Phlebia nigrodontea sp. nov. in Meruliaceae (Polyporales) with a black hymenial surface

A new wood-inhabiting fungus, Phlebia nigrodontea, is proposed based on a combination of morphological features and molecular evidence. The species is characterized by a grandinioid hymenophore with vinaceous brown to black colour, a monomitic hyphal system with clamped generative hyphae and ellipsoid, colourless, thin-walled, smooth basidiospores (3.9–4.9 × 2.3–3.1 µm). Sequences of ITS and LSU nrRNA gene regions of the studied samples were generated, and phylogenetic analyses carried out using maximum likelihood, maximum parsimony and Bayesian inference methods. The phylogenetic analyses based on the molecular data of ITS+nLSU sequences showed that P. nigrodontea nested within the phlebioid clade. A further investigation of more representative taxa from Phlebia, based on ITS+nLSU sequences, demonstrated that the species P. nigrodontea formed a monophyletic lineage with strong support (100% BS, 100% BT, 1.00 BPP) and closely grouped with P. chrysocreas.

Phlebiopsis lacerata sp. nov. (Polyporales, Basidiomycota) from southern China

A new white-rot species, Phlebiopsis lacerata sp. nov., is proposed based on morphological and molecular characters. It is characterized by resupinate to effuse-reflexed basidiomata, a monomitic hyphal system with simple septate generative hyphae, conical cystidia that are thick-walled, heavily encrusted with large crystal in the apical part, and ellipsoid basidiospores (4–5.5 × 3–3.5 µm). Phylogenetic analyses of the ITS and LSU nrRNA gene regions showed that P. lacerata belongs to the Phanerochaetaceae and is nested in the phlebioid clade. Further investigation based on ITS+nLSU sequences with more representative taxa in Phlebiopsis demonstrated that P. lacerata forms a monophyletic lineage with a strong support (100% BS, 100% BP, 1.00 BPP) and then groups with P. crassa.

Morphological and molecular identification of Phlebia wuliangshanensis sp. nov. in China

A new white-rot fungus, Phlebia wuliangshanensis, is proposed based on a combination of morphological features and molecular evidence. The species is characterized by an annual growth habit, resupinate basidiocarps with a smooth to tuberculate hymenial surface, a monomitic hyphal system with thin- to thick-walled generative hyphae bearing simple septa, presence of cystidia, and narrow ellipsoid to ellipsoid basidiospores (5–6 × 3–3.7 μm). Our phylogenetic analyses of ITS and LSU nrRNA sequences performed with maximum likelihood, maximum parsimony, and Bayesian inference methods support P. wuliangshanensis within a phlebioid clade in Meruliaceae (Polyporales). ITS+nLSU sequence analyses of additional Phlebia taxa strongly support P. wuliangshanensis within a monophyletic lineage grouped with P. chrysocreas and P. uda .

Phlebia ailaoshanensis sp. nov. (Polyporales, Basidiomycota) evidenced by morphological characters and phylogenetic analyses

A new wood-inhabiting fungus, Phlebia ailaoshanensis, is proposed based on a combination of morphological features and molecular evidence. The species is characterized by an annual growth habit, resupinate basidiocarps with tuberculate to phlebioid hymenial surface, a monomitic hyphal system with slightly thick-walled generative hyphae bearing simple septa, IKI–, CB– and ellipsoid, hyaline, thin-walled, smooth, IKI–, CB– basidiospores measuring as 5.7–8.5 × 3–4.3 µm. Sequences of ITS and LSU nrRNA gene regions of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and bayesian inference methods. The phylogenetic analyses based on molecular data of ITS+nLSU sequences showed that P. ailaoshanensis belonged to the Meruliaceae and nested into the phlebioid clade. Further investigation was obtained for more representative taxa in the Phlebia based on ITS+nLSU sequences, in which the result demonstrated that the species P. ailaoshanensis formed a monophyletic lineage with a strong support (100% BS, 100% BP, 1.00 BPP) and then grouped with P. acanthocystis, P. chrysocreas, P. ludoviciana, P. subcretacea and P. uda.

Three new species of Phanerochaete (Polyporales, Basidiomycota)

Phanerochaetecanobrunnea, P.cystidiata and P.fusca are presented as new species, supported by morphological studies and two sets of phylogenetic analyses. The 5.8S+nuc 28S+rpb1 dataset shows the generic placement of the three species within the phlebioid clade of Polyporales. The ITS+nuc 28S dataset displays relationships for the new taxa within Phanerochaete s.s. Phanerochaetecanobrunnea grew on angiosperm branches in subtropical Taiwan and is characterised by greyish brown hymenial surface, brown generative hyphae and skeletal hyphae and absence of cystidia. Phanerochaetecystidiata grew on angiosperm branches above 1000 m in montane Taiwan and SW Yunnan Province of China and is characterised by cream to yellowish hymenial surface and more or less encrusted leptocystidia. Phanerochaetefusca grew on angiosperm branches at 1700 m in Hubei Province of China and is characterised by dark brown hymenial surface, leptocystidia, brown subicular hyphae and colourless to brownish basidiospores.

Reappraisal of Climacodon (Basidiomycota, Meruliaceae) and reinstatement of Donkia (Phanerochaetaceae) using multigene data

Climacodon comprises species characterized by a hydnoid hymenophore, a monomitic hyphal system and spores without iodine reactions. Climacodon pulcherrimus is unique within Climacodon due to having multiple clamp connections. Current taxonomy of Climacodon relies exclusively on treatments based on morphological observations. In this study, we constructed a 6-locus DNA phylogeny (LSU, 5.8S, SSU, RPB2, ef-1α, RPB1) to evaluate the monophily of Climacodon. Our results show that Climacodon is polyphyletic as currently circumscribed. Climacodon sanguineus forms a well-supported clade with C. septentrionale, type species of Climacodon, and nests in the Phlebioid clade. A third species, Climacodon roseomaculatus, is closely related to C. sanguineus based on ITS sequence data. Hence, these three species belong to Climacodon s. str., which is characterized by having thick-walled crystalliferous cystidia. Meruliaceae is adopted as family placement for Climacodon s. str. and the whole Phlebioid clade. On the contrary, Climacodon pulcherrimus is nested in the Phanerochaete clade and better accommodated in the monotypic genus Donkia, within Phanerochaetaceae. The presence of multiple clamps by septum in C. pulcherrimus is shared with some Phanerochaete species of this clade. Recent collections of C. sanguineus were compared with the type material, and coloured photographs of macro- and microscopic features of C. sanguineus and Donkia pulcherrima are provided.