Gymnopus barbipes and G. dysodes, new records for Pakistan

Mycotaxon ◽  
2020 ◽  
Vol 135 (1) ◽  
pp. 203-212
Author(s):  
Malka Saba ◽  
Junaid Khan ◽  
Samina Sarwar ◽  
Hassan Sher ◽  
Abdul Nasir Khalid
Keyword(s):  
Phylogenetic Analysis ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
New Records ◽  
Phylogenetic Analyses ◽  
Internal Transcribed Spacer Region

Morphological and phylogenetic analyses were conducted to identify Gymnopus species collected in Pakistan during 2013–14. Phylogenetic analysis was performed using internal transcribed spacer region (ITS) of ribosomal DNA sequences. Among the collected taxa, we identified Gymnopus barbipes and G. dysodes, represent new records for Pakistan. Their detailed descriptions and illustrations are also provided.

scholarly journals A new concept for Dictyostelium sphaerocephalum based on morphology and phylogenetic analysis of nuclear ribosomal internal transcribed spacer region sequences

2007 ◽  
Vol 85 (1) ◽  
pp. 104-110 ◽  
Author(s):  
Maria Romeralo ◽  
Omar Fiz-Palacios ◽  
Carlos Lado ◽  
James C. Cavender
Keyword(s):  
Phylogenetic Analysis ◽  
Phylogenetic Relationships ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Morphological Plasticity ◽  
Branching Pattern ◽  
Classical Concept ◽  
Internal Transcribed Spacer Region

Three dictyostelid isolates were found in Spain and Argentina that are morphologically different from known species. These isolates have some features similar to Dictyostelium sphaerocephalum (Oudem.) Sacc., Marchal & É.J. Marchal, but differ in size and sorocarp branching pattern. We sequenced the nuclear ribosomal internal transcribed spacer region to explore phylogenetic relationships among this group of species, including the three new isolates and their closest relatives. In all phylogenetic analyses performed, sequences of all three isolates group together with sequences from “typical” D. sphaerocephalum samples. This result supports previous observations of the morphological plasticity in dictyostelids, especially D. sphaerocephalum, leading us to broaden the classical concept of this species.

Phylogenetic analyses of Nematoctonus and Hohenbuehelia (Pleurotaceae)

2007 ◽  
Vol 85 (8) ◽  
pp. 762-773 ◽  
Author(s):  
Alexandra T.E. Koziak ◽  
Kei Chin Cheng ◽  
R. Greg Thorn
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Nuclear Ribosomal Dna ◽  
Monophyletic Clade ◽  
Parasitoid Species ◽  
Internal Transcribed Spacer Region ◽  
Species Limits ◽  
Additional Gene

Hohenbuehelia (Agaricales, Pleurotaceae) and Nematoctonus (Hyphomycetes) are the names for the sexual and asexual stages of a genus of nematode-destroying fungi (Basidiomycota). We obtained partial sequences of nuclear ribosomal DNA, including the internal transcribed spacer region and the 5′ end of the large subunit, of 37 isolates of Hohenbuehelia and Nematoctonus representing 13 of the 16 described species in Nematoctonus. Phylogenetic analyses support Hohenbuehelia–Nematoctonus as a monophyletic clade of the Pleurotaceae, within which the species were placed in five main subclades. Exclusively predatory species ( Nematoctonus brevisporus Thorn & G.L. Barron, Nematoctonus campylosporus Drechsler, Nematoctonus robustus F.R. Jones, and Nematoctonus sp. UAMH 5317) appear to be basal. In these species, adhesive knobs to capture prey are produced on their hyphae but not on their conidia. A single mycelial individual may feed on many nematodes. From these have arisen both exclusively parasitoid species ( Nematoctonus cylindrosporus Thorn & G.L. Barron, Nematoctonus leiosporus Drechsler, Nematoctonus leptosporus Drechsler, Nematoctonus pachysporus Drechsler, Nematoctonus tylosporus Drechsler), and species that we call intermediate predators ( Nematoctonus angustatus Thorn & G.L. Barron, Nematoctonus concurrens Drechsler, Nematoctonus geogenius Thorn & GL. Barron, Nematoctonus hamatus Thorn & G.L. Barron, and Nematoctonus subreniformis Thorn & G.L. Barron). Exclusively parasitoid species have conidia that germinate to form sticky knobs that attach to passing nematodes but lack adhesive knobs on the hyphae. Each mycelial individual feeds on only one nematode. Intermediate predators have adhesive knobs both on hyphae and on germinated conidia and can act in both predatory and parasitoid modes. Most morphospecies are resolved as monophyletic, but sequences of additional gene regions are required to clarify species limits within the N. angustatus – N. geogenius group.

Phylogenetic analysis of Phylica L. ( Rhamnaceae ) with an emphasis on island species: evidence from plastid trnL‐F and nuclear internal transcribed spacer (ribosomal) DNA sequences

Taxon ◽  
2001 ◽  
Vol 50 (2) ◽  
pp. 405-427 ◽  
Author(s):  
James E. Richardson ◽  
Frans M. Weitz ◽  
Michael F. Fay ◽  
Quentin C. B. Cronk ◽  
H. Peter Linder ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
Island Species

Kamalia indica, a new genus and species belonging to Diaporthales (Ascomycota, Fungi) described based on morphology and molecular phylogeny

Phytotaxa ◽  
2019 ◽  
Vol 425 (4) ◽  
pp. 233-243
Author(s):  
SHIWALI RANA ◽  
SANJAY KUMAR SINGH
Keyword(s):  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
New Genus ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Type Specimen ◽  
Morphological Description ◽  
Fungal Culture ◽  
Internal Transcribed Spacer Region

A fungal taxa isolated from leaf spots of Mallotus philippensis from Kangra district of North-Western Himalayan region of India is established as a new genus based on morphological characters of asexual-morphs, cultural characteristics and phylogenetic analyses of the partial nuclear ribosomal 28S large subunit (LSU) and internal transcribed spacer (ITS) rDNA sequence data. The generic placement of the genus has been determined based on DNA sequences from authenticated isolates. The present taxon has turned out to be distinct, showing nearly 90% identity with other known genera in Diaporthales based on nrDNA internal transcribed spacer region. The morphological description is provided for the new taxa and compared with the similar taxa belonging to the order Diaporthales. The culture was found to show heavy sporulation in all kind of media. The type specimen and ex type culture have been deposited in the Ajrekar Mycological Herbarium (AMH) and National Fungal Culture Collection of India (NFCCI-WDCM 932), respectively.

scholarly journals Molecular phylogenetic analyses of Drakaeinae: Diurideae (Orchidaceae) based on DNA sequences of the internal transcribed spacer region

2014 ◽  
Vol 27 (1) ◽  
pp. 3 ◽  
Author(s):  
Joseph T. Miller ◽  
Mark A. Clements
Keyword(s):  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Internal Transcribed Spacer Region ◽  
Orchid Species ◽  
Weak Support ◽  
Separate Genus ◽  
Rdna Sequences ◽  
Molecular Phylogenetic ◽  
Taxonomic Summary

Results of the analysis of rDNA sequences based on 55 collections representative of 32 Drakaeinae orchid species and outgroups supported the monophyly of the subtribe, with weak support for the inclusion of Spiculaea, and revealed six strongly supported monophyletic, well defined morphological groups. Caleana is monophyletic. Chiloglottis s.lat. is monophyletic when Simpliglottis and Myrmechila are included. Our results also suggested that the segregate genus Phoringopsis is better treated as part of Arthrochilus.There is sufficient molecular and morphological support for recognition of the leafless, mycroheterotrophic Thynninorchis to be maintained as a separate genus. A taxonomic summary is provided, including reassignment of taxa at generic ranks and new combinations for Caleana alcockii (Hopper & A.P.Br.) M.A.Clem., Caleana brockmanii (Hopper & A.P.Br.) M.A.Clem., Caleana disjuncta (D.L.Jones) M.A.Clem., Caleana dixonii (Hopper & A.P.Br.) M.A.Clem., Caleana gracilicordata (Hopper & A.P.Br.) M.A.Clem., Caleana granitica (Hopper & A.P.Br.) M.A.Clem., Caleana hortiorum (Hopper & A.P.Br.) M.A.Clem., Caleana lyonsii (Hopper & A.P.Br.) M.A.Clem., Caleana parvula (Hopper & A.P.Br.) M.A.Clem., Caleana terminalis (Hopper & A.P.Br.) M.A.Clem. and Caleana triens (Hopper & A.P.Br.) M.A.Clem.

Phaeoclavulina pseudozippelii sp. nov. (Gomphales, Basidiomycota) from Northern Thailand

Phytotaxa ◽  
2018 ◽  
Vol 362 (2) ◽  
pp. 211
Author(s):  
NOPPARAT WANNATHES ◽  
RATTIKAN KAEWKETSRI ◽  
NAKARIN SUWANNARACH ◽  
JATURONG KUMLA ◽  
SAISAMORN LUMYONG
Keyword(s):  
Ribosomal Dna ◽  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
National Park ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Taxonomic Position ◽  
Northern Thailand ◽  
Coral Fungi

Coral fungi in Phu Hin Rong Kla National Park, Thailand, were surveyed from June to September of 2011 and 2012. A novel species, Phaeoclavulina pseudozippelii is described together with illustrations, photographs, and a comparison with phenetically similar taxa. Its taxonomic position was confirmed through phylogenetic analyses of the large subunit and internal transcribed spacer (ITS1-5.8S-ITS2) ribosomal DNA sequences.

scholarly journals Krefftascaris (Nematoda, Ascaridoidea) from Australian Side-Necked Turtles with Description of Krefftascaris Sharpiloi SP. N. from Chelodina Rugosa

2010 ◽  
Vol 44 (1) ◽  
pp. e-1-e-11 ◽  
Author(s):  
V. Tkach ◽  
Yu. Kuzmin ◽  
S. Snyder
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
New Records ◽  
Ribosomal Subunit ◽  
Northern Territory ◽  
Nerve Ring ◽  
Subunit Gene ◽  
Molecular Phylogenetic

Krefftascaris(Nematoda, Ascaridoidea) from Australian Side-Necked Turtles with Description ofKrefftascaris SharpiloiSP. N. fromChelodina RugosaPreviously known records of ascaridoid nematodesKrefftascarisSprent, 1980 are summarized and new records of the genus reported.Krefftascaris sharpiloiTkach, Kuzmin et Snyder, sp. n. is described from specimens found in the stomach of the northern snake-necked turtleChelodina rugosacollected from two localities in Northern Territory, Australia. The new species differs from the only previously knownKrefftascarisspecies,K. parmenteriSprent, 1980, by the presence of thickened and bifurcated anterior edges of the lateral cuticular alae and a difference in the relative distance from the anterior end to the nerve ring which is 1.5 to 2 times greater inK. parmenteri.Comparison of approximately 2.100 bases of ribosomal DNA sequences This study contains first reports ofKrefftascarisinChelodina rugosa, Chelodina burrungandjii, Chelodina canniandEmydura tanybaragaand the first records of this genus in the Northern Territory, Queensland and Western Australia. Molecular phylogenetic analysis based on sequences of nuclear small ribosomal subunit gene has demonstrated close affinities betweenKrefftascarisandHeterocheilus, the type genus of the Heterocheilidae and Heterocheilinae. Parasitism of several species and genera of Heterocheilidae in crocodiles allows us to hypothesize thatKrefftascarismay have been acquired by turtles from crocodilians.

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