Checklist of powdery mildew fungi (Erysiphaceae) in Pakistan

Mycotaxon ◽  
2021 ◽  
Vol 136 (3) ◽  
pp. 689-689
Author(s):  
N.S. Afshan ◽  
I. Zafar ◽  
A.N. Khalid
Keyword(s):  
Powdery Mildew ◽  
Species List ◽  
Powdery Mildew Fungi

The 10-page "Checklist of powdery mildew fungi (Erysiphaceae) in Pakistan " by Afshan, Zafar, and Khalid may now be downloaded from Mycotaxon's mycobiota webpage. This annotated species list brings to 149 the number of freeaccess fungae now available on our website: http://www.mycotaxon.com/mycobiota/index.html

New records of microcyclic conidiogenesis in some powdery mildew fungi

Mycoscience ◽  
2011 ◽  
Vol 52 (3) ◽  
pp. 213-216 ◽  
Author(s):  
Alexandra Pintye ◽  
Sara Elisabetta Legler ◽  
Levente Kiss
Keyword(s):  
Powdery Mildew ◽  
New Records ◽  
Powdery Mildew Fungi

Development of a diagnostic assay for race differentiation of Podosphaera macularis

Plant Disease ◽  
2020 ◽  
Author(s):  
Mary Block ◽  
Brian Knaus ◽  
Michele S. Wiseman ◽  
Niklaus J. Grünwald ◽  
David H. Gent
Keyword(s):  
Powdery Mildew ◽  
Pacific Northwest ◽  
The United States ◽  
Epidemiological Studies ◽  
Locked Nucleic Acid ◽  
Nucleotide Polymorphisms ◽  
Practical Applications ◽  
Powdery Mildew Fungi ◽  
Perfect Discrimination ◽  
Podosphaera Macularis

Hop powdery mildew (caused by Podosphaera macularis) was confirmed in the Pacific Northwest in 1996. Before 2012, the most common race of P. macularis was able to infect plants that possessed powdery mildew resistance based on the R-genes Rb, R3, and R5. After 2012, two additional races of P. macularis were discovered that can overcome the resistance gene R6 and the partial resistance found in the cultivar Cascade. These three races now occur throughout the region, which can complicate management and research efforts because of uncertainty on which race(s) may be present in the region and able to infect susceptible hop genotypes. Current methods for determining the races of P. macularis are labor intensive, costly, and typically require more than 14 days to obtain results. We sought to develop a molecular assay to differentiate races of the fungus possessing virulence on plants with R6, referred to as V6-virulent, from other races. The transcriptomes of 46 isolates of P. macularis were sequenced to identify loci and variants unique to V6-isolates. Fourteen primer pairs were designed for 10 candidate loci that contained single nucleotide polymorphisms (SNP) and short insertion-deletion polymorphisms. Two differentially-labeled locked nucleic acid probes were designed for a contig that contained a conserved SNP associated with V6-virulence. The resulting duplexed real-time PCR assay was validated against 46 V6 and 54 non-V6 P. macularis isolates collected from the United States and Europe. The assay had perfect discrimination of V6-virulence among isolates of P. macularis originating from the western U.S. but failed to predict V6-virulence in three isolates collected from Europe. The specificity of the assay was tested with different species of powdery mildew fungi and other microorganisms associated with hop. Weak non-specific amplification occurred with powdery mildew fungi collected from Vitis vinifera, Fragaria sp., and Zinnia sp.; however, non-specification amplification is not a concern when differentiating pathogen race from colonies on hop. The assay has practical applications in hop breeding, epidemiological studies, and other settings where rapid confirmation of pathogen race is needed.

Sign in / Sign up

Export Citation Format

Share Document