After clot formation using bovine thrombin and bovine fibrinogen, one can detect no longer the total amount of thrombin. The amount missing in the supernatant is trapped in the clot and can be removed in active state after lysing the clot. The capacity for trapping thrombin by a constant amount of fibrin depends on the structure of the fibrinpolymer. This can be shown by producing different kinds of clots by variation of pH or monomer concentration during polymerisation or using Arvinmonomer insted of Thrombinmonomer. The trapped thrombin shows no clotting activity but some activity with chromogenic substrats remains. to neutralize trapped thrombin about 5 times more antithrombin III is necessary than for the same amount of free thrombin. The trapped thrombin diffuses out of the clot indicating the reversibility of the binding of thrombin to fribin.