Morphological and molecular identification of  Phlebia wuliangshanensis sp. nov. in China

A new white-rot fungus, Phlebia wuliangshanensis, is proposed based on a combination of morphological features and molecular evidence. The species is characterized by an annual growth habit, resupinate basidiocarps with a smooth to tuberculate hymenial surface, a monomitic hyphal system with thin- to thick-walled generative hyphae bearing simple septa, presence of cystidia, and narrow ellipsoid to ellipsoid basidiospores (5–6 × 3–3.7 μm). Our phylogenetic analyses of ITS and LSU nrRNA sequences performed with maximum likelihood, maximum parsimony, and Bayesian inference methods support P. wuliangshanensis within a phlebioid clade in Meruliaceae (Polyporales). ITS+nLSU sequence analyses of additional Phlebia taxa strongly support P. wuliangshanensis within a monophyletic lineage grouped with P. chrysocreas and P. uda .

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Phlebia nigrodontea sp. nov. in Meruliaceae (Polyporales) with a black hymenial surface

Phytotaxa ◽

10.11646/phytotaxa.458.3.2 ◽

2020 ◽

Vol 458 (3) ◽

pp. 195-206

Author(s):

RUO-XIA HUANG ◽

KAI-YUE LUO ◽

CHANG-LIN ZHAO

Keyword(s):

Bayesian Inference ◽

Maximum Likelihood ◽

Phylogenetic Analyses ◽

Strong Support ◽

Molecular Data ◽

Molecular Evidence ◽

Monophyletic Lineage ◽

Phlebioid Clade ◽

Thin Walled ◽

Inference Methods

A new wood-inhabiting fungus, Phlebia nigrodontea, is proposed based on a combination of morphological features and molecular evidence. The species is characterized by a grandinioid hymenophore with vinaceous brown to black colour, a monomitic hyphal system with clamped generative hyphae and ellipsoid, colourless, thin-walled, smooth basidiospores (3.9–4.9 × 2.3–3.1 µm). Sequences of ITS and LSU nrRNA gene regions of the studied samples were generated, and phylogenetic analyses carried out using maximum likelihood, maximum parsimony and Bayesian inference methods. The phylogenetic analyses based on the molecular data of ITS+nLSU sequences showed that P. nigrodontea nested within the phlebioid clade. A further investigation of more representative taxa from Phlebia, based on ITS+nLSU sequences, demonstrated that the species P. nigrodontea formed a monophyletic lineage with strong support (100% BS, 100% BT, 1.00 BPP) and closely grouped with P. chrysocreas.

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Phlebia ailaoshanensis sp. nov. (Polyporales, Basidiomycota) evidenced by morphological characters and phylogenetic analyses

Phytotaxa ◽

10.11646/phytotaxa.373.3.2 ◽

2018 ◽

Vol 373 (3) ◽

pp. 184 ◽

Cited By ~ 2

Author(s):

SHAN SHEN ◽

XIANG MA ◽

TAI-MIN XU ◽

CHANG-LIN ZHAO

Keyword(s):

Growth Habit ◽

Phylogenetic Analyses ◽

Strong Support ◽

Morphological Characters ◽

Molecular Data ◽

Molecular Evidence ◽

Monophyletic Lineage ◽

Phlebioid Clade ◽

Thin Walled ◽

Inference Methods

A new wood-inhabiting fungus, Phlebia ailaoshanensis, is proposed based on a combination of morphological features and molecular evidence. The species is characterized by an annual growth habit, resupinate basidiocarps with tuberculate to phlebioid hymenial surface, a monomitic hyphal system with slightly thick-walled generative hyphae bearing simple septa, IKI–, CB– and ellipsoid, hyaline, thin-walled, smooth, IKI–, CB– basidiospores measuring as 5.7–8.5 × 3–4.3 µm. Sequences of ITS and LSU nrRNA gene regions of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and bayesian inference methods. The phylogenetic analyses based on molecular data of ITS+nLSU sequences showed that P. ailaoshanensis belonged to the Meruliaceae and nested into the phlebioid clade. Further investigation was obtained for more representative taxa in the Phlebia based on ITS+nLSU sequences, in which the result demonstrated that the species P. ailaoshanensis formed a monophyletic lineage with a strong support (100% BS, 100% BP, 1.00 BPP) and then grouped with P. acanthocystis, P. chrysocreas, P. ludoviciana, P. subcretacea and P. uda.

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Heteroradulum yunnanensis sp. nov. (Auriculariales, Basidiomycota) evidenced by morphological characters and phylogenetic analyses in China

Phytotaxa ◽

10.11646/phytotaxa.437.2.1 ◽

2020 ◽

Vol 437 (2) ◽

pp. 51-59

Author(s):

QIAN-XIN GUAN ◽

CHAO-MAO LIU ◽

TANG-JIE ZHAO ◽

CHANG-LIN ZHAO

Keyword(s):

Growth Habit ◽

Phylogenetic Analyses ◽

Strong Support ◽

Morphological Characters ◽

Molecular Data ◽

Fungal Species ◽

Molecular Evidence ◽

Monophyletic Lineage ◽

Thin Walled ◽

Inference Methods

A new wood-inhabiting fungal species, Heteroradulum yunnanensis, is proposed based on a combination of morphological features and molecular evidence. The species is characterized by an annual growth habit, resupinate basidiomata with odontoid hymenial surface (50–100 µm long), more or less pronounced yellow stains in older basidiomata, a monomitic hyphal system with thin-walled, clamped generative hyphae and two to three-celled basidia and cylindrical, hyaline, thin-walled, smooth, IKI–, CB– basidiospores measuring as 17–24 ×5–8 µm. Sequences of ITS and LSU nrRNA gene regions of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and bayesian inference methods. The phylogenetic analyses based on molecular data of ITS+nLSU sequences showed that Heteroradulum yunnanensis formed a monophyletic lineage with a strong support (100% BS, 100% BP, 1.00 BPP) and then grouped with H. adnatum.

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The Hidden Wood-Decaying Fungal Diversity: Rhizochaete from East Asia

Diversity ◽

10.3390/d13100503 ◽

2021 ◽

Vol 13 (10) ◽

pp. 503

Author(s):

Zi-Rui Gu ◽

Chang-Lin Zhao

Keyword(s):

New Species ◽

Bayesian Inference ◽

Fungal Diversity ◽

Forest Ecosystems ◽

Phylogenetic Analyses ◽

Molecular Evidence ◽

Corticioid Fungi ◽

Monophyletic Lineage ◽

Two New Species ◽

Inference Methods

Wood-decaying fungi play crucial roles as decomposers in forest ecosystems. In this study, two new corticioid fungi, Rhizochaete fissurata and R. grandinosa spp. nov., are proposed based on a combination of morphological features and molecular evidence. Rhizochaete fissurata is characterized by resupinate basidiomata with a cracking hymenial surface, a monomitic hyphal system with simple-septa generative hyphae, presence of subfusiform to conical cystidia encrusted at the apex or coarse on the upper half, and ellipsoid basidiospores. Rhizochaete grandinosa differs in its resupinate basidiomata with a smooth hymenial surface, presence of two types of cystidia, and ellipsoid basidiospores. Sequences of ITS and nLSU rRNA markers of the studied samples were employed, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony, and Bayesian inference methods on two datasets (ITS+nLSU and ITS). Both dataset analyses showed that two new species clustered into the genus Rhizochaete, in which, based on the ITS+nLSU dataset, R. fissurata was sister to R. belizensis, and R. grandinosa grouped with R. radicata; the phylogram inferred from ITS sequences inside Rhizochaete indicated that R. fissurata formed a monophyletic lineage with a lower support; R. grandinosa grouped closely with R. radicata. In addition, an identification key to all Rhizochaete species worldwide is provided.

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Septobasidium aquilariae sp. nov. from Yunnan, China

Mycotaxon ◽

10.5248/134.517 ◽

2019 ◽

Vol 134 (3) ◽

pp. 517-528

Author(s):

Rui-Ling Ma ◽

Meng Gao ◽

Xiang-Lin Zhuang ◽

Chang-Lin Zhao

Keyword(s):

Bayesian Inference ◽

Internal Transcribed Spacer ◽

Growth Habit ◽

Fungal Species ◽

Molecular Evidence ◽

Aquilaria Sinensis ◽

Internal Transcribed Spacer Region ◽

Monophyletic Lineage ◽

Thin Walled ◽

Inference Methods

A new fungal species, Septobasidium aquilariae, is proposed based on a combination of morphological features and molecular evidence. The species is characterized by an annual growth habit; a resupinate coriaceous basidiocarp with a cream to pale brown surface; a monomitic hyphal system with thick-walled generative hyphae bearing simple septa; reniform, hyaline, thin-walled, smooth basidiospores measuring 11–19 × 4–7.5 μm; and haustoria consisting of irregularly coiled hyphae. The fungus was found associated with Pseudaulacaspis sp. on Aquilaria sinensis. Sequences of internal transcribed spacer region (ITS) were analysed maximum likelihood, maximum parsimony, and Bayesian inference methods. The phylogenies strongly supported S. aquilariae in a monophyletic lineage (ML = 100%; MP = 100%; PP = 1) and grouped with "S. cokeri".

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Xylodon bambusinus and X. xinpingensis spp. nov. (Hymenochaetales) from southern China

Phytotaxa ◽

10.11646/phytotaxa.511.3.3 ◽

2021 ◽

Vol 511 (3) ◽

Author(s):

XIANG MA ◽

CHANG-LIN ZHAO

Keyword(s):

New Species ◽

Bayesian Inference ◽

Maximum Likelihood ◽

Growth Habit ◽

Southern China ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Two New Species ◽

Thin Walled ◽

Inference Methods

Two new species, Xylodon bambusinus and X. xinpingensis, are proposed based on morphological and molecular evidences. Both species share the annual growth habit, resupinate basidiomata and monomitic hyphal system with clamped, colorless generative hyphae, smooth, thin-walled basidiospores, but X. bambusinus is characterized by the smooth to tuberculate hymenial surface, presence of capitate and fusiform cystidia, broad ellipsoid basidiospores, while X. xinpingensis by the reticulate hymenophore with cream hymenial surface, and subglobose basidiospores (4.5–6 × 3.5–5 µm). Sequences of ITS and LSU nrRNA gene regions of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and Bayesian inference methods. The phylogenetic analyses based on molecular data of ITS and ITS+nLSU sequences showed that X. bambusinus was sister to X. subclavatus, while X. xinpingensis grouped with X. astrocystidiatus and X. paradoxus. The nLSU dataset revealed that X. bambusinus grouped with X. asperus and X. brevisetus with lower supports, and that X. xinpingensis grouped with X. astrocystidiatus and X. paradoxus and then with X. rimosissimus without supports. Both morphological and molecular evidences confirmed the placement of two new species in Xylodon. Description and figures from the new species and a key to the known species of Xylodon from China are presented.

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Gloeodontia yunnanensis sp. nov. (Russulales, Basidiomycota) from China, evidenced by morphological characters and phylogenetic analyses

Phytotaxa ◽

10.11646/phytotaxa.432.2.1 ◽

2020 ◽

Vol 432 (2) ◽

pp. 111-118

Author(s):

LU CHEN ◽

ZHENG-JUN SHI ◽

CHUN-HUA WU ◽

CHANG-LIN ZHAO

Keyword(s):

Bayesian Inference ◽

Maximum Likelihood ◽

Maximum Parsimony ◽

Phylogenetic Analyses ◽

Strong Support ◽

Morphological Characters ◽

Fungal Species ◽

Monophyletic Lineage ◽

Thin Walled ◽

Inference Methods

A new wood-inhabiting fungal species, Gloeodontia yunnanensis, is proposed based on a combination of morphological features and DNA data. The species is characterized by an annual, resupinate basidiomata with smooth hymenial surface, a monomitic hyphal system with thin-walled, clamped generative hyphae and obclavate cystidia and subglobose to globose, hyaline, thick-walled, asperulate, strongly amyloid, acyanophilous basidiospores measuring 3.3–4.3 × 2.5–3.5 µm. Sequences of ITS and 28S gene regions of the studied samples were generated and phylogenetic analyses were performed with Maximum Likelihood, Maximum Parsimony and Bayesian Inference methods. The analyses based on ITS+28S sequences showed that G. yunnanensis nested in the Gloeodontia clade and formed a monophyletic lineage with strong support (100% BS, 100% BP, 1.00 BPP).

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The Phylogenetic Relationship Revealed Three New Wood-Inhabiting Fungal Species From Genus Trechispora

Frontiers in Microbiology ◽

10.3389/fmicb.2021.650195 ◽

2021 ◽

Vol 12 ◽

Author(s):

Wei Zhao ◽

Chang-Lin Zhao

Keyword(s):

Bayesian Inference ◽

Maximum Likelihood ◽

Maximum Parsimony ◽

Internal Transcribed Spacer ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Fungal Species ◽

Molecular Evidence ◽

Wood Degradation ◽

Inference Methods

Wood-inhabiting fungi play a significant role in wood degradation and the cycle of matter in the ecological system. In the present study, three new wood-inhabiting fungal species, Trechispora bambusicola, Trechispora fimbriata, and Trechispora fissurata spp. nov., are nested in Trechispora, which are proposed based on a combination of morphological features and molecular evidence. Sequences of internal transcribed spacer (ITS) and large subunit (nLSU) regions of the studied samples were generated, and the phylogenetic analyses were performed with maximum likelihood, maximum parsimony, and Bayesian inference methods. The phylogenetic analyses inferred from ITS showed that T. bambusicola was sister to Trechispora stevensonii, T. fimbriata grouped with Trechispora nivea, and T. fissurata grouped with Trechispora echinospora. The phylogenetic tree based on ITS + nLSU sequences demonstrated that T. bambusicola formed a single lineage and then grouped with Trechispora rigida and T. stevensonii. T. fimbriata was sister to T. nivea. T. fissurata grouped with Trechispora thelephora.

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Rhomboidia wuliangshanensis gen. & sp. nov. from southwestern China

Mycotaxon ◽

10.5248/134.649 ◽

2020 ◽

Vol 134 (4) ◽

pp. 649-662

Author(s):

Tai-Min Xu ◽

Xiang-Fu Liu ◽

Yu-Hui Chen ◽

Chang-Lin Zhao

Keyword(s):

Yunnan Province ◽

Southwest China ◽

Phylogenetic Analyses ◽

White Rot ◽

Southwestern China ◽

Molecular Evidence ◽

Hyphal System ◽

Monophyletic Lineage ◽

Nuclear Rna ◽

Fungal Genus

A new, white-rot, poroid, wood-inhabiting fungal genus, Rhomboidia, typified by R. wuliangshanensis, is proposed based on morphological and molecular evidence. Collected from subtropical Yunnan Province in southwest China, Rhomboidia is characterized by annual, stipitate basidiomes with rhomboid pileus, a monomitic hyphal system with thick-walled generative hyphae bearing clamp connections, and broadly ellipsoid basidiospores with thin, hyaline, smooth walls. Phylogenetic analyses of ITS and LSU nuclear RNA gene regions showed that Rhomboidia is in Steccherinaceae and formed as distinct, monophyletic lineage within a subclade that includes Nigroporus, Trullella, and Flabellophora .

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Tuberculina photiniae sp. nov. (Helicobasidiales, Basidiomycota) supported by morphological characteristics and phylogenetic data

Phytotaxa ◽

10.11646/phytotaxa.317.2.3 ◽

2017 ◽

Vol 317 (2) ◽

pp. 113 ◽

Cited By ~ 1

Author(s):

YU-MEI ZHAO ◽

JIN XIE ◽

MENG-JIE LI ◽

LING ZHU ◽

TONG-XIN ZHOU ◽

...

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Phylogenetic Analyses ◽

Morphological Characteristics ◽

Molecular Data ◽

Fungal Species ◽

Molecular Evidence ◽

Monophyletic Lineage ◽

Leaf Surfaces ◽

Inference Methods

A new mycoparasitic fungal species, Tuberculina photiniae sp. nov., is proposed based on morphological and molecular evidence. The species is characterized by its depressed hemispherical pale lilac to greyish purple sporodochia breaking through leaf surfaces, septated conidiophores with vinaceous lower parts and colourless upper parts, and the presence of colourless globose, subglobose or scarcely wide-ellipsoid conidia. Sequences of ITS and LSU nrRNA gene regions of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and Bayesian inference methods. The phylogenetic analysis based on molecular data of ITS sequences showed that Tuberculina photiniae belongs to the Tuberculina-Helicobasidium clade and is closely related to H. longisporum and H. mompa. Further investigation was carried out for representative taxa in the Tuberculina-Helicobasidium group based on ITS+nLSU sequences in which the results demonstrated that the new species formed a monophyletic lineage and grouped with H. longisporum and H. mompa. Both morphological and molecular evidence confirmed the placement of the new species in the Tuberculina-Helicobasidium clade.

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