DNA engineering utilizing thymidylate synthase A (thyA) selection system in Escherichia coli

2001 ◽  
Author(s):  
Nga-yi, Queenie Wong
Keyword(s):  
Escherichia Coli ◽  
Thymidylate Synthase ◽  
Selection System ◽  
Dna Engineering

scholarly journals Catalytic role of histidine 147 in Escherichia coli thymidylate synthase

1989 ◽  
Vol 264 (32) ◽  
pp. 19132-19137
Author(s):  
I K Dev ◽  
B B Yates ◽  
J Atashi ◽  
W S Dallas
Keyword(s):  
Escherichia Coli ◽  
Thymidylate Synthase ◽  
Catalytic Role

scholarly journals Development of a selection system for the detection of L-ribose isomerase expressing mutants of Escherichia coli

2007 ◽  
Vol 76 (5) ◽  
pp. 1051-1057 ◽  
Author(s):  
Cassandra De Muynck ◽  
Jef Van der Borght ◽  
Marjan De Mey ◽  
Sofie L. De Maeseneire ◽  
Inge N. A. Van Bogaert ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Selection System

scholarly journals Expression of Human Thymidylate Synthase in Escherichia coli

1989 ◽  
Vol 264 (16) ◽  
pp. 9145-9148 ◽  
Author(s):  
V J Davisson ◽  
W Sirawaraporn ◽  
D V Santi
Keyword(s):  
Escherichia Coli ◽  
Thymidylate Synthase

scholarly journals Role of an invariant lysine residue in folate binding on Escherichia coli thymidylate synthase: Calorimetric and crystallographic analysis of the K48Q mutant

2008 ◽  
Vol 40 (10) ◽  
pp. 2206-2217 ◽  
Author(s):  
Aldo A. Arvizu-Flores ◽  
Rocio Sugich-Miranda ◽  
Rodrigo Arreola ◽  
Karina D. Garcia-Orozco ◽  
Enrique F. Velazquez-Contreras ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Thymidylate Synthase ◽  
Crystallographic Analysis ◽  
Lysine Residue

Kasugamycin inhibition of nonsense suppression by thymine-requiring strains of Escherichia coli K12

1993 ◽  
Vol 39 (4) ◽  
pp. 448-450 ◽  
Author(s):  
E. Tiganos ◽  
M. B. Herrington
Keyword(s):  
Escherichia Coli ◽  
Thymidylate Synthase ◽  
Aminoglycoside Antibiotic ◽  
Nonsense Suppression ◽  
Resistance Mutations ◽  
Escherichia Coli K12 ◽  
Translational Accuracy ◽  
Frame Shift

Thymine-requiring strains of Escherichia coli suppress nonsense and frame-shift mutations. This appears to occur during translation, suggesting that the lack of activity of an enzyme thymidylate synthase, required for the synthesis of a DNA precursor, alters the fidelity of translation. The aminoglycoside antibiotic kasugamycin, which enhances translational accuracy in vitro, prevents thymine-requiring cells from suppressing. The inhibition of suppression by kasugamycin is not prevented by the introduction of two different kasugamycin-resistance mutations, although the dose required for inhibition increases. These observations support the conclusion that suppression occurs during translation.Key words: suppression, kasugamycin, translation, thymine-requiring.

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