scholarly journals The umpA gene of Escherichia coli encodes phosphatidylglycerol:prolipoprotein diacylglyceryl transferase (lgt) and regulates thymidylate synthase levels through translational coupling.

1995 ◽  
Vol 177 (7) ◽  
pp. 1879-1882 ◽  
Author(s):  
K Gan ◽  
K Sankaran ◽  
M G Williams ◽  
M Aldea ◽  
K E Rudd ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Thymidylate Synthase ◽  
Translational Coupling

scholarly journals Catalytic role of histidine 147 in Escherichia coli thymidylate synthase

1989 ◽  
Vol 264 (32) ◽  
pp. 19132-19137
Author(s):  
I K Dev ◽  
B B Yates ◽  
J Atashi ◽  
W S Dallas
Keyword(s):  
Escherichia Coli ◽  
Thymidylate Synthase ◽  
Catalytic Role

scholarly journals Expression of Human Thymidylate Synthase in Escherichia coli

1989 ◽  
Vol 264 (16) ◽  
pp. 9145-9148 ◽  
Author(s):  
V J Davisson ◽  
W Sirawaraporn ◽  
D V Santi
Keyword(s):  
Escherichia Coli ◽  
Thymidylate Synthase

scholarly journals Role of an invariant lysine residue in folate binding on Escherichia coli thymidylate synthase: Calorimetric and crystallographic analysis of the K48Q mutant

2008 ◽  
Vol 40 (10) ◽  
pp. 2206-2217 ◽  
Author(s):  
Aldo A. Arvizu-Flores ◽  
Rocio Sugich-Miranda ◽  
Rodrigo Arreola ◽  
Karina D. Garcia-Orozco ◽  
Enrique F. Velazquez-Contreras ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Thymidylate Synthase ◽  
Crystallographic Analysis ◽  
Lysine Residue

Kasugamycin inhibition of nonsense suppression by thymine-requiring strains of Escherichia coli K12

1993 ◽  
Vol 39 (4) ◽  
pp. 448-450 ◽  
Author(s):  
E. Tiganos ◽  
M. B. Herrington
Keyword(s):  
Escherichia Coli ◽  
Thymidylate Synthase ◽  
Aminoglycoside Antibiotic ◽  
Nonsense Suppression ◽  
Resistance Mutations ◽  
Escherichia Coli K12 ◽  
Translational Accuracy ◽  
Frame Shift

Thymine-requiring strains of Escherichia coli suppress nonsense and frame-shift mutations. This appears to occur during translation, suggesting that the lack of activity of an enzyme thymidylate synthase, required for the synthesis of a DNA precursor, alters the fidelity of translation. The aminoglycoside antibiotic kasugamycin, which enhances translational accuracy in vitro, prevents thymine-requiring cells from suppressing. The inhibition of suppression by kasugamycin is not prevented by the introduction of two different kasugamycin-resistance mutations, although the dose required for inhibition increases. These observations support the conclusion that suppression occurs during translation.Key words: suppression, kasugamycin, translation, thymine-requiring.

scholarly journals Regulation of Escherichia coli secA by Cellular Protein Secretion Proficiency Requires an Intact Gene X Signal Sequence and an Active Translocon

1998 ◽  
Vol 180 (19) ◽  
pp. 5240-5242 ◽  
Author(s):  
Donald Oliver ◽  
Jessica Norman ◽  
Shameema Sarker
Keyword(s):  
Escherichia Coli ◽  
Protein Secretion ◽  
Signal Sequence ◽  
Cellular Protein ◽  
X Protein ◽  
Translational Coupling ◽  
Intact Gene

ABSTRACT secA is translationally regulated by the protein secretion proficiency state of the Escherichia coli cell. This regulation was explored by making signal sequence mutations in the gene upstream of secA, gene X, which promotessecA translational coupling. Gene X signal sequence mutants were constitutive for secA expression, whileprlA alleles partially restored secAregulation. These results show that interaction of the pre-gene X protein with the translocon is required for proper secAregulation. Furthermore, gene X signal sequence mutations disruptedsecA regulation only in the cis configuration. We propose that nascent pre-gene X protein interacts with the translocon during its secretion to constitute the secretion sensor.

Sign in / Sign up

Export Citation Format

Share Document