Pest ◽  
Anoplophora Glabripennis ◽  
Insecticidal Proteins ◽  
Holotrichia Parallela ◽  
Larval Stages ◽  
Differential Toxicity

Scarabaeoidea and Chrysomeloidea insects are agriculture-destructive coleopteran pests. Few effective Bacillus thuringiensis (Bt) insecticidal proteins against these species have been described. Bt isolate BtSU4 was found to be active against coleopteran insects. Genome sequencing revealed two new cry8 genes in BtSU4, designated as cry8Ha1 and cry8Ia1. Both genes expressed a 135 kDa protoxin forming irregular shape crystals. Bioassays performed with Cry8Ha1 protoxin showed that it was toxic to both larvae and adult stages of Holotrichia parallela, also to Holotrichia oblita adults and to Anoplophora glabripennis larvae, but was not toxic to larval stages of H. oblita or Colaphellus bowringi. The Cry8Ia1 protoxin only showed toxicity against H. parallela larvae. After activation with chymotrypsin, the Cry8Ha1 activated toxin lost its insecticidal activity against H. oblita adults and reduced its activity on H. parallela adults, but gained toxicity against C. bowringi larvae, a Chrysomeloidea insect pest that feeds on crucifer crops. The chymotrypsin activated Cry8Ia1 toxin did not show toxicity to any one of these insects. These data show that Cry8Ha1 and Cry8Ia1 protoxin and activated toxin proteins have differential toxicity to diverse coleopteran species, and that protoxin is a more robust protein for the control of coleopteran insects.

Sign in / Sign up

Export Citation Format

Share Document